Figure 2 : Cytosolic Ca2+ concentration in the different developmental stages of P. falciparum.
From: Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor
(a) FRET signals from amoeboid ring (n = 11), trophozoite (n = 18), schizont (n = 10), merozoite (n = 10), and gametocyte (stage III (n = 6) and stage IV-V (n = 7)) stages of the parasite. Bright field (BF), merged image of BF, merged image (BF-CFP-YFP), FRET (YFP/CFP) signals, and calculated Ca2+ concentration with pseudo color are shown. Purple to red color scale in FRET (YFP/CFP) signals and calculated Ca2+ concentration represent low to high FRET efficiency (0 to 2.5) and 0 to 1000 nM Ca2+, respectively. Scale bar, 4 μm. (b) Calculated cytosolic Ca2+ concentrations of parasites with median and interquartile range are shown for each stage. **p = 0.0012, ***p < 0.0001 by Mann-Whitney U-test.
