Figure 4: GST-ABD serves as an anchoring adaptor on the surface of GSH-coated plates for immobilizing antigen-capturing anti-bodies in an orientation-controlled manner in sandwich-type indirect ELISA. | Scientific Reports

Figure 4: GST-ABD serves as an anchoring adaptor on the surface of GSH-coated plates for immobilizing antigen-capturing anti-bodies in an orientation-controlled manner in sandwich-type indirect ELISA.

From: A Recombinant Secondary Antibody Mimic as a Target-specific Signal Amplifier and an Antibody Immobilizer in Immunoassays

Figure 4

(A) Scheme showing the adaptation of GST-ABD as an anchoring adaptor for antigen-capturing antibodies. (B,C) GST-ABDs are spread on the surface of GSH-coated plates and saturated with capturing antibodies, either anti-BSA rabbit IgGs (B) or anti-EpCAM rabbit IgGs (C). Various amounts of BSA (B) or EpCAM (C) are added. Linear responses for each measurement are plotted as insets of each graph.

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