Figure 3 | Scientific Reports

Figure 3

From: Effects of hydrogen-rich water on depressive-like behavior in mice

Figure 3

Caspase-1 activity in hippocampus and prefrontal cortex.

ImmunoChemistry Technologies’ FAM-FLICA™ Caspase-1 Assay Kit was used to detect active caspase-1. The caspase-1 activity was expressed as relative of the control group. (e) Caspase-1 activity in hippocampi of the stress group mice increased when compared to the control, hydrogen-rich water and hydrogen-rich water + stress groups (F1, 16 = 4.814, P = 0.0433, n = 5, 5, 5, 5), which indicated activation of inflammasome. (j) Caspase-1 activity in prefrontal cortexes of the stress group mice increased significantly when compared to the control group (F1, 16 = 44.50, P < 0.0001, post hoc ***P < 0.0001, n = 5, 5, 5, 5) and compared to the hydrogen-rich water (F1, 16 = 30.06, P < 0.0001, post hoc ***P < 0.0001, n = 5, 5, 5, 5). Chronic unpredictable mild stress-induced increasing of caspase-1 activity was impeded by the hydrogen-rich water as shown in the comparison between hydrogen-rich water + stress group and stress group (F1, 16 = 30.06, P < 0.0001, post hoc ***P < 0.0001, n = 5, 5, 5, 5). Representative FLICA photographs from hippocampus and prefrontal cortex were shown in (a–d) and (f–i) respectively. No significant differences observed among the control, hydrogen-rich water and hydrogen-rich water + stress groups. Data was analyzed using a two-way ANOVA followed by the Bonferroni’s multiple comparisons post hoc test. Error bars represent s.e.m.

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