Lytic infection with herpes simplex virus type 1 (HSV-1) induces profound modification of the cell nucleus including formation of a viral replication compartment and chromatin marginalization into the nuclear periphery. We used three-dimensional soft X-ray tomography, combined with cryogenic fluorescence, confocal and electron microscopy, to analyse the transformation of peripheral chromatin during HSV-1 infection. Our data showed an increased presence of low-density gaps in the marginalized chromatin at late infection. Advanced data analysis indicated the formation of virus-nucleocapsid-sized (or wider) channels extending through the compacted chromatin of the host. Importantly, confocal and electron microscopy analysis showed that these gaps frequently contained viral nucleocapsids. These results demonstrated that HSV-1 infection induces the formation of channels penetrating the compacted layer of cellular chromatin and allowing for the passage of progeny viruses to the nuclear envelope, their site of nuclear egress.
Herpes viruses target the cell nucleus because of their need for the cellular DNA reproduction machinery. The nuclear entry of viral DNA is followed by the nuclear accumulation of viral proteins and replication of viral DNA, leading to the formation of viral replication compartments (VRCs). Electron microscopy and confocal microscopy studies have illustrated how profoundly these viruses transform the nuclear structure so as to optimise their multiplication1,2,3,4,5.
During cellular entry the nucleocapsid of herpes simplex virus type-1 (HSV-1) undergoes partial disassembly at the nuclear pore complex (NPC), followed by viral genome delivery via the pore6,7. When the infection proceeds, the VRCs emerge as distinct nuclear foci, which subsequently undergo fusion and expansion into a large, globular VRC8,9,10,11. The infected cell expresses and multiples only a limited number of input HSV-1 genomes (fewer than eight at a multiplicity of infection of 100)12. Each small VRC, initially located at the nuclear periphery, originates from a single viral genome12,13,14,15. These small VRCs undergo actin-mediated directed movement8, resulting in their fusion and eventual enlargement into the entire nucleus. The expansion of the VRCs is accompanied by an increase in the nuclear volume and relocation of the host chromatin into the nuclear periphery16,5. It is in these VRCs that viral nucleocapsid assembly occurs. Soon after assembly, each nucleocapsid penetrates the host chromatin layer and nuclear lamina to the inner nuclear envelope, the site of its egress2,17,18.
Despite their many achievements with biological applications, light- and electron-based imaging techniques suffer from fundamental limitations in 3D imaging of the subcellular architecture of the entire cell. Transmission electron microscopy (TEM) and TEM tomography are limited by fixation-induced distortions of cellular features, damage caused by the electron beam and the limited range of angular sampling. Fluorescence microscopy can determine the positions of specific molecules, but only of those selected based on existing information, including the sizes of the labels and the density of the labelling. Furthermore, immunofluorescence imaging is prone to artefacts by fixation or permeabilization19,20.
We employed a strategy integrating 3D soft X-ray tomography (SXT) imaging with cryogenic fluorescence microscopy (CFM), confocal and electron microscopy, and advanced data analysis in order to study in more detail the HSV-1-induced changes in nuclear architecture, molecular organization of the host chromatin and, in particular, the formation of channels across the chromatin layer. The short wavelength of soft X-rays enabled high-resolution 3D imaging of the nuclear architecture. Moreover, since X-rays can penetrate 15 μm-thick biological material, SXT allows for quantitative assessment of the entire cell. For SXT image acquisition, the cells were placed in (cylindrical) thin-walled capillaries of diameter up to 15 μm. B cells were used because of their small size and HSV-1 susceptibility21,22.
In fact, SXT permits cell imaging in a near-native state i.e. intact, unsliced, unstained, and fully hydrated. X-ray absorption depends on the concentration of organic material in each voxel. Therefore, SXT not only detects multiple cellular structures but can also provide quantitative assessment of their composition and structure. Contrast in soft X-ray microscopy is generated by differential attenuation of X-rays by the biomolecules in the specimen and is not muted by weakly absorbing water. Attenuation of X-rays by the specimen follows the Beer-Lambert law23 and is therefore both a linear and a quantitative measure of the thickness and the chemical species present at each point in the cell24,25,26. To gain insight into the spatial localization of the host chromatin and of the viral and cellular proteins and nucleocapsids, we complemented 3D SXT with confocal and electron microscopy imaging techniques.
Infectivity of B cells
To follow the progress of infection, we analysed the expression of viral immediate-early, early and late genes by cytometry and real-time RT-PCR (qPCR) and of the virus yields by plaque assay27,28,29,30. We detected the presence of viral proteins, as well as the expression of viral lytic genes of all three phases of the replication cycle and substantial production of the progeny virus from the B cells. The highest yield of virus was obtained at 24 h p.i. (Supplementary Figs S1 and S2; see also Supplementary Text S1 for additional information). This suggests that not only could HSV-1 enter and infect B cells but also that its replication cycle was completed. Based on these findings, we decided to use a multiplicity of infection (MOI) of 5 and the time point 24 h p.i. in our subsequent experiments.
Virus-induced reorganization of the host chromatin
To further analyse the nuclear architecture, we used SXT on hydrated cells in their near-native state. The image contrast of SXT is based on the absorption of X-rays by mainly carbon and nitrogen. This allows measurement of the linear absorption coefficients (LAC) of cellular structures reflecting their concentration of cellular biomolecules31,32. Owing to its high density of biomolecules, the heterochromatin region of the nucleus has a er LAC than the less densely packed nucleoplasm, as is evident from computer-generated SXT ortho-slices through nuclei (Supplementary Fig. S3). The use of an HSV-1 strain expressing EYFP-ICP4 allowed the detection of infected cells with enlarged VRC by CFM used in SXT studies. When SXT ortho-slices were aligned with CFM images of the same cell33,34, EYFP-ICP4 was found to be localized in distinct nuclear foci or in a few enlarged foci in the heterochromatin-depleted nuclear regions (Supplementary Fig. S3).
Virus-induced gaps in the cellular chromatin
The distinct LAC values of SXT were used to automatically segment the nuclear structures for 3D visualization of the spatial information in HSV-1 infected cells. Surface-rendered 3D tomographic reconstructions (Fig. 1A; Supplementary Video S1) of the nuclear periphery of the infected cells revealed low-density gaps in the compact layer of marginalized host chromatin. Statistical analysis showed that 24.7 ± 1.2% (n = 7) of the surface area (10 closest voxels with a linear voxel size of 320 nm) of the chromatin had a low-LAC value (0.222–0.322 μm−1), suggesting the presence of low-density regions in the compact chromatin (Fig. 1C). In the non-infected cells the relative area of the region with a low LAC value was 7.5 ± 1.2% (n = 7) (Fig. 1B,C).
In order to further study the low-LAC regions, we analysed their ‘skeletonized’ versions. The skeletonized structure revealed that the low-LAC regions formed channels in the 0.5 μm-thick layer of host chromatin close to the nuclear envelope, some of which penetrated the nuclear periphery across the layer of peripheral (compacted) chromatin in both infected and non-infected cells (Fig. 2A–D: see also Supplementary Video S2). However, in the infected cells the total number of low-LAC breaks, 900 ± 300 (n = 7), was significantly higher (p < 0.01) than in the non-infected cells (170 ± 40, n = 7). Note that the average volume of the nucleus (260 ± 20 μm3), was significantly (p < 0.01) increased in the infected cells compared to the non-infected cells (170 ± 14 μm3). Additionally, analysis of the density of these channels across the layer of marginalized heterochromatin as a function of distance from the nuclear envelope (SXT images) revealed that, in a 0.1 μm band close to the nuclear envelope, the area density of these channels was significantly (p < 0.01) higher in the infected (2.4 ± 0.4 μm−2) than in the non-infected (0.65 ± 0.11 μm−2) cells (Fig. 3A). Furthermore, analysis of their local thickness as a function of distance from the nuclear envelope indicated that their diameter increased towards the nuclear centre, and that they finally merged with the nucleoplasm of the infected (with a VRC) and non-infected cells. The smallest diameter of these channels at the nuclear periphery, at 0.1 μm from the nuclear envelope in both cases, was at least 200 nm (Fig. 3B). Their size was thus sufficient to allow the passage of at least one viral nucleocapsid (diameter 125 nm) at a time.
It is known that heterochromatin-free gaps are associated with NPCs and involved in nucleo-cytoplasmic transport of non-infected cells35. This prompted us to study whether the virus-induced channels are connected with NPCs. The number and distribution of low-DAPI gaps across the heterochromatin were compared with those of NPCs. Immunolabelling of cells with the Nup153 antibody revealed that NPCs frequently formed clusters in both infected and non-infected cells. Some of these clusters were significantly enlarged in the infected cells (Fig. 4A; see also Supplementary Fig. S4). The numbers of NPCs or NPC clusters in the infected cells (105 ± 6, n = 19) were reduced compared to those in non-infected (145 ± 9, n = 20) cells (Fig. 4A,B; see also Supplementary Fig. S4). Accordingly, in the infected cells the total number of low-LAC breaks was higher than the number of NPCs, whereas in the non-infected cells the number of breaks in the nuclear periphery was close to that of NPCs. Moreover, our studies showed that, in the infected cells, the low-DAPI regions were almost always located independently of NPCs, whereas in the non-infected cells, the low-DAPI regions were located adjacent to the NPCs (Fig. 4A,B).
In summary, these results demonstrated that HSV-1 infection increases the number of virus-capsid-sized and NPC-independent gaps, which may facilitate the viral transport across the compacted layer of chromatin.
Nucleocapsids in low-density chromatin gaps
Confocal microscopy was performed to detect the presence of nucleocapsid proteins in the low-density chromatin regions of the nuclear periphery. Viral capsid protein VP5 was frequently seen in low-DAPI regions in close proximity to the nuclear envelope (Fig. 5; see also Supplementary Video S3).
Next, we used transmission electron microscopy to examine the distribution of nucleocapsids with respect to the peripheral marginalized chromatin. The images showed that nucleocapsids were very often localized in the chromatin region next to the nuclear envelope. Consistently with the confocal data, these nucleocapsids were located in the low-density chromatin breaks and narrow virus-nucleocapsid-sized channels (Fig. 6A). The NPCs are mostly invisible in the infected cells, presumably because of virus induced structural changes of the NE. The distribution of chromatin and NPCs in the non-infected cells is shown in the Supplementary Fig. S5. This is in line with recent studies showing that herpesvirus infection increases the porosity of the nucleus, leading to an enhancement of nucleocapsid motility36. Altogether, our findings showed that HSV-1 infection induces breakages penetrating the cellular chromatin barrier to permit nucleocapsid access to the nuclear envelope.
Formation of a VRC as a result of HSV-1 lytic infection is followed by structural changes in the host chromatin5,37,38,39. Profound reorganization of the nuclear chromatin by HSV-1 has been known to include chromatin marginalization to the nuclear periphery16,5. A compact layer of host heterochromatin constitutes an accessibility barrier for the translocation of viral nucleocapsids toward the inner nuclear envelope across which they exit the nucleus. A previous indication of HSV-1 infection’s ability to disrupt marginalized chromatin, so as to allow access through it, has come from immunofluorescence studies showing fragmented distribution of histone H1, suggesting the presence of routes through chromatin5. By combining SXT, CFM and confocal imaging techniques with advanced image analysis, we achieved a new insight into the 3D structure and distribution of such breakages.
We observed that the low-density regions in the host chromatin formed gaps through it. In the absence of infection these openings were typically located near NPCs, in agreement with earlier results on the density distribution of the host chromatin35. In the infected cells, the number of these low-density breakages was significantly increased, and they were most often located independent of NPCs. This independence was in agreement with the NPC-independent nuclear egress of the virus2,40. The SXT analysis revealed channels wide enough to allow the passage of a 125-nm-wide HSV-1 nucleocapsid. However, the narrowest channels, with a diameter of 200 nm, allowed the passage of only one or a few nucleocapsids at a time. This leads to the presence of nucleocapsids in the discontinuous-density regions of the peripheral chromatin close to the nuclear envelope, which agrees well with the egress mechanism demonstrated previously2,5. The exact nature of the molecular mechanisms involved in the intra-nuclear motility of the capsids is controversial. It has been suggested that nucleocapsids are transported in the nucleoplasm via an active process mediated by an intra-nuclear actin and myosin motor protein3,41,42,43,44,45. However, a very recent study showed that their motility is based on passive diffusion36,46.
In summary, we were able to create 3D reconstructions of intact and fully hydrated HSV-1 infected cell nuclei by using SXT. We also used other techniques to complement the structural information thus gained. Our study reveals the induction of channels allowing for the nuclear egress of the progeny viruses across the host chromatin. Moreover, this work used a new combination of methods in the study of virus-cell interactions.
Materials and Method
Cells and viruses
A continuously growing pre-B cell line was produced with the Abelson murine virus47. The female mouse B cells were a gift from Barbara Panning (UCSF School of Medicine, Biochemistry and Biophysics, San Francisco, CA). The Epstein-Barr virus-transformed human B lymphocytes (GM12878) were purchased from the NGIMS Human Genetics Cell Repository, Coriell Institute of Medical Research (NJ). The mouse B and human B cells were maintained as suspension cultures at 37 °C in RPMI-1640 medium (GIBCO, Invitrogen, Inc.), supplemented with 10% or 15% of fetal bovine serum (FBS) (GIBCO, Invitrogen, Inc.), L-glutamine, penicillin, streptomycin and 5% CO2. The medium was refreshed every two to three days to maintain a cell density of 0.8–2 × 106 cells/ml. The HSV-1 strains were wt (17+) and 17+ expressing EYFP-ICP4 (vEYFP-ICP4), a generous gift from R. Everett (MRC Virology Unit, Glasgow, Scotland, UK)1. The viruses had been isolated as described in1. For infection, cells were inoculated with HSV-1 or HSV-1 EYFP-ICP4 (MOI 5–10) and kept at 37 °C until the SXT analysis, live-cell microscopy or fixation.
The cells were infected with HSV-1 or vEYFP-ICP4 HSV-1 at MOI 5. At 24 h p.i., cells were collected by centrifugation (400 RCF for 5 min), spread and air-dried on Zeiss high-performance cover slips (D = 0.17 mm, size 18 × 18 mm2). Cells were fixed with 4% paraformaldehyde (PFA; 20 min at room temperature [RT]). Viral capsid proteins were detected with a capsid protein VP5-specific monoclonal antibody (MAb; Santa Cruz Biotechnology, Inc., TX) and NPCs with Nup153 MAb (Abcam, Cambridge, MA), followed by a goat anti-mouse Alexa 594 secondary antibody (Ab; Molecular Probes, Life Technologies, NY). DNA was stained during embedding with DAPI (4′-6-diamidino-2-phenylindole; Molecular Probes), containing ProLong antifade compound. Imaging was done using a Nikon A1R laser-scanning confocal microscope (Nikon Instruments, Inc., Amsterdam, Netherlands.) with the CFI Plan Apo VC 60XH (NA 1.4, WD 0.13, oil immersion objective). DAPI was excited with a 405-nm diode laser and its fluorescence was detected with a 450/50-nm band-pass filter. EYFP was excited with a 514-nm argon laser and its fluorescence was collected with a 540/30-nm band-pass filter. Alexa 594 was excited with a 561-nm sapphire laser and the fluorescence was collected with a 595/50-nm band-pass filter. For 3D image stacks, 512 × 512 pixels were collected from an appropriate depth depending on the sample. Pixel resolution was adjusted to 50 nm/pixel in x and y dimensions, and to 150 nm in the z dimension. AutoGain was used in multipoint imaging. Iterative deconvolution was performed with a signal-to-noise ratio set at 7, a quality threshold set at 0.0l. Deconvolution was performed with Huygens Essential software (SVI, Hilversum, Netherlands). Image analysis was done with ImageJ48. The number of NPCs was calculated from the maximum intensity projection images. The amount and volume of NPC areas were calculated by a 3D object counter from manually thresholded data. In the fixed-cell images the volume analysis was done by first de-noising the images with a Gaussian blur, sigma = 2, and then making a binary image with the Otsu threshold. Holes were filled and the amount and volume of objects were determined using the 3D-Objects-Counter plugin49.
Specimen preparation for soft X-ray tomography
Cells were seeded at a concentration of 106 cells/ml into a standard plastic culture flask (Corning, Corning, NY), and infected with vEYFP-ICP4 at a MOI of 5 for 24 h at 37 °C. Infected cells were pelleted by centrifugation (400 RCF for 5 min), re-suspended in the growth medium, filtered, loaded into thin-walled glass capillaries and then vitrified by quickly plunging them into liquid-nitrogen-cooled (~90 °K) liquid propane, using a custom device34.
Cryogenic confocal fluorescence microscopy
Vitrified cells expressing EYFP-ICP4 were identified and imaged in specimen capillaries, using a custom-made cryogenic confocal fluorescence microscope. The cryogenic light microscope, made in-house, features a custom-made specimen holder, a modified immersion objective lens and a ~10 l reservoir of liquid nitrogen. Other components were commercial, including a Yokogawa spinning-disk confocal head (CSU-X1, Yokogawa, Tokyo, Japan) and an integrated system of acousto-optical tuneable filtered (AOTF) lasers (Andor Laser Combiner, Model LC-501A; Andor Technologies, Belfast, UK). For more detailed description of the instrument, see34. EYFP was excited using a 491-nm laser and imaged with an EMCCD (iXon Model Numbers DV887ECS-BV, Andor Technologies) using a standard 580/30-nm band-pass emission filter (Chroma Technology Corp., Bellows Falls, VT).
Soft X-ray tomography
SXT data were collected with a soft X-ray microscope XM-2 in the National Center for X-ray Tomography (http://ncxt.lbl.gov) at the Advanced Light Source (http://www.als.lbl.gov) of Lawrence Berkeley National Laboratory (Berkeley, CA) using a 50-nm objective zone plate. Cells were kept in a stream of liquid-nitrogen-cooled helium gas31,50 during data collection, which allowed the collection to be conducted without observable radiation damage. For each data set, 90–180 projection images were collected sequentially around a rotation axis in 1–2° increments, with a total rotation of 180°, using a 300–400 ms exposure time. Projection images were normalized25 and manually aligned using the IMOD software, by tracking fiducial markers on adjacent images51; tomographic reconstructions were calculated using iterative reconstruction methods52,53. LAC values were determined as described54. The student’s t-test (two-tailed, unequal variance) was used to evaluate statistical significance.
Transmission electron microscopy (TEM)
Infected cells and non-infected control cells were fixed in 4% paraformaldehyde and 0.25% glutaraldehyde in 50 mM phosphate buffer (pH 6.8) and post-fixed in 1% OsO4 for 1 h on ice. Cells were dehydrated through an ethanol series and then embedded in low-viscosity embedding resin (TAAB Laboratories Equipment Ltd, UK). Thin sections were cut using Ultracut UC6a ultramicrotome (Leica Mikrosysteme GmbH, Germany) and collected on Pioloform-coated, single-slot copper grids. After double staining with 2% aqueous uranyl acetate and lead citrate, the sections were examined using TEM JEOL JEM1400JEOL (JEOL Ltd., Tokyo, Japan), operated at 80 kV. The images were recorded using a bottom-mounted Quemesa CCD camera with 4008 × 2664 pixel resolution.
How to cite this article: Myllys, M. et al. Herpes simplex virus 1 induces egress channels through marginalized host chromatin. Sci. Rep. 6, 28844; doi: 10.1038/srep28844 (2016).
Everett, R. D., Sourvinos, G. & Orr, A. Recruitment of herpes simplex virus type 1 transcriptional regulatory protein ICP4 into foci juxtaposed to ND10 in live, infected cells. J. Virol. 77, 3680–3689 (2003).
Johnson, D. C. & Baines, J. D. Herpesviruses remodel host membranes for virus egress. Nat. Rev. Microbiol. 9, 382–394 (2011).
Reynolds, A. E., Liang, L. & Baines, J. D. Conformational changes in the nuclear lamina induced by herpes simplex virus type 1 require genes U(L)31 and U(L)34. J. Virol. 78, 5564–5575 (2004).
Salvetti, A. & Greco, A. Viruses and the nucleolus: the fatal attraction. Biochim. Biophys. Acta. 1842, 840–847 (2014).
Simpson-Holley, M., Baines, J. D., Roller, R. & Knipe, D. M. Herpes simplex virus 1 U(L)31 and U(L)34 gene products promote the late maturation of viral replication compartments to the nuclear periphery. J. Virol. 78, 5591–5600 (2004).
Copeland, A. M., Newcomb, W. W. & Brown, J. C. Herpes simplex virus replication: roles of viral proteins and nucleoporins in capsid-nucleus attachment. J. Virol. 83, 1660–1668 (2009).
Pasdeloup, D., Blondel, D., Isidro, A. L. & Rixon, F. J. Herpesvirus capsid association with the nuclear pore complex and viral DNA release involve the nucleoporin CAN/Nup214 and the capsid protein pUL25. J. Virol. 83, 6610–6623 (2009).
Chang, L. et al. Herpesviral replication compartments move and coalesce at nuclear speckles to enhance export of viral late mRNA. Proc. Natl. Acad. Sci. USA 108, E136–44 (2011).
Kobiler, O., Drayman, N., Butin-Israeli, V. & Oppenheim, A. Virus strategies for passing the nuclear envelope barrier. Nucleus 3, 526–539 (2012).
Lukonis, C. J. & Weller, S. K. Formation of herpes simplex virus type 1 replication compartments by transfection: requirements and localization to nuclear domain 10. J. Virol. 71, 2390–2399 (1997).
Simpson-Holley, M., Colgrove, R. C., Nalepa, G., Harper, J. W. & Knipe, D. M. Identification and functional evaluation of cellular and viral factors involved in the alteration of nuclear architecture during herpes simplex virus 1 infection. J. Virol. 79, 12840–12851 (2005).
Kobiler, O., Lipman, Y., Therkelsen, K., Daubechies, I. & Enquist, L. W. Herpesviruses carrying a Brainbow cassette reveal replication and expression of limited numbers of incoming genomes. Nat. Commun. 1, 146 (2010).
Kobiler, O., Brodersen, P., Taylor, M. P., Ludmir, E. B. & Enquist, L. W. Herpesvirus replication compartments originate with single incoming viral genomes. MBio 2, e00278–11 (2011).
Silva, L., Cliffe, A., Chang, L. & Knipe, D. M. 2008. Role for A-type lamins in herpesviral DNA targeting and heterochromatin modulation. PLos Pathog. 4, e1000071 (2008).
Silva, L. et al. Roles of the nuclear lamina in stable nuclear association and assembly of a herpesviral transactivator complex on viral immediate-early genes. MBio 3, e00300–11.
Monier, K., Armas, J. C., Etteldorf, S., Ghazal, P. & Sullivan, K. F. 2000. Annexation of the interchromosomal space during viral infection. Nat. Cell Biol. 2, 661–665 (2008).
Klupp, B. G. et al. Vesicle formation from the nuclear membrane is induced by coexpression of two conserved herpesvirus proteins. Proc. Natl. Acad. Sci. USA 104, 7241–7246 (2007).
Park, R. & Baines, J. D. Herpes simplex virus type 1 infection induces activation and recruitment of protein kinase C to the nuclear membrane and increased phosphorylation of lamin B. J. Virol. 80, 494–504 (2006).
Fischer, A. H., Jacobson, K. A., Rose, J. & Zeller, R. Fixation and permeabilization of cells and tissues. CSH Protoc. pdb top36 (2008).
Wilson, S. M. & Bacic, A. Preparation of plant cells for transmission electron microscopy to optimize immunogold labeling of carbohydrate and protein epitopes. Nat. Protoc. 7, 1716–1727 (2012).
Spear, P. G. & Longnecker, R. Herpesvirus entry: an update. J. Virol. 77, 10179–10185 (2003).
Eling, D. J., Johnson, P. A., Sharma, S., Tufaro, F. & Kipp, T. J. Chronic lymphocytic leukemia B cells are highly sensitive to infection by herpes simplex virus-1 via herpesvirus-entry-mediator A. Gene Ther. 7, 1210–1216 (2000).
Hanssen, E. et al. Soft X-ray microscopy analysis of cell volume and hemoglobin content in erythrocytes infected with asexual and sexual stages of Plasmodium falciparum. J. Struct. Biol. 177, 224–232 (2012).
Larabell, C. A. & Nugent, K. A. Imaging cellular architecture with X-rays. Curr. Opin. Struct. Biol. 20, 623–631 (2012).
Parkinson, D. Y., Knoechel, C., Yang, C., Larabell, C. A. & Le Gros, M. A. Automatic alignment and reconstruction of images for soft X-ray tomography. J. Struct. Biol. 177, 259–266 (2012).
Parkinson, D. Y. et al. Nanoimaging cells using soft X-ray tomography. Methods Mol. Biol. 950, 457–481 (2013).
Nygårdas, M. et al. A herpes simplex virus-derived replicative vector expressing LIF limits experimental demyelinating disease and modulates autoimmunity, PLos One 8, e64200 (2013).
Paavilainen, H. et al. Innate responses to small interfering RNA pools inhibiting herpes simplex virus infection in astrocytoid and epithelial cells. Innate Immun. 21, 349–357 (2015).
Romanovskaya, A. et al. Enzymatically produced pools of canonical and Dicer-substrate siRNA molecules display comparable gene silencing and antiviral activities against herpes simplex virus, PLos One 7, e51019 (2012).
Ziegler, T. et al. Typing of herpes simplex virus isolates with monoclonal antibodies and by nucleic acid spot hybridization. J. Virol. Methods 12, 169–177 (1985).
McDermott, G., Le Gros, M. A., Knoechel, C. G., Uchida, M. & Larabell, C. A. Soft X-ray tomography and cryogenic light microscopy: the cool combination in cellular imaging. Trends Cell Biol. 19, 587–595 (2009).
McDermott, G. et al. Visualizing and quantifying cell phenotype using soft X-ray tomography. Bioessays 34, 320–327 (2012).
Smith, E. A. et al. Correlative cryogenic tomography of cells using light and soft x-rays. Ultramicroscopy 143, 33–40 (2014).
Smith, E. A. et al. The Topological Organization of the Inactive X Chromosome in its Native State. Biophys. J. 106, 434a–435a (2014).
Raices, M. & D’Angelo, M. A. Nuclear pore complex composition: a new regulator of tissue-specific and developmental functions. Nat. Rev. Mol. Cell Biol. 13, 687–699 (2012).
Bosse, J. B. et al. Remodeling nuclear architecture allows efficient transport of herpesvirus capsids by diffusion. Proc Natl Acad Sci. USA 112, E5725–5733 (2015).
Knipe, D. M. & Cliffe, A. Chromatin control of herpes simplex virus lytic and latent infection. Nat. Rev. Microbiol. 6, 211–221 (2008).
Knipe, D. M. et al. M. Snapshots: chromatin control of viral infection. Virology 435, 141–156 (2013).
Weller, S. K. Herpes simplex virus reorganizes the cellular DNA repair and protein quality control machinery. PLos Pathog. 6, e1001105 (2010).
Nagel, C. H. et al. Nuclear egress and envelopment of herpes simplex virus capsids analyzed with dual-color fluorescence HSV1(17+). J. Virol. 82, 3109–3124 (2008).
Tseng, Y., Lee, J. S., Kole, T. P., Jiang, I. & Wirtz, D. Micro-organization and visco-elasticity of the interphase nucleus revealed by particle nanotracking. J. Cell Sci. 117, 2159–2167 (2004).
Cano-Monreal, G. L., Wylie, K. M., Cao, F., Tavis, J. E. & Morrison, L. A. Herpes simplex virus 2 UL13 protein kinase disrupts nuclear lamins. Virology 392, 137–147 (2009).
Forest. T., Barnard, S. & Baines, J. D. Active intranuclear movement of herpesvirus capsids. Nat. Cell Biol. 7, 429–431 (2005).
Leach, N. R. & Roller, R. J. Significance of host cell kinases in herpes simplex virus type 1 egress and lamin-associated protein disassembly from the nuclear lamina. Virology 406, 127–137 (2010).
Roberts, K. L. & Baines, J. D. Actin in herpesvirus infection. Viruses 3, 336–346 (2011).
Bosse, J. B. et al. Nuclear herpesvirus capsid motility is not dependent on F-actin. MBio 7, e01909–14 (2014).
D’Andrea, E., Saggioro, D., Fleissner, E. & Chieco-Bianchi, L. Abelson murine leukemia virus-induced thymic lymphomas: transformation of a primitive lymphoid precursor. J. Natl. Cancer Inst. 79, 189–195 (1987).
Abràmoff, M. D., Magalhães, P. J. & Ram, S. J. Image processing with ImageJ. Biophot. internat. 11, 36–43 (2004).
Bolte, S. & Cordelieres, F. P. A guided tour into subcellular colocalization analysis in light microscopy. J. Microsc. 224, 213–232 (2006).
Le Gros, M. A., McDermott, G. & Larabell, C. A. X-ray tomography of whole cells. Curr. Opin. Struct. Biol. 15, 593–600 (2006).
Kremer, J. R., Mastronarde, D. N. & McIntosh, J. R. Computer visualization of three-dimensional image data using IMOD. J. Struct. Biol. 116, 71–76 (1996).
Mastronarde, D. N. Fiducial marker and hybrid alignment methods for single-and double-axis tomography. In Electron Tomography, 163–185 (Springer, 2006).
Stayman, J. W. & Fessler, J. A. Compensation for nonuniform resolution using penalized-likelihood reconstruction in space-variant imaging systems. IEEE Trans. Med. Imaging 23, 269–284 (2004).
Weiss, D. et al. Tomographic imaging of biological specimens with the cryo transmission X-ray microscope. Nuclear Instruments and Methods In Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 467, 1308–1311 (2001).
We thank the staff of Advanced Light Source (Lawrence Berkeley National Laboratory, Berkeley, CA) staff for providing a safe, reliable source of photons used for imaging the cells in the present study. The SXT research was conducted at the National Center for X-ray Tomography (NCXT), and first-class experimental support by Rosanne Boudreau is gratefully acknowledged. We thank Liisa Lund, Arja Strandell, Mervi Lindman and Mervi Laanti for technical assistance and Electron Microscopy Unit of the Institute of Biotechnology, University of Helsinki, for EM sample preparation. We are grateful to Klaus Hedman for comments on the manuscript. This work was supported by INSERM, CNRS, Université Claude Bernard Lyon-1 and Ecole Normale Supérieure de Lyon (AS). It was financed by the Jane and Aatos Erkko Foundation (JT, MVR), the National Institute of General Medical Sciences of the National Institute of Health, under the award number P41 GM103445, and the US Department of Energy, Biological and Environmental Research (DE-AC02-05CH11231, CAL) and the Academy of Finland, under the award numbers 138388 (MVR) and 259725 (VH).
The authors declare no competing financial interests.
About this article
Cite this article
Myllys, M., Ruokolainen, V., Aho, V. et al. Herpes simplex virus 1 induces egress channels through marginalized host chromatin. Sci Rep 6, 28844 (2016). https://doi.org/10.1038/srep28844
Current Clinical Microbiology Reports (2019)
Scientific Reports (2017)