Figure 5: Tyramide signal amplification (TSA) assays with HRP-GST-ABD or HRP-conjugated secondary antibodies in the presence of primary antibodies derived from rabbits. | Scientific Reports

Figure 5: Tyramide signal amplification (TSA) assays with HRP-GST-ABD or HRP-conjugated secondary antibodies in the presence of primary antibodies derived from rabbits.

From: A Recombinant Secondary Antibody Mimic as a Target-specific Signal Amplifier and an Antibody Immobilizer in Immunoassays

Figure 5

SKBR3 and KB cells overexpress HER2 and integrin αβγ3 receptors, respectively, on their surfaces. SKBR3 (A,B) and KB (C,D) cells were treated with anti-HER2 rabbit (A,B) and anti-integrin αβγ3 mouse (C,D) primary IgGs, respectively, and subsequently incubated with HRP-conjugated anti-rabbit secondary antibodies (A,D) or HRP-GST-ABD (B,C) in the presence of TSA reagents. Treatment amounts for primary anti-HER2 rabbit and anti-integrin αβγ3 mouse antibodies are indicated at the top of each column. 1AB 1x represents the typical amount of primary antibodies used for TSA assays.

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