Figure 3: HRP-GST-ABD mimics signal-amplifying secondary antibodies in indirect ELISA. | Scientific Reports

Figure 3: HRP-GST-ABD mimics signal-amplifying secondary antibodies in indirect ELISA.

From: A Recombinant Secondary Antibody Mimic as a Target-specific Signal Amplifier and an Antibody Immobilizer in Immunoassays

Figure 3

(A) Scheme showing different combinations of primary antibodies with either HRP-conjugated secondary antibodies or HRP-GST-ABD. (B–E) BSA (B,C) or EpCAM (D,E) are immobilized on the surface of the ELISA plates and various concentrations of either rabbit anti-BSA (B) or mouse anti-BSA primary antibodies (C) or either rabbit anti-EpCAM (D) or mouse anti-EpCAM primary antibodies (E) are applied. Linear responses of each measurement are plotted as insets of each graph.

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