Abnormal intrinsic dynamics of dendritic spines in a fragile X syndrome mouse model in vivo

Dendritic spine generation and elimination play an important role in learning and memory, the dynamics of which have been examined within the neocortex in vivo. Spine turnover has also been detected in the absence of specific learning tasks, and is frequently exaggerated in animal models of autistic spectrum disorder (ASD). The present study aimed to examine whether the baseline rate of spine turnover was activity-dependent. This was achieved using a microfluidic brain interface and open-dura surgery, with the goal of abolishing neuronal Ca2+ signaling in the visual cortex of wild-type mice and rodent models of fragile X syndrome (Fmr1 knockout [KO]). In wild-type and Fmr1 KO mice, the majority of baseline turnover was found to be activity-independent. Accordingly, the application of matrix metalloproteinase-9 inhibitors selectively restored the abnormal spine dynamics observed in Fmr1 KO mice, without affecting the intrinsic dynamics of spine turnover in wild-type mice. Such findings indicate that the baseline turnover of dendritic spines is mediated by activity-independent intrinsic dynamics. Furthermore, these results suggest that the targeting of abnormal intrinsic dynamics might pose a novel therapy for ASD.


Results
Chronic infusion of the adult brain using a microfluidic device. The influence of activity on basal spine turnover was investigated using a brain interface device 25 that enabled the infusion of Ca 2+ inhibitors into Typical Ca 2+ transients obtained from mice infused with either ACSF (d,e; traces from ROIs in (c)), APV (g,h), or APV+ iVDCC (k,l) and mice who received MK801 intraperitoneally (i,j). Red traces represent those from spines, and black traces from the dendritic shafts. Dashed areas in (d,g,i,k) are magnified in (e,h,j,l), respectively. Blue bars in (e,h,j) indicate dendritic Ca 2+ transients and the red bar in e indicates spine Ca 2+ transients. (f) The percentage of the three patterns of Ca 2+ transients that contain both spine and dendritic transients (open), only dendritic transients (blue), and no transients (black). The patterns were distinguished based on 4-min images of all spines along an imaged dendrite. Data were obtained for the infusion of ACSF (11 dendrites, 10 cells, four mice, 134 spines,), APV (15 dendrites, 11 cells, four mice, 124 spines), or APV+ iVDCC (11 dendrites, nine cells, four mice, 83 spines), and for the injection of MK801 (16 dendrites, 15 cells, five mice, 117 spines). The number on each bar indicates the number of dendrites classified. Fisher exact test, p < 0.0001. the visual cortex in adult mice (2-6 months old) (Fig. 1a,b). With regard to the surgical method, 20% mannitol was administered to allow the detachment and removal of the dura without directly touching the brain. To maintain a clear cranial window after open-dura surgery, the dural blood vessels were coagulated to prevent bleeding prior to the removal of the dura mater ( Supplementary Fig. 1a-c). Two-photon imaging was performed 1 day post-surgery, and chronic infusion was initiated immediately after the first imaging session, to avoid clogging of the inlet of device, using an osmotic pump implanted on the backs of mice (Fig. 1a).
When infusing Alexa594 at the cortical surface, the dilution factor was estimated to be 4% of the original solution in the osmotic pump ( Supplementary Fig. 1f-i). The concentration profile of Alexa594 was evaluated across the depth of the cortex, where analysis determined that the concentration did not significantly differ among the cortical layers ( Supplementary Fig. 1h,i). Hydrophilic drugs were selected for the infusion experiment, as they diffuse effectively into the brain 26,27 .
Under these conditions, no signs of inflammation were detected with regard to microglia or astrocyte activity. In vivo imaging of ionized calcium binding adaptor molecule 1 (IBA1)-positive microglia on days 1 and 3 demonstrated relatively limited microglial migration while their processes were dynamic 28 ( Supplementary  Fig. 2a). Immunostained slices from the brains of artificial cerebrospinal fluid (ACSF)-infused mice indicated similar levels of IBA1-positive microglia with regard to the surgical and contralateral sides. Comparatively, in positive controls where the cortex was gently depressed during surgery, the number of immunoreactive cells doubled ( Supplementary Fig. 2b-d). Slice thickness did not affect the results (IBA1-positive cell ratio of 100 or 50 μ m slices, 1.05 ± 0.06 and 0.99 ± 0.05, respectively; glial fibrillary acidic protein (GFAP)-positive cell ratio of 100 or 50 μ m slices, 1.03 ± 0.05 and 1.04 ± 0.08, respectively). The number of IBA1-positive microglia did not significantly differ between layers I and II/III ( Supplementary Fig. 2e). Similarly, the number of GFAP-positive astrocytes did not significantly differ between the surgical and contralateral sides, and significantly less than in positive controls (Supplementary Fig. 2f-h). The number of GFAP-positive astrocytes was higher in superficial layer I than in layers II/III on both the surgical and contralateral sides ( Supplementary Fig. 2i), in line with previous findings 29 .
Intrinsic dynamics of dendritic spines in wild-type mice. The first images of the dendritic spines of wild-type (Thy1-GFP M-line) mice were obtained immediately prior to infusion, subsequent to which, images were obtained at every 2-day interval (Fig. 2a). In this and ensuing experiments (Figs 2 and 3), all 2-day interval data were combined (Fig. 2a), as no significant differences were identified between data obtained from the first and subsequent imaging intervals ( Supplementary Fig. 3). The rates of spine generation and elimination did not significantly differ between mice fitted with conventional glass windows or microfluidic devices (Fig. 2c,d). The efficacy of various inhibitors was assessed with regard to activity-dependent spine remodeling in mice reared in visually enriched environmental (EE) conditions (Fig. 2b,e). Accordingly, the rates of spine generation were relatively high in the EE condition ( Fig. 2e,g) compared to the normal condition (NC) (see Fig. 2c,k). In addition, the rate of spine generation was markedly suppressed by infusion of either APV or APV+ iVDCC (Fig. 2f,g), indicating that NMDA receptors play an important role in spine structural plasticity. Similarly, the rates of spine elimination were equally suppressed by the administration of inhibitors (Fig. 2h). The same results were obtained following the intraperitoneal application of MK801 (Fig. 2g,h).
However, spine turnover persisted in the presence of various inhibitors (Fig. 2g,h), which was confirmed in mice reared in under NC (Fig. 2b,i-l). Moreover, the spine generation rate was unaffected by the infusion of APV, APV+ iVDCC, APV+ iVDCC+ TTX, or intraperitoneal injection of MK801 when compared to ACSF superfusion (Fig. 2k). The spine elimination rate was slightly reduced by inhibitor administration (Fig. 2l), indicating that this marginally reflected baseline learning in the NC group.
Abnormal intrinsic dynamics of dendritic spines in Fmr1 KO mice. The baseline rate of spine turnover was greater in Fmr1 KO than in wild-type mice, and was not significantly enhanced by environmental enrichment (Fig. 3). This finding was consistent with previous results 13,20 . Increased spine turnover was unaffected by infusion of APV+ iVDCC or intraperitoneal MK801 ( Fig. 3b-d,f).
Next, the effects of GM6001 dose were investigated with regard to intrinsic turnover. In wild-type mice, GM6001, an MMP9 inhibitor, was found to block EE-induced spine generation when administered at high concentrations (20 and 50 mg/kg), but produced no effect at lower concentrations (5 and 10 mg/kg) (Figs 2g,h and 4). This is consistent with the blockade of long-term potentiation identified in previous studies 23,24,31 . In Fmr1 KO mice, a low concentration of GM6001 (10 mg/kg) almost completely abrogated excess baseline turnover (Fig. 3c,e,f), while no effect was identified in wild-type mice (Fig. 2k,l). Similar rates of turnover were seen even at high concentrations of GM6001 (20 and 50 mg/kg) in EE mice (Fig. 4). This indicates that normal intrinsic dynamics were unaffected by the application of MMP9 inhibitors in wild-type mice, whereas GM6001 inhibited the abnormal intrinsic dynamics observed in Fmr1 KO mice. Similar results were obtained with regard to spine generation following the administration of minocycline (Figs 2g,h and 3c,f), which inhibits the synthesis of MMP9 32 .  (e,f,i,j) Examples of dendritic branches from mice reared in EE conditions and superfused with ACSF (e) or APV (f), and in mice reared in NC and superfused with ACSF (i) or APV (j). Spines that were eliminated (blue arrowheads) or generated (red arrowheads) are indicated. (g,h,k,l) Spine generation (g,k) and elimination (h,l) rates for mice reared in EE (g,h) or NC (k,l) with the device. Mice received superfusion of ACSF (15 intervals, nine cells, seven mice, 2021 spines from EE; 13 intervals, five cells, five mice, 3154 spines from NC), APV (14 intervals, seven cells, five mice, 1241 spines from EE; 13 intervals, five cells, five mice, 1767 spines from NC), APV+ iVDCC (11 intervals, five cells, five mice, 815 spines from EE; 11 intervals, five cells, five mice, 1442 spines from NC), or APV+ iVDCC+ TTX (10 intervals, five cells, five mice, 1042 spines from NC). Alternatively, mice underwent open-skull surgery with an intraperitoneal injection of MK801 (12 intervals, five cells, five mice, 1412 spines from EE; 10 intervals, five cells, four mice, 1520 spines from NC), GM6001 (10 mg/kg; nine intervals, five cells, three mice, 823 spines from EE; nine intervals, five cells, four mice, 1112 spines from NC), or minocycline treatment (nine intervals, five cells, four mice, 820 spines from EE). Numbers on each bar in (c,d,g,h,k,l) indicate the intervals analyzed. Mann-Whitney test, p = 0.9735 in c and p = 0.7762 in (d). Kruskal-Wallis test, p < 0.05 in (g,h), p = 0.9288 in (k) and p < 0.01 in (l). * p < 0.05, * * p < 0.01 using a Steel's test with respect to ACSF (g,h,l).  (c,f) Spine generation (c) and elimination (f) rates over 2-day intervals in wild-type or KO mice with a glass window reared in NC (wild-type: 12 intervals, five cells, five mice, 1526 spines; KO: 14 intervals, six cells, five mice, 2393 spines), KO mice raised in EE conditions (nine intervals, five cells, five mice, 1983 spines), KO mice that received intraperitoneal MK801 (10 intervals, five cells, four mice, 1233 spines), GM6001 (10 intervals, five cells, three mice, 1141 spines), or minocycline through drinking water (eight intervals, five cells, five mice, 1090 spines), in addition to mice that received the interface device with infusion of APV+ iVDCC (eight intervals, five cells, three mice, 996 spines). The numbers on each bar in c and f indicate the number of intervals analyzed. Spine generation and elimination rates between each imaging session were averaged within each group. Kruskal-Wallis test, p < 0.01 in (c,f). * p < 0.05, * * p < 0.01 using a Steel's test with respect to KO mice that received a glass window.

Discussion
The present study investigated the activity-independent intrinsic dynamics of dendritic spine turnover via the implantation of a neural interface device to enable the local infusion of Ca 2+ antagonists into the visual cortex of adult wild-type and Fmr1 KO mice. While the dura was removed, and infusions were delivered to the cortical surface via an osmotic pump, no significant activation of glial cells was detected. The results indicate that the evaluation of inflammation was sufficiently sensitive in the present study, and that open-dura microfluidic surgery did not cause excessive neural damage nor induce a significant immune response in the week post-surgery. In addition, the spine turnover rate in the neural interface group was similar to that of groups receiving conventional glass windows, or thinned-skull surgery in previous studies 33 . This confirms that neither device implantation nor local infusion affected the rate of spine turnover. After one week, the inlet of the device often clogged, and prevented longer-term analysis, which should be improved in the future studies.
Inhibitors were applied to target a variety of factors, including NMDA receptors, Na + channels, and P-type (Ca v 2.1), N-(Ca v 2.2), R-(Ca v 2.3), and L-type (Ca v 1) Ca 2+ channels in the postsynaptic dendrites. Inhibitor administration was designed to abolish the evoked release of neurotransmitters in the presynaptic terminal. The only remaining sources of Ca 2+ signaling were miniature synaptic transmissions and T-type (Ca v 3) Ca 2+ channels. No increase in cytosolic Ca 2+ concentration was detected in dendrites and spines treated with the inhibitor cocktail. This was monitored via the transfection of the Ca 2+ indicator proteins GCaMP6s, which feature the highest Ca 2+ sensitivity reported to date 34 . We also confirmed that the application of inhibitors abrogated the EE-induced increase in spine turnover in wild-type mice. These results clearly demonstrate that baseline turnover occurs independently of variations in cytosolic Ca 2+ . As baseline spine generation persisted in the complete absence of neuronal Ca 2+ signaling, even when both Na + and Ca 2+ channels were simultaneously blocked, this suggests that the administration of inhibitors successfully blocked activity-dependent plasticity, and that baseline turnover was predominantly governed by Ca 2+ -independent intrinsic structural dynamics. Previous studies have indicated that such intrinsic dynamics might be observed in other cortical regions. Indeed, fractional spine turnover has been detected in the somatosensory and motor cortices in the presence of NMDA receptor inhibitors 6,35 . The present study confirmed that the injection of intraperitoneal MK801 sufficiently blocked NMDA receptors, and reduced the increase in spine generation detected in wild-type mice reared in EE conditions. Because these findings were similar to those obtained using the microfluidic interface device, this suggests that suppression was not caused by the adverse effects of local infusion. Intraperitoneal MK801 infusion could therefore be used to characterize intrinsic spine dynamics without the need for open-dura surgery in future studies.
It is likely that such dynamics are produced by intrinsic fluctuations in spine volume and consequent turnover, as these phenomena are strongly related in vitro 7 . As spine functions are independently modifiable, they therefore behave as physical correlates of memory 36 . However, unlike memories in a manmade computer, dendritic spines are living structures and are inevitably unstable. Fluctuations in spine dynamics might arise due to actin treadmilling 37,38 , dynamic microtubule activity 39 , turnover of postsynaptic-density molecules 40,41 , dynamic maintenance of membrane structures 3,8 , or mitochondrial motility 42 . Instability of the extracellular matrix, which regulates spine stability, might underlie the fluctuations in spine dynamics. However, the mechanisms underlying MMP9 involvement might not significantly contribute to intrinsic fluctuations in wild-type mice. In addition, despite the complete blockade of action potentials, constitutive release of neurotransmitters 43 , hormones 44 , neurotrophins 45 and cytokines might modulate spine dynamics. Spines are surrounded by pulsating blood vessels and glial cells, which are motile and interact dynamically with spines 28,46 . It is remarkable that spine stability can be maintained throughout the lifespan of a mouse under these circumstances 47 . Intrinsic fluctuations in spine dynamics might underlie memory decay 7 , or the maintenance of cortical networks, which remain to be elucidated 7 . Importantly, the intrinsic dynamics of spines observed in the adult neocortex in vivo were activity-independent in the present study, unlike homeostatic synaptic plasticity 48,49 . Moreover, intrinsic dynamics were detected in individual synapses, whereas homeostatic plasticity is a global regulation process of glutamate sensitivity 48,50 .
In particular, the present study provides evidence for abnormal intrinsic spine dynamics in Fmr1 KO mice. These abnormal spine dynamics are likely a result of MMP9 overexpression, as abnormalities associated with the Fmr1 KO phenotype were selectively blocked by MMP9 inhibitors. Such abnormalities might account for the learning disabilities and social impairment reported in individuals diagnosed with ASD 20,51 , as recent studies indicate that behavioral rescue of Fmr1 KO mice was possible following MMP9 KO 21 , and minocycline treatment 14 . The learning deficits associated with ASD might also be related to abnormalities in activity-dependent plasticity [52][53][54][55] . Since MMP9 also impairs activity-dependent plasticity, it is necessary to develop new drugs to selectively prevent the abnormal intrinsic dynamics for clinical application to ASD. Therefore, the present study identified intrinsic spine dynamics in vivo that are fundamental to the understanding of neural function and subsequent disorders. Although the exact mechanisms underlying spine turnover in ASD require further study, the present results provide novel insight with regard to the connection between increased baseline spine turnover and associated learning deficits.

Subjects. A colony of homozygous transgenic mice expressing green fluorescent protein (GFP) under
the control of the Thy1 promoter (Thy1-GFP M-line mice) was generated for the present study 56 . B6.129P2-Fmr1 tm1Cgr /J (Jackson Lab) KO female mice (Fmr1 +/− ) were crossed with males homozygous for Thy-1 GFP to generate GFP-FMR1 −/y mice. Transgenic mice expressing GFP under the control of the Iba1 promoter (Iba1-GFP mice) 57 were used for the in vivo imaging of microglia ( Supplementary Fig. 2a). Under NC, mice were reared in a 20-× 30-× 15-cm transparent plastic cage with shredded paper as bedding. For the EE condition, mice were reared in a larger cage (25 × 41 × 18 cm) with patterned wallpaper and a sawdust bedding (Fig. 2b). Imaging experiments were performed 1 day post-surgery in 2-to 6-month-old mice. After imaging, mice were returned to their respective cages. Intraperitoneal injections of MK801 were performed twice daily (0.25 mg/kg dissolved in saline) 6 , while injections of GM6001 (5-50 mg/kg dissolved in 1-10% DMSO in saline) were performed once daily. Minocycline was dissolved in drinking water (~50 mg/kg/day). All procedures were approved by the Animal Experiment Committee of the University of Tokyo. Procedures were carried out in accordance with the University of Tokyo Animal Care and Use Guidelines. [GC/ml], after dilution with ACSF 10,000 times. These vectors were purchased from U Penn Vector Core (PA, USA). Six-week-old male mice were anesthetized with ketamine (60 mg/kg) and xylazine (10 mg/kg). After shaving the head and applying xylocaine jelly 2% (AstraZeneca, London, UK) to the exposed skin, a round hole with a diameter of 0.5 mm was created using a drill (Coordinates 3 mm posterior and 2.5 mm lateral to bregma). AAV Injection was performed 250 μ m from the dural surface using a glass needle (outer diameter, 40 μ m). The viral solution was injected at a speed of 50 nL/min for a total volume of 100 or 200 nL (mix of virus 1 and virus 2 at a 9:1 ratio) using a syringe pump (Legato 130, KD Scientific, MA, USA). The needle remained in place for 3 min before the needle was removed, and the hole was plugged with cyanoacrylate (Aron-alpha, Toagosei Company, Tokyo, Japan). Ca 2+ imaging was performed 3 weeks after virus injection.
Microfluidic device. The microfluidic interface device was designed as previously described 25 with the following modification. A perfluorocarbon tube with an outer diameter of 200 μ m was connected to the poly (dimethylsiloxane) (PDMS) ring as an inlet (Fig. 1b). No outlet was placed, because the infusion rate (1.0 μ L/h) was relatively slow compared to the generation of cerebrospinal fluid (18 μ L/h) 58 . Beneath the glass cover (150 μ m), a PDMS disk (diameter, 1.2 mm; thickness, ~400 μ m) was inserted to prevent brain pulsation, in addition to a ring-shaped layer of PDMS with a 2.0-mm inner and 2.7-mm outer diameter (Fig. 1b). These layers were bonded together by heating at 235 °C for 150 min.
Cranial open-dura surgery for chronic drug application. Mice were anesthetized with isoflurane (4.5% for induction; 1.0-1.5% for maintenance), delivered via a face mask using an anesthetic regulator (Narcobit-E, Natsume Seisakusho, Tokyo, Japan). The level of anesthesia was assessed by monitoring the tail-pinch reflex. Administration of 20% mannitol (30 μ L/g body weight) was achieved intraperitoneally, while ketoprofen, (2 μ L/g body weight) was administered subcutaneously. Mannitol was applied to shrink the brain and improve separation between the cortex and dura prior to the removal of the dura with a pair of forceps during open-dura surgery. Mice were maintained at 37 °C on a heating pad and the head was stabilized in a stereotaxic frame. Ointment (Tarivid, Santen Pharmaceutical, Osaka, Japan) was applied to the eyes. Once the tail reflex disappeared, the scalp was shaved, washed with ethanol, and removed using a pair of scissors, after which the periosteum was gently removed. A head plate with a 5-mm diameter hole was fixed to the skull using dental cement (Fuji Lute BC, GC Corp., Tokyo, Japan). A small craniotomy (2.7-mm diameter) was performed over the left visual cortex based on stereotaxic coordinates 59 , which were confirmed by intrinsic signal imaging. The craniotomy was achieved using a trephine Scientific RepoRts | 6:26651 | DOI: 10.1038/srep26651 drill (224RF-027, Meisinger, Neuss, Germany) fixed to a stereotaxic instrument. The skull covering the visual cortex was gently removed, and the dura mater was detached from the brain using fine forceps. To avoid bleeding after the surgery, dural vessels in the imaging area were coagulated using heated forceps ( Supplementary Fig. 1a,b). The dura mater underneath the device (Supplementary Fig. 1c) was then partially removed with a pair of forceps.
The device was filled with ACSF (125 mM NaCl, 2.5 mM KCl, 1 mM MgCl 2 , 2 mM CaCl 2 , 1.25 mM NaH 2 PO 4 , 26 mM NaHCO 3 , and 20 mM D-Glucose), and the inlet was plugged with silver wire. The device was positioned within the cranial window and sealed in place with dental cement and dental acrylic (ADFA, Shofu, Kyoto, Japan). The device was protected with a metal cover when the mice were returned to their cages ( Supplementary Fig. 1e). Animals were housed separately after surgery. The infusion solutions were sterilized using a 0.22-μ m syringe filter. Surgical instruments were pre-sterilized using a glass bead sterilizer (Steri 250, Simon Keller, Burgdorf, Switzerland).
Chronic infusion was performed using an osmotic pump (infusion rate, 1 μ L/h; Alzet mini-osmotic pump, model 2001, Alza, CA, USA). Osmotic pumps were implanted subcutaneously onto the backs of mice, and connected to the infusion device at the end of the first imaging session on day 1. Each infusion consisted of one of the following solutions: ACSF, APV, APV+ iVDCC, or APV+ iVDCC+ TTX. In this context, APV refers to amino-5-phosphonopentanoate (1 mM), an NMDA antagonist. iVDCC includes calcicludine (5 μ M) 60 a Ca v 1 channel blocker, ω -conotoxin MVIIC (25 μ M) 61 a selective Ca v 2.1/2 blocker, and SNX-482 (7.5 μ M) 62 a Ca v 2.3 blocker; whilst TTX refers to the Na + channel blocker tetrodotoxin (50 μ M). The final inhibitor concentrations at the surface of the brain were predicted to be 4% of that in the osmotic pump, and should therefore completely block their respective ion channels. For conventional open-skull surgery with a glass window, the dura was kept intact and mannitol was not used during surgery. Circular glass (diameter of 2.7 mm, Matsunami Glass, Osaka, Japan) was fixed with dental cement to the cranial window.
Labeling of microglia and astrocytes. Inflammation was monitored via glial cell imaging. Mice were anesthetized with isoflurane, and perfused with PBS followed by 4% paraformaldehyde (Wako, Osaka, Japan). The brains were removed, fixed overnight in 4% paraformaldehyde at 4 °C, and sliced into 50-or 100-μ m-thick coronal sections using a vibratome (VT1000S, Leica, Nussloch, Germany). Slices were then pre-incubated in blocking buffer (2% horse serum, 0.01% Tween 20, 0.1% NaN 3 in PBS) for 30 min. Microglia and astrocytes were immunolabeled overnight using rabbit anti-Iba-1 antibody (1:1,000 in blocking buffer, cat. #019-19741, Wako) or rabbit anti-GFAP (1:250 in blocking buffer, cat. #ab68428, Abcam, Cambridge, UK), respectively. The samples were washed five times with PBS for 5 min, then incubated with the secondary antibody (1:200 in PBS, Alexa594-labeled goat anti-rabbit IgG, Molecular Probes, OR, USA) for 30 min, then washed another five times with PBS. Slices were mounted onto glass slides and imaged with a two-photon microscope at 830 nm. The numbers of glial cells were calculated between the subpial zone and a region 200 μ m from the pial surface, after which the values were compared with those on the contralateral side. With regard to the brain interface device, either microglial or astrocyte staining was performed after the imaging sessions, and the data were discarded if signs of inflammation were found (> 100 GFAP-positive cells/mm 2 , except for positive controls, Supplementary Fig. 2h). To validate the sensitivity of immunostaining, the brain was intentionally depressed (~200 μ m, ~2 s) using a pair of forceps in several of the mice as a positive control. Two-photon imaging in vivo. Two-photon imaging was performed using upright microscopes (BX61WI, Olympus, Tokyo, Japan; or LSM710NLO, Zeiss, Jena, Germany) equipped with Ti-sapphire lasers (Mai-Tai-DS-HP, SpectraPhysics, CA, USA) set at 950 nm with either a 60× (LUMPlanFI/IR, 0.9 NA, Olympus) or 25× (XLPLN25XWMP2, 1.05 NA, Olympus) water immersion lens. Average excitation power was maintained at < 40 mW under the objective. This power was selected to avoid saturating the fluorescence of dendrites. The fluorescence intensities of dendritic shafts were similar between regions of interests (ROI) and across animals. Mice were anesthetized with a 0.8-1.2% isoflurane-oxygen mixture (Univentor 400 anesthesia unit, Univentor, Zejtun, Malta), and body temperature was maintained at 37 °C using a heating pad. For spine Ca 2+ imaging, anesthesia was maintained with 0.4-0.8% isoflurane. The head was restrained during image acquisition (Supplementary Fig. 1d). Images were obtained from layer V pyramidal cells with apical dendrites in layer I. Dendrites on which spines were well separated from another were selected for analysis. Infusions were administered immediately after the first imaging session on day 1 (Fig. 2a).
Image processing and analysis were performed using ImageJ. For spine Ca 2+ imaging, images were acquired at 10-15 frames/s and the backgrounds were subtracted. Oval ROIs were placed over each spine, while polygonal ROIs were placed over the dendritic shaft (Fig. 1c). Δ F/F 0 was calculated for each ROI from each frame as (F − F 0 )/F 0 , where F 0 was the mode of fluorescence signal during the 4-min imaging session. Calcium signals that crossed three standard deviations for more than two consecutive frames were defined as dendritic shaft responses. Active dendritic shafts were defined as those producing one or more responses during the 4-min imaging session. Dendritic Ca 2+ transients invaded the spines in most instances (Fig. 1d-j). To identify spine-specific calcium responses, the dendritic shaft traces were scaled as much as possible to fit with the spine signals. If calcium transients in the spines exceeded dendritic transients on more than three occasions, such spines were classified as active spines. All spines on the selected dendrites were analyzed. Such measurements were performed on one to four dendrites per cell. If any active spines were identified within these images, the dendrite was categorized as "dendritic + synaptic Ca 2+ transients" (Fig. 1f). If no active spines were identified, but the dendrite was active, the dendrite was categorized as "dendritic Ca 2+ transients" (Fig. 1g-j). If neither active spines nor shafts were identified, the dendrite was categorized as "no Ca 2+ transients" (Fig. 1k,l).
For dendrite structural imaging, three-dimensional reconstructions of dendritic morphology were generated from a stack of 19-71 two-dimensional images, each separated by 0.5 μ m. Spine turnover was assessed on every 2-day interval post-surgery (Fig. 2a). Spines were identified as protrusions from dendrites with an apparent head Scientific RepoRts | 6:26651 | DOI: 10.1038/srep26651 structure (head diameter/neck diameter > 1.2 and head fluorescence/neck fluorescence > 1.2). Spines emanating from the dendrite perpendicular to the imaging plane were counted only when the head was clearly visible in a section. Filopodial protrusions without a head structure were excluded from analysis. Spines were considered the same between sessions if their positions remained the same distance from adjacent landmarks. The rates of dendritic spine generation and elimination were defined as the percentage of spines that appeared and disappeared, respectively, between two successive imaging sessions with an interval of 2 days. Dendritic shaft lengths were analyzed using ImageJ Simple Neurite Tracer plugin. The average length of the analyzed dendritic shafts was 488 ± 60 μ m (N cell = 126). Data were discarded if any signs of damage were detected, included bleeding, a dim window, swelling/blebbing, or a feeble fluorescence level compared to the previous imaging session. Spine turnover rates at 2-4 months and 4-6 months old did not significantly differ, except for a slight reduction in elimination rate with regard to APV infused mice raised in NC, and a reduction in generation for KO mice raised in EE conditions ( Supplementary Fig. 4a-h).
Statistical analyses. Spine generation and elimination rates were assessed at 2-day intervals, and averaged within each group, except for Supplementary Fig. 3, wherein spine generation and elimination rates were compared between the initial 2-day imaging interval and subsequent intervals. All data are presented as mean ± SEM, and were analyzed using a Fisher's exact test (Fig. 1f), Kruskal-Wallis test (Figs 2-4), or underwent further analysis using a Steel's test. A Mann-Whitney test was used to analyze the data presented in Supplementary Figs 2 -4. No statistical methods were used to predetermine sample sizes, but the sample sizes used were similar to those reported in previous publications. Randomization and blinding were not used in this study.