Figure 4: Time lapse acquisition of live dechorionated zebrafish embryo with two-photon excitation iSPIM. | Scientific Reports

Figure 4: Time lapse acquisition of live dechorionated zebrafish embryo with two-photon excitation iSPIM.

From: 4D (x-y-z-t) imaging of thick biological samples by means of Two-Photon inverted Selective Plane Illumination Microscopy (2PE-iSPIM)

Figure 4

Panel (a) shows fluorescence temporal evolution of a 18 hpf embryo after staining with the vital dye 4-Di-2-Asp (DiAsp). Panel (b) shows temporal evolution of a 22 hpf embryo after staining with Hoechst 33342 (b). Scale bar 50 μm. Excitation wavelength λ = 720 nm for DiAsp (I = 20 kW/cm2), and 740 nm for Hoechst (I = 33 kW/cm2). Illumination and detection objective Nikon CFI Plan Fluor 10X W, NA 0.3. Exposure time 100 ms/frame (0.04 VpS).

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