Table 1 Summary of anesthesia and treatment protocols.

From: A novel anesthesia regime enables neurofunctional studies and imaging genetics across mouse strains

Anesthetic agent (application route) Respiratory conditions Animals Initial (priming) dose Maintenance dose Challenge
Isoflurane (Inhalation) Spontaneously breathing (carrier gas composed of oxygen and air, 1:5 v/v) rat Sprague Dawley 4% 2.0–2.3% Acetazolamide (30 mg/kg)
mouse C57BL/6J 2–3% 1.8–2.0% Acetazolamide (30 mg/kg) Olanzapine (3 mg/kg; C57BL/6J mice)
BTBR T+tf/J
CD1
Medetomidine a (s.c.) rat Sprague Dawley 0.2 mg/kg (+4% isoflurane) 0.1 mg/kg/h Acetazolamide (30 mg/kg)
mouse C57BL/6J 0.3 mg/kg (+2.5–3% isoflurane) 0.6 mg/kg/h Acetazolamide (30 mg/kg) Olanzapine (3 mg/kg)
Etomidate b (i.v.) rat Sprague Dawley 5 mg/kg/min (3 min) (+4% isoflurane) 0.25 mg/kg/min Acetazolamide (30 mg/kg)
mouse C57BL/6J 4 mg/kg/min (3 min) (+2.5–3% isoflurane) 0.75 mg/kg/min Acetazolamide (30 mg/kg) Olanzapine (3 mg/kg; C57BL/6J mice) Electrical hindpaw stimulation (2 mA; ventilated C57BL/6J mice)
BTBR T+tf/J 1.0 mg/kg/min
CD1 1.5 mg/kg/min
  1. ai.v., intravenous.
  2. bs.c., subcutaneous.