Table 1 Kinetic rate constants (determined by SPR) for the interaction of apo BtuF with fresh BtuC2D2 or BtuC2D2 that had worked for 7 hours.

From: The uncoupled ATPase activity of the ABC transporter BtuC2D2 leads to a hysteretic conformational change, conformational memory and improved activity

  Fresh Worked T. test
kon (105 M−1, s−1) 2.8 ± 0.7 2.9  ± 0.6 ns
koff (10−5 s−1) 1.2 ± 0.3 1.1 ± 0.4 ns
Kd (10−11 M) 3.6 ± 0.8 3.7 ± 0.5 ns
Rmax (Ru) 67.7 ± 1.8 93.3 ± 1.4 9.20E-06
  1. Rmax describes the maximal number of binding events and is given in response units (Ru). Errors represent standard deviations of five independent experiments.