DNA damage in mouse T cells.
(a) A drop of blood from mouse tail was collected in sodium-heparinized micro-hematocrit capillary tubes and irradiated ex vivo with 2 Gy. The tubes were incubated at 37 °C and blood smears were performed 1 and 4 h later. After air drying, they were stained for the CD3 T cell receptor and γH2AX. Imaging was performed using LSM. Representative pictures are shown (Z-stacks). The nucleus is stained blue; the CD3 T cell receptor red and γH2AX green. Asterisk indicates significant difference to the control and 4 h measure points (p<0.05). (b) C57 BL/6 mice were treated i.p. with 200 mg/kg TMZ or DMSO (solvent control) and blood was collected 16 h later for blood drop method. There is a significant increase of DNA damage (p < 0.05) in CD3+ T cells if mice were treated with temozolomide compared to the DMSO control. In the pictures on the top, the nucleus is stained blue, CD3 T cell receptor red and γH2AX green.