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Mice were divided into the following six groups: control mice (CTR); wheel-running mice (WR); tumor-bearing mice (C26); tumor-bearing, running mice (C26 WR); tumor-bearing, AICAR-treated mice (C26 AICAR); tumor-bearing, rapamycin-treated mice (C26 RAPA). All the treatments lasted 19 days, as explained in detail in the Methods, and the initial and final body weight were measured (the final weight of C26-bearing mice was equal to the carcass weight, i.e. total body weight minus tumor weight). Two-way ANOVA for repeated measures revealed no effect of WR on the body weight of healthy mice (control) but a significant effect of the presence of the tumor on the body weight of C26-bearing mice. In addition, ANOVA showed that the treatments had a significant effect in rescuing body weight. Multiple comparisons by Tukey’s HSD tests were used as post-hoc tests: **p < 0.0005 vs initial; *p = 0.001 vs initial; ##p < 0.0005 vs C26 final; #p = 0.001 vs C26 final. C26 WR is NS vs C26 AICAR or C26 RAPA. The means ± SEM are shown; 9 < n < 34 for each group. The results show that each treatment significantly counteracts the C26-induced body weight loss.