Table 1 Functional and structural avidities of the A2/MART1 TCRs.

From: CD4+ and CD8+ TCRβ repertoires possess different potentials to generate extraordinarily high-avidity T cells

Clone T cells derived from TRBV CDR3β TRBJ Structural avidity* without CD8 Structural avidity with CD8 EC50(μg/ml) EC50(μg/ml)
Functional avidity without CD8 Functional avidity with CD8
Cl.7E CD4 27 CASSRDFGNTIYF 1–3 0.010 0.006 0.102 0.071
Cl.3P CD4 5–1 CASSLTGGYGYTF 1–2 0.013 0.012 0.116 0.058
Cl.830 CD8 27 CASSLGGAYEQYF 2–7 0.013 0.008 0.056 0.036
Cl.8H CD4 27 CASSPLGAMEQYF 2–7 0.014 0.008 0.063 0.040
Cl.1606 CD8 27 CASSLLGSYEQYF 2–7 0.015 0.008 0.031 0.031
Cl.7Q CD4 27 CASSPYMMNTEAFF 1–1 0.016 0.009 0.065 0.043
Cl.1593 CD8 27 CASGNNQPQHF 1–5 0.016 0.008 0.104 0.047
Cl.9J CD4 5–1 CASSWTGDGYTF 1–2 0.018 0.010 0.075 0.071
Cl.2J CD4 27 CAASMGQGFGEQFF 2–1 0.018 0.008 0.100 0.072
Cl.2E CD4 27 CASSWDWGNIQYF 2–4 0.019 0.014 0.070 0.056
Cl.11E CD4 2 CASSVMAPLHF 1–6 0.022 0.011 0.121 0.074
DMF5‡ CD8 6–4 CASSLSFGTEAFF 1–1 0.022 0.013 0.363 0.102
Cl.4D CD4 5–1 CASSWAGTGSEQYF 2–7 0.030 0.022 0.179 0.073
Cl.6X CD4 2 CATGVTDTQYF 2–3 0.039 0.018 0.150 0.083
Cl.10V CD4 5–1 CASSLQGANGELFF 2–2 0.043 0.033 0.405 0.140
Cl.6B CD4 2 CASSEVAWQFF 2–1 0.044 0.013 0.600 0.120
Cl.1086 CD8 27 CASSLHGPGGYTF 1–2 0.048 0.013 0.554 0.076
Cl.3J CD4 5–1 CASSLMGTEAFF 1–1 0.050 0.025 1.451 0.263
Cl.1599 CD8 27 CASSFLGAMAEAFF 1–1 0.069 0.018 0.053 0.032
Cl.6I CD4 2 CATGRGATQYF 2–3 0.070 0.020 0.665 0.125
Cl.788 CD8 27 CASGPSYEQYF 2–7 0.071 0.013 0.880 0.121
Cl.794 CD8 27 CASSLLGDYGYTF 1–2 0.082 0.018 0.067 0.043
Cl.11C CD4 2 CASDEGFGYTF 1–2 0.108 0.018 2.460 0.292
Cl.1574 CD8 27 CASSPWERINTEAFF 1–1 0.110 0.021 0.076 0.052
Cl.758 CD8 27 CASSPRLAGDGELFF 2–2 1.610 0.041 0.342 0.096
Cl.523 CD8 27 CASGSYEQYF 2–7 n.m 0.064 n.m 0.748
Cl.413 CD8 27 CASSVFGGDMGEKLFF 1–4 n.m 0.303 n.m 2.337
  1. *Structural avidity, expressed as EC50 in μg/ml, was defined as the concentration of A2/MART1 multimer required to achieve 50% of maximal multimer staining.
  2. Functional avidity, expressed as EC50 in μg/ml, was defined as the concentration of peptide required to achieve 50% of maximal response.
  3. DMF5 is a high affinity A2/MART1 TCR reported by Johnson et al.28 n.m, not measurable.