Phenotypic and functional analysis of multi-transgenic porcine GGTA1-deficient fibroblasts.
(A) Flow cytometry analysis of human CD46, CD55 and CD59 expression and loss of α-Gal epitopes in piglet 779 PKFs. Grey histograms represent secondary antibody staining only. (B) 51-Cr labelled PKF from wild-type and multi-transgenic GGTA1-deficient piglet 779 were incubated with concentrations of human serum as indicated. Shown is % specific lysis (mean ± SD) calculated from triplicate samples of a representative experiment.