Figure 1: Immersed super-fine electrospray-based miniaturized in vitro compartmentalization. | Scientific Reports

Figure 1: Immersed super-fine electrospray-based miniaturized in vitro compartmentalization.

From: A bulk sub-femtoliter in vitro compartmentalization system using super-fine electrosprays

Figure 1

A schematic illustration of the experimental setup is shown. An inkjet nozzle head (filled with a nanomolar solution of template DNA and a cell-free gene expression system) immersed in an immiscible oil phase can ultra-rapidly produce monodisperse water-in-oil femtoliter-scale droplets in bulk on a mega-scale. The enlarged diagram shows the encapsulation of the in vitro protein expression system in a water-in-oil droplet or in an agarose-in-oil gel bead.

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