Honey bee, Apis spp., colonies can be regarded as superorganisms, in which cooperating individuals in overlapping generations support functions comparable to those of cells in a multicellular organism1. Such cooperation can foster the survival of parasite-infested colonies via social immunity2,3, e.g. social analogues of encapsulation4 or fever5. However, social immunity might be inefficient if parasites shift host species such as in the case of the ectoparasitic mite Varroa destructor, which originally infested the Eastern honey bee A. cerana and now infests the Western honey bee, A. mellifera, at a global scale6. There is general consensus that this ubiquitous mite, together with the viruses it vectors6, is the main biotic factor threatening A. mellifera colony survival, because infestations usually result in colony death within 2–3 years6,7,8,9. This is due to the exponential growth of mite populations sustained by developing worker brood throughout the year and seasonal male brood6. In sharp contrast, infested colonies of the original host, A. cerana, are surviving. Among several potential resistance traits of A. cerana6, the apparent absence of mite population growth in association with worker brood is likely to be most significant10. Other A. cerana populations naturally infested with different mite species and haplotypes also appear resistant to the ubiquitous invasive V. destructor Korean haplotype infesting sympatric A. mellifera in Asia11. Social immunity2 is likely to play a major role as resistance mechanism; for instance, adult honey bee workers cooperate to detect and remove worker brood infested by this mite, thereby interrupting its reproduction6,12. The trigger of this hygienic behaviour by workers is based on signals from infested brood13 and/or by cues from the parasite14, on the ability of workers to detect this information as well as on their response thresholds11,15,16. The ability of A. cerana workers to perform hygienic behaviour towards V. destructor-infested brood is greater than that of the new host, A. mellifera17. However, the mechanisms underlying this major resistance trait of A. cerana are unknown. In particular, the crucial role of mite-infested brood in inducing this behaviour has not yet been fully investigated, since all previous studies have been performed in the presence of adult workers, thereby confounding the respective roles of signals/cues, of detection abilities and of response thresholds, which may all contribute to efficient hygienic brood removal.

Here, we took an alternative approach to address the apparent gaps in our understanding of the mechanisms underlying the principal resistance traits of the original host of V. destructor, A. cerana. The traditional approach to dealing with complexity is to reduce or constrain it. Here, we experimentally reduce the complexity of our system by excluding one level (adult workers), thereby allowing for the first time to distinguish between the effects of brood and adult workers in the hygienic behaviour. By monitoring the development and survival of mite-infested honey bee brood of A. cerana and A. mellifera colonies in both the absence and presence of adult workers, we separated the respective roles of the developing brood from that of adult nestmates in the resulting hygienic behaviour. We hypothesised that the brood of A. cerana is more susceptible compared to A. mellifera, thereby providing the basis for a more efficient removal behaviour by adult nestmates. A higher susceptibility of infested or wounded worker brood associated with efficient hygienic behaviour in adult workers is expected to reinforce the social immunity of a honey bee colony infested by brood parasites or pathogens and may contribute to colony survival.

Results and Discussion

We infested 314 worker larvae from 6 colonies in one population of A. mellifera and from 4–5 colonies in each of three distant populations of A. cerana. For infestations, we used the invasive Korean haplotype of V. destructor18. Wax combs containing infested and non-infested control brood cells were placed in an incubator mimicking hive conditions for optimal brood development19. The cells were opened one day prior to the expected emergence date of the adult bees and the developmental stages of the individuals were recorded20.

The data shows a striking difference in the effect of parasitism between A. cerana and A. mellifera. The development of A. mellifera worker brood infested by V. destructor was similar to the non-infested controls. In contrast, in the three A. cerana populations, we found a higher frequency of individuals at early developmental stages in infested worker brood than in control brood (Fig. 1; Supplementary Fig. S1). Overall, the development of infested individuals was significantly delayed in A. cerana compared to A. mellifera (one-way ANOVA on log-transformed values with a Dunnett’s post hoc test using A. mellifera as control group, df = 3, F = 7.2, p = 0.003, Fig. 2). Whenever we observed larvae or pre-pupae (earliest developmental stages) one day prior to expected emergence, most of them were decomposed and were obviously dead (Fig. 1).

Figure 1
figure 1

Mite-infested brood (above row) and control brood (bottom row) from an A. cerana colony (Phatthalung, Thailand).

Individuals were removed from their cells one day before emergence was expected. Most infested individuals stopped development at the larval and prepupal stages and died, Photo by Paul Page.

Figure 2
figure 2

Delay in brood development calculated as the difference in number of developmental stages (x axis) separating infested brood from non-infested (control) brood in three populations of A. cerana and one of A. mellifera (y axis).

The average developmental delays within were compared by using a one-way ANOVA on log-transformed values combined with a Dunnett’s post hoc test using A. mellifera as control group. Values represented are means ± 1 S.E.M. ***P < 0.001.

In the presence of workers and even when assuming an equal ability of both honey bee species to detect signals triggering hygienic behaviour as well as equal response thresholds towards them, this observed difference in brood susceptibility is likely to elicit an earlier and more frequent production of such signals, ultimately leading to more efficient removal behaviour and overall enhanced social immunity. In order to test this hypothesis, we subjected larvae of both species to a benign wounding and either placed them in an incubator to monitor their development or back into their colony of origin. A. cerana brood died more quickly and in greater proportions than A. mellifera in incubator conditions (Log-rank Mantel test, df = 1, Chi2 = 12.8, p < 0.001, Fig. 3a; Supplementary Fig. S2) and in colonies, A. cerana workers removed the wounded brood significantly more and faster than A. mellifera (Log-rank Mantel test, df = 1, Chi2 = 67.8, p < 0.001, Fig. 3b). These results indicate that A. cerana brood was affected faster and to a higher degree by wounding than A. mellifera and that wounded brood could be detected by workers for a more efficient hygienic removal. The higher proportion of brood removed by the workers in the colonies compared to our estimate of brood death in the incubator (Fig. 3) is likely due to the sensitivity of workers to dysfunctional individuals that could have survived in incubator conditions. Nonetheless, a benign wounding of A. cerana larvae appears to be sufficient to yield a reaction analogous to apoptosis in multicellular organisms. Given the use of a sterile pin for the pricking, these results also allow the exclusion of secondary pathogens commonly carried by V. destructor6 in the mortality observed.

Figure 3
figure 3

(a) Mortality of wounded brood in A. cerana and A. mellifera developing in an incubator. The survival status of larvae pricked with a sterile glass-pulled needle and of control larvae was monitored every 12 hours during three days; (b) Removal of wounded brood in A. cerana and A. mellifera colonies. Larvae pricked with a sterile glass-pulled needle and controls were exposed to workers and their removal via hygienic behaviour was monitored every 12 hours during three days. Brood mortality and removal rates were compared with log-rank Mantel-tests. Values are means ± 1 S.E.M. **P < 0.01.

Hence, the significantly higher susceptibility of mite-infested brood of the original host of V. destructor leads to more efficient hygienic behaviour, thereby providing a basis for honey bee colony survival to parasitism and constituting an additional resistance trait of the original host of this parasite. Our results provide a most parsimonious explanation for the striking differences in the impact of infestations by the invasive Korean haplotype of V. destructor mites between the Western and the Eastern honey bee species. Brood susceptibility could also contribute to colony survival to V. destructor mite infestations in naturally resistant A. mellifera populations6,21,22,23,24.

Our counterintuitive result shows that susceptible individuals can benefit the superorganism, which goes against the common assumption that ‘strong’ elements of a social entity are required to ensure group survival. We here show that ‘weak’ elements can contribute to the strength of a social ensemble. This provides empirical support to theories on the adaptive value of suicide or high death rate in social insects25,26, thereby constituting a social analogue of apoptosis, a mechanism preventing the spread of infections by sacrificing parts of the whole organism27,28. Given the high number of individuals in a honey bee colony, some individuals can be (and are commonly) sacrificed without compromising the survival of the group29 and even promote colony survival by hindering further infestations30. Such altruistic suicide of immature individuals is a novel form of transgenerational social immunity in honey bees. Irrespective of the actual pathogen, the observed social apoptosis is most likely a fundamental defence mechanism of social insect colonies to combat diseases.

Mitigating the elevated losses of managed Western honey bee colonies, A. mellifera, experienced globally8 is crucial to ensure the sustainability of their valuable pollination services towards agriculture31 and ecosystem functioning32. If A. mellifera populations are to show variation in brood damage linked to V. destructor infestation or other diseases, a breeding concept based on brood susceptibility could be implemented. This might increase the efficiency of breeding programs and thus help alleviate the global losses of managed honey bees due to this parasitic mite and other brood diseases.

Materials and Methods

Infestation experiments

Mite collections and honey bee colonies

Adult V. destructor female mites were collected from drone and worker brood of A. mellifera colonies. We used the same colonies as mite sources to infest both species at each location. Batches of 10–20 collected mites were kept on 15–20 A. mellifera adult workers in plastic cages for at least two days, thereby mimicking the wandering stage of mites prior to reproduction in honey bee brood cells6. The invasive K1 (Korean) V. destructor haplotype was confirmed on a mite subsample (N = 146) using standard methods20 by comparing mitochondrial DNA sequences to references deposited in GenBank (V. destructor Cox-1 gene 458 bp fragment; Korean haplotype, accession number AF106899.1)18. Infestation experiments were performed in six colonies of A. mellifera in Chiang Mai (Northern Thailand; N 18°48′13″ and E 98°57′22″, A. mellifera can only survive in the northern part of the country) and on five, five and four colonies of A. cerana in Chiang Mai, Phatthalung (Southern Peninsular Thailand; Khuan Khanun, N 7°44′57″ and E 100°00′03″) and Samui island (N 9°32′05″ and E 99°58′28″), respectively.

Brood comb preparation and infestations

V. destructor mites reproduce on honey bee brood developing within the wax cells of host nests. They enter these cells just before they are sealed with a wax cap by workers6. In A. mellifera combs, we mapped the positions of cells containing uncapped larvae at the appropriate stage (L5 larvae) on transparent sheets and reintroduced the comb into its colony of origin for natural capping by the workers to occur. Experimental cells were then selected among those that were capped within 6 hours and subsequently used for infestations20. Such infestations do not limit mite reproduction33,34. We partially opened the capping of selected cells using a razor blade edge and assigned one of two treatments: (1) infested cells: a single adult V. destructor female mite was introduced into the cell by using a fine paint brush and (2) control cells: the opening was only lightly brushed without introducing a mite. In both cases, the cell capping was then resealed.

In A. cerana, natural wax combs are not built on wooden frames so they cannot be placed back into their colony of origin to be capped naturally. Thus, the cells used for these experiments were identified based on a visual assessment of larval developmental stage and when possible of partial capping of the cells. Infestation (1) or sham treatment (2) was performed as above for A. mellifera, but the cells were capped with a layer of paper tissue. This layer of paper was sealed to the cell walls with the use of a pre-heated nail head.

For each colony, we used approximately the same number of infested and control cells, which varied from 8–45 and 9–46 respectively, depending on the number of L5 larvae available at the beginning of each infestation experiment. Once the infestations were performed, we cut out the portion of comb with the infested cells and suspended it in an incubator at developing brood conditions, i.e. 34.5 °C and 70–90% RH. Whenever field conditions did not allow us an access to a laboratory incubator, we used a portable incubator connected to a 12 V battery and yielding identical temperature and humidity conditions in order to store the combs. Apis cerana and A. mellifera workers display the same developmental periods before capping: 3 days from egg to larva followed by 6 days of molting larval instars (L1 to L5). After capping occurs, however, their preimaginal stages differ by one day, as A. mellifera workers need 12 days of pupation to fully develop into an adult, while A. cerana workers only require 11 days until bee emergence35. We thus opened the experimental cells one day before the expected emergence date and reported the developmental stage of the opened brood as well as its survival status. We recorded the developmental data as follows: larva, pre-pupa, white-eyed, pink-eyed and purple-eyed pupae were considered to be immature stages of development (stages 1–5), whereas yellow-thorax, grey-winged, grey-thorax, grey-abdomen pupae and adults were defined as mature stage (stage 6) based on the developmental pattern observed in the control at the time of cell opening.

Data and statistical analyses

We used data from cells containing brood that was not affected by chalkbrood infection, by wax moths or by involuntary crushing of the comb. In addition, we only considered artificially infested cells that showed a clear sign of infestation, i.e. the occurrence of feces or the presence of a V. destructor mite in the cell and that were not naturally infested by either Tropilaelaps spp. or local Varroa spp. mites, prior to the experiment.

We ranked each individual from 1 (larva) to 6 (mature), depending on its developmental stage at cell opening. Within each replicate, we calculated an average developmental stage for infested and control cells separately and subtracted the former from the latter, in order to obtain a mean developmental delay per colony. All residuals were normally distributed within populations and the variance was homogeneous between populations. We then compared the average developmental delays within each species and each location by using a one-way ANOVA on log-transformed values combined with a Dunnett’s post hoc test and A. mellifera as the control group. Statistical calculations were performed using Systat software (version 13).

Further analyses were performed with generalized linear models (GLMs) applied to the same developmental stage dataset with colony as random factor and using the lmer function from the package lme436. Response variables were the frequencies of control and infested individuals at each stage in each colony. They were modelled with Poisson error distributions. Statistical tests were performed in R software (R version 3.2.2)37 and are summarized in the Supplementary Table S1.

Larvae wounding experiments

Honey bee colonies and brood comb preparations

We used 5 colonies of Apis mellifera and 5 of Apis cerana kept in Langstroth hives in an apiary at Zhejiang University, Hangzhou (China) for the larval wounding experiments. On the combs of both species, we identified cells by mapping brood at the L5 stage, as described above. We then replaced the comb into the colony for capping by the workers to occur and identified the capped cells 6 hours after the mapping. We used a fine pulled-glass capillary with a diameter similar to that of the V. destructor mite chelicerae (Ø = 50 μm) to wound the larvae by pricking, simulating the wound induced by V. destructor mites feeding on the bee larvae38,39. We inflicted wounding by pressing the tip of the capillary against the cuticle until it gave way. We sterilized the needle with ethanol before each use and replaced it anew for each colony tested. Two experiments were conducted in each species in order to quantify the susceptibility of larvae towards wounding, firstly in the absence of workers and secondly in their presence to compare the removal of wounded brood by workers, i.e. hygienic behaviour.

(1) To measure the susceptibility of wounded larvae, we pricked 30 freshly capped larvae and instead of resealing the wax capping, we removed it and closed the cell with a transparent gelatin cap, allowing for the observation of brood development40. As a control, we opened 15 freshly capped cells and sealed them with a gelatin cap without pricking the larvae (controls for the effect of pricking) and we left 15 cells non-manipulated (controls for the effect of gelatin cap on larva survival). We then placed the comb into an incubator at developing brood conditions, i.e. 34.5 °C and 70% RH and checked the experimental cells every 12 hours during the next three days. At every check, we reported the survival status of the brood in the cells. We considered a larva dead when it turned black and deflated or when it did not stretch in the cell as is typical for the transition to pre-pupal stage. We opened the non-manipulated cells after 72 hours to identify the final state of larvae/pre-pupae.

(2) To measure the hygienic removal of wounded larvae, we opened 30 freshly capped cells and gently pricked the larvae inside of them. As a control, we opened 15 cells without pricking the larvae (positive controls). Wax caps of cells with pricked and non-pricked larvae were then resealed. As a control for the effect of cell opening on removal by workers, we mapped an additional 15 cells that we did not manipulate (negative controls). The comb was then replaced into its original colony and the experimental cells were checked every 12 hours during the next 3 days. At every comb check, we reported the number of mapped brood cells that had been cleaned out by the workers of each species during an interval, i.e. brood removal.

Data and statistical analyses

(1) To assess susceptibility to wounding, we counted the dead and surviving larvae in each species and at each time interval. (2) To compare hygienic removal of wounded brood, we counted the number of wounded brood removed by the workers of each species at each time interval. As the respective mortality and removal rates of the different controls were not significantly different for either experiment (log-rank Mantel test; susceptibility: A. mellifera, df = 1, Chi2 = 0.34, p = 0.56; A. cerana, df = 1, Chi2 = 0.0, p = 0.99; hygienic removal: A. mellifera, df = 1, Chi2 = 2.0, p = 0.16; A. cerana, df = 1, Chi2 = 1.0, p = 0.32), these data were pooled. The validity of our assays was confirmed by the control mortality being below a 15% threshold41. We then compared the mortality rates and wounded brood removal rates between bee species by means of log-rank Mantel tests.

Additional Information

How to cite this article: Page, P. et al. Social apoptosis in honey bee superorganisms. Sci. Rep. 6, 27210; doi: 10.1038/srep27210 (2016).