(A) RT-PCR analysis of multi-transgenic GGTA1-deficient piglet 779 organs and cultured endothelial cells. Organs from piglet 779, piglet 1706 PKF, human MSC line SCP1 and wild-type porcine MSCs are indicated. Please note that the GAPDH primers used were specific to porcine samples, so human SCP1 showed no amplification. (B) RT-PCR analysis of CD46 splicing variants. PKFs from multi-transgenic piglet 1706 and multi-transgenic GGTA1-deficient piglet 779, human MSC line SCP1 and wild-type porcine MSCs are as indicated (C) RT-PCR analysis of CD55 RNA splicing variants. Lanes are as in B. RT-PCR bands indicated correspond to membrane-bound CD55 isoforms: gDAF, vDAF4 and vDAF5 and the soluble isoforms sDAF, vDAF1, vDAF2 and vDAF3 in PKF of transgenic pigs 1706 and 779 as described in normal human tissues26.