Figure 4 | Scientific Reports

Figure 4

From: Efficient production of multi-modified pigs for xenotransplantation by ‘combineering’, gene stacking and gene editing

Figure 4

Phenotypic and functional analysis of multi-transgenic porcine GGTA1-deficient fibroblasts.

(A) Flow cytometry analysis of human CD46, CD55 and CD59 expression and loss of α-Gal epitopes in piglet 779 PKFs. Grey histograms represent secondary antibody staining only. (B) 51-Cr labelled PKF from wild-type and multi-transgenic GGTA1-deficient piglet 779 were incubated with concentrations of human serum as indicated. Shown is % specific lysis (mean ± SD) calculated from triplicate samples of a representative experiment.

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