Genetic distances were calculated by the Kimura 2-parameter substitution model and phylogenetic analyses by applying the neighbor-joining method as described for CSFV phylogeny33. Phylogenetic analysis of partial NS2-3 encoding APPV sequences (1581 nt) from three different regions in Germany (bold) together with respective sequences of established and tentative pestivirus species were analyzed. The GenBank accession number and the corresponding host species (symbols) are indicated for each individual sequence. APPV sequences from Lower Saxony [KU041637] and from Bavaria [KU041639] were obtained from apparently healthy adult pigs. APPV sequence from North Rhine Westphalia [KU041638] was obtained from a congenital tremor diseased piglet (highlighted in red). Bootstrap values were calculated for 1000 iterations. Only significant bootstrap values (≥700) of major nodes are given in the tree. Trees were displayed by dendroscope38.