IgG responder analysis of selected antigens.
Total IgG specific for selected antigens was measured by ELISA in plasma from (A) adults (n = 32) and (B) school aged children from 5–15 years (n = 30). Lines represent medians of the EC50 calculated by a sigmoidal fitting of data points from a 10-fold plasma dilution series. Responses too low for accurate fitting were adjusted to EC50 = 1. Responses for spy1801 have been reported for this donor panel in another publication21, but plasma samples have been re-run for this antigen along with the other antigens in this study for direct comparison.