Antibody epitopes of selected antigens identified by peptide array technology.
Binding of human IgG to 15-mer peptides overlapping with 14 amino acids on a peptide array spanning the entire length of each of the selected antigens. IgG binding was measured with a Cy3-conjugated goat anti-human antibody after incubation with plasma pooled from six adult donors. The black bars indicate fluorescence intensity for each of the peptides in arbitrary units that arise from summarizing over the R, G and B channels in the resulting image file. Peptides were randomly distributed on the array and data was mapped back in the right sequence order of the antigen after the experiment.