Figure 3 : More LPDCs express TLR5 than spleen DCs and most CD172α+ LPDCs are also TLR5+.

From: TLR5 mediates CD172α+ intestinal lamina propria dendritic cell induction of Th17 cells

Figure 3

Spleen cells and LP cells were isolated from C57BL/6 mice. After depleting CD4+ T cells, CD4 cells were stained with CD11c, CD11b, CD103, and TLR5 and analyzed by flow cytometry. (A) CD11c+ cell expression of TLR5 was shown. (B) TLR5 expression by CD11b+ and CD11b DCs by gating on CD11c+ population. (C) CD11c+ DC expression of CD103 and CD11b by gating on CD11c+ population. (D) Expression of TLR5 and CD172α by CD103+ DC and CD103 DCs in LP of wild-type C57BL/6 mice by gating on CD11c+CD11b+ population. (E,F) CD172α expression by CD103+ DC and CD103 DCs in LP of wild-type (E) and TLR5 KO C57BL/6 mice (F) by gating on CD11c+ population. FACS plots are reflective of 2–4 independent experiments.