Morphology and immunofluorescence staining of undifferentiated hNPCs ((A–C): a neuroshpere) into PPRF-h2 showed that the majority of the cells express mainly (A) Nestin⁺ cells, which evidences their progenitor profile. hBM-MSCs CM collected from static and dynamic culture conditions was able to significantly increase the survival and differentiation of hNPCs into (E,F) immature (DCX⁺ cells) and (I,J,M,N) mature (Map-2⁺/NeuN⁺ cells) neurons when compared to the (D,H,L) control group ((G,K,O); mean ± SEM., n = 3, p < 0.001). At the same time, the (F,J,N) hBM-MSCs CM collected from the dynamic culture conditions also showed increased levels of DCX⁺ (p < 0.05) and MAP-2⁺ (p < 0.05) cells, and NeuN⁺ (p = 0.077) cells compared to the (E,I,M) static hBM-MSCs CM in the induction hNPC differentiation ((G,K,O); mean ± SEM., n = 3). qRT-PCR analysis to NOTCH1 on hNPCs-differentiated cells showed a higher expression (p < 0.05; mean ± SEM; n = 3) in the (P) hBM-MSCs dynamic secretome group when compared to the control group. CT: Control (Neurobasal-A media), CMs: hBM-MSCs static conditioned media. CMd: hBM-MSCs dynamic conditioned media (Scale bar: 50 μm). Data are expressed as mean ± SEM. *p<0.05; **p<0.01; ***p<0.001.