Figure 1: Light-Exposure Activates AhR Signaling in mammalian retina. | Scientific Reports

Figure 1: Light-Exposure Activates AhR Signaling in mammalian retina.

From: A novel AhR ligand, 2AI, protects the retina from environmental stress

Figure 1

(A–D) AhR staining of light exposed retinas. Retinal sections from C57Bl6 (control (A) and 6 hours after light (B)) and Balb/C mice (control (C) and 6 hours after light (D)) stained with DAPI (nuclear stain, blue) and an anti-AhR antibody (green) showing nuclear translocation of AhR. A’, A”, B’, B”, C’, C”, D’ and D” show zoomed-in views of the boxed regions in (A–D) demonstrating co-localization of AhR and DAPI in RPE and photoreceptor cells. (E–G) CYP1A staining (in green) of control (E-E”) and light exposed C57Bl6 retinas at 6 hours (F-F”) and at 24 hours (G-G”). E”, F” and G” are zoomed-in views of boxed regions in (E–G) and arrows in G” mark RPE cells expressing CYP1A. DAPI (blue) stains nuclei (H) Relative mRNA-levels of AhR targets in murine neural retina 12 hrs after light exposure. (I) Relative expression of AhR and its targets in hESC-derived RPE cells 12 hours after in vitro blue light (400 nm) exposure for 30–60 mins. (Scale bar = 20 μm).

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