Figure 3 : LDLR is expressed on lymphatic endothelial cells of collecting lymphatic vessel and its level is modulated by PCSK9.

From: Effects of LDL Receptor Modulation on Lymphatic Function

Figure 3

(a) LDLR expression on smooth muscle cells (smooth muscle actin, SMA) and LEC was detected by immunofluorescence of DAPI, podoplanin (PODO) and LDLR in longitudinally imaged single plan in popliteal lymphatic collecting vessels of WT mice. In the lower right panel, z-stacks were acquired and deconvolved. (b) Immunofluorescence of longitudinally imaged lymphatic collecting vessel single plan of WT, Pcsk9−/− and Ldlr−/−; hApoB100+/+ animals. Images were acquired with an LSM 710 Confocal Microscope (Zeiss) equipped with a 63×/1.4 oil dic objective. (c) Popliteal lymphatic collecting vessels were isolated and digested. LDLR protein expression was assessed by Western Blot in WT, Pcsk9−/− and Ldlr−/−; hApoB+/+ mice. LDLR protein was observed at 160 kDa. The table on the right confirms equal protein loading.