Figure 2 : Wheel running counteracts tumor-induced atrogene expression and restores the autophagic marker levels, which are altered in both murine and human skeletal muscle in cancer cachexia.

From: Aerobic Exercise and Pharmacological Treatments Counteract Cachexia by Modulating Autophagy in Colon Cancer

Figure 2

(a) Expression of Atrogin 1 and MuRF1 genes by real-time RT-PCR, in mice in the absence (—) or presence (WR) of wheel running, 19 days after transplantation in C26-bearing mice and in control mice (CTR). Values were normalized to Gapdh expression. Data are presented as mean ± SEM. Kruskal-Wallis test revealed a global significant difference between the three groups, followed by post-hoc test *p < 0.05. (b) Representative immunostaining for p62 on TA muscle of C26-bearing mice in the absence (—) or presence (WR) of wheel running for 19 days, showing an increase of p62 induction in C26-bearing mice. Scale bar = 20 microns. (c) Western blot analysis of LC3bI, LC3bII and p62 expression in control mice (CTR), or mice in the absence (—) or presence (WR) of wheel running, 19 days after C26 transplantation. Gapdh was used as a loading control. Densitometric analyses of Western blot showing LC3bII/LC3bI ratio (d) and p62/Gapdh (e) ratio in mice in the absence (—) or presence (WR) of wheel running, 19 days after C26 transplantation, compared with control mice (CTR). Data are presented as mean ± SEM. F = 4.63; df 2; p < 0.05 and F = 14.18; df 2; p < 0.02 by ANOVA; *p < 0.005 and **p < 0.005 by Tukey’s HSD test. (f) Expression of LC3b and p62 by Western blot analyses in skeletal muscle biopsies from colon carcinoma patients and healthy (CTR) subjects. Gapdh was used as a loading control. The lanes were run on the same type of gel but were non-contiguous, as indicated by the thin black lines. Densitometric analyses of Western blot showing significantly higher expression of LC3bII/LC3BI ratio (g) and p62/Gapdh ratio (h) in skeletal muscle biopsies from colon carcinoma patients (CARC), compared with healthy subjects (CTR). Data are presented as mean ± SEM. *p < 0.05 by Student’s t-test.