Figure 5 : Effect of the membrane permeabilization of the parasite-infected red blood cells on the cytosolic Ca2+ level in P. falciparum.
From: Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor
Schematic indicates the status of the existence of Ca2+ in the different compartments in the parasite-infected red blood cell (iRBC) without treatment (a), treated with streptolysin O (SLO) (b), or treated with saponin (c). Time courses of the cytosolic Ca2+ level with the addition of 15 μM cyclopiazonic acid (CPA) and 10 μM A23187 to non-treated iRBCs in Ca2+-containing RPMI medium (a), with the addition of 3 μM CPA and 2 μM A23187 to SLO-treated iRBCs in Ca2+ free medium (b), or with the addition of 3 μM CPA and 2 μM A23187 to saponin-treated iRBCs in Ca2+ free medium (c). A lower concentration of CPA and A23187 was used to avoid the damage to the SLO-treated and saponin-treated iRBCs. The traces are generated from the mean and standard error of mean of 4 independent experiments.
