Figure 4: Correlative imaging of sample scrap from preserved and altered parchment “1639”. | Scientific Reports

Figure 4: Correlative imaging of sample scrap from preserved and altered parchment “1639”.

From: Correlative nonlinear optical microscopy and infrared nanoscopy reveals collagen degradation in altered parchments

Figure 4

Correlative imaging and IR nanoscopy characterization from (A–E) preserved and (G–K) altered parchment: (A,G) optical microscopy, (B,H) NLO microscopy, (C,I) AFM topography, nanoIR mapping at (D,J) 1724 cm−1 and (E,K) 1660 cm−1. (F,L) NanoIR spectra recorded between 1600 and 1800 cm−1 from local areas (colored points in AFM images) in the preserved and altered samples. The carbonyl vibration at 1724 cm−1 clearly appears in the nanoIR spectra from the altered parchments and (J) nanoIR mapping at this wavenumber exhibits strong signals on the contrary to (D) the mapping from the preserved one.

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