Figure 1 | Scientific Reports

Figure 1

From: The γH2AX DNA damage assay from a drop of blood

Figure 1

Blood for γH2AX staining.

(a) PBMCs obtained by Ficoll gradient centrifugation. 5–10 ml peripheral blood were usually collected in heparinized tubes, which is layered onto a Ficoll gradient. Following centrifugation, the layer containing PBMCs was harvested, washed four times, concentrated by centrifugation and fixed onto glass slides. (b) A drop of blood is taken up in a heparinized glass micro-hematocrit capillary tube and spread onto one or several glass slides.

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