Light intensity affects the uptake and metabolism of glycine by pakchoi (Brassica chinensis L.)

The uptake of glycine by pakchoi (Brassica chinensis L.), when supplied as single N-source or in a mixture of glycine and inorganic N, was studied at different light intensities under sterile conditions. At the optimal intensity (414 μmol m−2 s−1) for plant growth, glycine, nitrate, and ammonium contributed 29.4%, 39.5%, and 31.1% shoot N, respectively, and light intensity altered the preferential absorption of N sources. The lower 15N-nitrate in root but higher in shoot and the higher 15N-glycine in root but lower in shoot suggested that most 15N-nitrate uptake by root transported to shoot rapidly, with the shoot being important for nitrate assimilation, and the N contribution of glycine was limited by post-uptake metabolism. The amount of glycine that was taken up by the plant was likely limited by root uptake at low light intensities and by the metabolism of ammonium produced by glycine at high light intensities. These results indicate that pakchoi has the ability to uptake a large quantity of glycine, but that uptake is strongly regulated by light intensity, with metabolism in the root inhibiting its N contribution.

nitrate, and amino acids, show that uptake of one form of nitrogen has an effect on the uptake of other forms 17,22 . Ammonium hindered the uptake of nitrate, and the external addition of amino acids inhibited the uptake of both ammonium and nitrate 23 . Considering differences between N sources in the energy demands of assimilation and the interactions between N forms, light intensity has a great effect on the uptake of N 24 .
Light intensity varies considerably, regardless of the latitude, day or night, altitude, or ecological niche of plant species. However, it is unclear which forms of N plants acquire under different light intensities, especially organic forms of N. Xingliang studied the effects of light on short-term competition for nitrate, ammonium, and glycine between maize and rhizosphere microorganisms 11 ; it was demonstrated that high levels of light intensity significantly increased maize uptake of 15 NO 3 − and glycine 14 C, but did not significantly affect the uptake of 15 NH 4 + or 15 N-glycine. This suggests that light intensity may alter the competitive relationships between rhizosphere microorganisms and maize roots. However, there is a lack of detailed research exploring the effect of light intensity on the uptake of amino acids and the influence mechanism of it.
Pakchoi (Brassica chinensis L.) is one of the most important vegetables in China, planted over a large north-south range 25 . We used pakchoi as our test material, and cultivated it in a sterile environment. The objectives of this study were to (1) determine whether amino acids can play an important role in N nutrition for pakchoi in a sterile environment, (2) research the effect of light intensity on the relative uptake of organic and inorganic N by pakchoi, and (3) reveal the mechanism by which pakchoi alters its uptake of glycine under different light intensities.

Results
Pakchoi biomass and long-term N uptake under single or mixed N sources. Under the mixed N sources, light intensity had a significant effect on N uptake by pakchoi and plant growth ( Fig. 1 p < 0.05). The fresh weight of the shoot and root increased with light intensity up to 414 μ mol m −2 s −1 . Specifically, the fresh weight of plants grown under 540 μ mol m −2 s −1 light was significantly lower than that of plants grown under 414 μ mol m −2 s −1 light (Fig. 1A). N uptake followed a similar trend to that of pakchoi growth. A curve fitted to light intensity and total N uptake data (mg plant −1 ) yielded the following equation: y = − 2E − 05x 2 + 0.0151x + 0.5961 (R 2 = 0.95 ** ), showing that the optimal light intensity for pakchoi N uptake was approximately 360 μ mol m −2 s −1 (Fig. 1B). Under the single N source of 3 mM glycine, pakchoi growth and glycine-15 N uptake were significantly affected by light intensity (Fig. 2 p < 0.01). The biomass and 15 N uptake of the shoot and root under 360 μ mol m −2 s −1 light were significantly higher than 90 and 540 μ mol m −2 s −1 light, with similar results being obtained for the combined N sources (Fig. 2B).
Photosynthetic characteristics of pakchoi. Light had a strong effect on the photosynthetic characteristics of pakchoi leaves ( Table 1). The photosynthetic rate, conduction to H 2 O, and transpiration rate all increased with light intensity, and peaked at 414 μ mol m −2 s −1 ; these values were slightly lower in plants grown under 540 μ mol m −2 s −1 light compared to plants grown under 414 μ mol m −2 s −1 light. Intercellular CO 2 concentration showed an opposite trend, decreasing with increasing light intensity. The lowest intercellular CO 2 concentration was found in plants grown under 414 μ mol m −2 s −1 light.  Uptake of glycine, nitrate, and ammonium. Light intensity had a strong effect on the uptake of different N forms (Fig. 3). Shoot 15 N from glycine and nitrate increased with increasing light intensity (up to 414 μ mol m −2 s −1 ), while ammonium-15 N in the shoots under 414 μ mol m −2 s −1 light was similar to those under 288 and 540 μ mol m −2 s −1 light (Fig. 3A). In the shoots, nitrate-15 N uptake was significantly higher than glycine-15 N uptake by pakchoi, accounting for 31-45% total N (Fig. 3B). In comparison, nitrate-15 N was significantly lower than glycine-15 N in the roots, with the former only accounting for 17-23% and the latter 40-45% total N (Fig. 3D). Furthermore, the N contribution of glycine, nitrate, and ammonium for pakchoi shoots under 36 μ mol m −2 s −1 light were 18%, 45%, and 36%, respectively. In comparison, these values were 29%, 39%, and 31% under 414 μ mol m −2 s −1 light, respectively (Fig. 3B). Glycine was an important source of N for pakchoi, accounting for 18-29% total shoot N uptake, and peaking at 414 μ molm −2 s −1 light (Fig. 3B).
Short-term uptake and transformation of glycine. Light intensity affected the uptake and root to shoot transportation of glycine-N (Fig. 4). Total uptake and active uptake of glycine-15 N under optimum light intensity (360 μ mol m −2 s −1 , for N uptake) were greater than the uptake of these parameters documented under lower light intensity. In comparison, there was no significant difference in uptake between optimum and over-high light intensity (540 μ mol m −2 s −1 ). Although active uptake under the over-high light intensity was slightly lower than that documented at the optimum light intensity, the uptake and transportation from root to shoot of passive uptake glycine-15 N were significantly higher than that under the optimum light intensity (Fig. 4C). The passive uptake of glycine-15 N increased with light intensity (Fig. 4A,B).
Activity of glycine metabolic enzymes. GPT enzyme activity in the shoots and GS activity in the shoots and roots varied with light intensity ( Table 2). In contrast, the activities of GPT in roots, and GOT in shoots and roots showed no significant difference among the different light-intensity treatments. GS activity in the shoots and roots under optimum light intensity was higher compared to over-high light intensity. Amino acids contents of pakchoi. The amino acids and NH 3 in the shoots and roots differed under optimum light (360 μ mol m −2 s −1 ) and over-high light (540 μ mol m −2 s −1 ) over a 12 h period (Fig. 5). In the shoots, the contents of aspartic acid, proline, and valine under over-high light were significantly higher than optimum light. In contrast, serine, glycine, leucine, lysine, and arginine contents were lower under over-high light compared to optimum light (Fig. 5A). Under the over-high light, the roots contained more proline, cysteine, and methionine, with lower glycine and tyrosine than under optimum light (Fig. 5B). Seedlings that grew under over-high light contained more NH 3 in the shoots and roots, with the sum of the amino acids and NH 3 in the shoots being significantly higher than that under optimum light conditions (Fig. 5A,B). Discussion Effect of light intensity on pakchoi growth. Light energy is used to produce NADPH and ATP in the light reactions of photosynthesis and is important for plant growth 26 . The biomass of pakchoi roots and shoots increased with increasing light intensity up to 414 μ mol m −2 s −1 . Under low light intensity, carbohydrate biosynthesis and carbon fixation are limited by ATP availability 27 , whereas excessive light intensity generates oxygen radicals and causes photoinhibition 28 . Both of these conditions seriously limit primary productivity. The highest light intensity used in our experiments was unlikely to cause severe stress, as the intensity of sunlight may reach around 2000 μ mol m −2 s −1 in the middle of the day 29 . However, when exposed to a constant intensity of light for 12 h each day, the optimum light intensity for pakchoi seedling growth was approximately 400 μ mol m −2 s −1 , with 540 μ mol m −2 s −1 light having a negative effect on growth over 25 days of culture. The light saturation point differs among plant species, as shown by Carrigan, who reported that 16-h daily exposure to 1000 μ mol m −2 s −1 light has a passive impact on tomato growth, altering leaf morphology and stomatal behaviour, as well as reducing plant height and photosynthesis rates 13 .
Effect of light intensity on N uptake. Light intensity had a strong effect on the uptake of N and different N forms. Under controlled growth conditions with constant light intensity throughout the day, 360 μ mol m −2 s −1 was the optimum light intensity for N uptake. Ammonium is not the preferred N source for most plant species; however, the N contribution of ammonium was higher in the shoots than those of nitrate and glycine under 288 and 540 μ mol m −2 s −1 light. This result indicates that light intensity changed preferred N uptake by pakchoi. Most plants tend to take up nitrate, even though ammonium may require less processing than nitrate. Nitrate taken up

Table 2. The effect of light intensity on the activity of glycine metabolic enzymes in Brassica chinensis L.
Values represent the mean ± SE (n = 4). Different letters in each column indicate significant differences between treatments at the p < 0.05 level. Abbreviations: GPT, glutamic-pyruvic transaminase enzyme; GOT, glutamic oxalacetic transaminase; GS, glutamine synthetase. by roots must be converted to ammonium by nitrate reductase, and additional metabolic processes are required for the synthesis of amino acids 30 . However, nitrate taken up by roots was mostly transported to shoots, whereas ammonium was assimilated to amino acids in the roots 31 . The difference in the metabolism process had a great effect on N uptake. Optimum light intensity provided enough energy, in addition to improving the passive uptake and transportation of N, which increased the uptake of nitrate. The uptake of different nitrogen was regulated by many factors, with the single factor analysis showing that light intensity is important. Pakchoi absorbs nitrate to a greater degree than ammonium or organic N 32 . In our study, the quantity of 15 N-nitrate in pakchoi shoots exceeded that of other N forms under optimum light intensity; however, the 15 N-nitrate in the pakchoi roots was much lower than other N forms. In addition, the N contribution of nitrate in the shoots had a significant negative correlation with its N contribution in the roots. Research clearly showed that nitrate taken up by the roots is mostly transported to the shoots, whereas ammonium is assimilated to amino acids in the roots 31 . Under different light intensities, we documented 1) lower 15 N-nitrate in the roots, but higher uptake in shoots, 2) higher 15 N-glycine content in the roots, but lower content in the shoots, and 3) greater change in the contribution of each N form in the shoots compared to the roots. These results indicate that, first, most 15 N-nitrate uptake by the root was rapidly transported to the shoot, with the pakchoi shoot having a powerful assimilative capacity. Second, N contribution of glycine was not limited by uptake, but was limited by assimilation ability or the transportation of amino acids and its production in the roots. Third, light intensity affected the N contribution of different N forms by regulating the metabolism of leaves, rather than root uptake. N contribution of glycine. Plants have the ability to take up and metabolize a large number of amino acids.
In the present study, glycine accounted for 18%-29% total N uptake over 25 days of cultivation. As the amino acids transporters that have been identified in most crops 33,34 , the uptake rates of organic N were similar to those of inorganic N 35 ; thus, organic N should not be overlooked as an important source of N for crops. However, the test was completed in a sterilized environment, bypassing the competition of microorganism, which is considered to be more competitive for organic N. Furthermore, low concentrations of amino acids in soil solutions limit the N-contribution of amino acids . However, soil amino acids have fast turnover rates (i.e., a few hours); hence, the flux of amino acids into plants maybe large, despite low concentrations in soil solution 17 . Whether amino acids have an unrecognized or negligible role in plant nutrition could not be determined, because accurate method to determine the quantitative contribution of organic nitrogen is still lacking 36 . However, we showed that pakchoi possesses the ability to uptake and metabolism a large quantity of glycine, which warrants further investigation.
There is evidence of direct organic N uptake by plants in various plant community types 34 . Many of these ecosystems occur in low-temperature zones, such as the arctic 37 , alpine tundra 22 , boreal forest 38 , and heath lands 1,39 , where there is a lack of inorganic N and relatively slow mineralization of organic N. Mean light intensity differs markedly with latitude, and is higher in temperate, subtropical, and tropical zones than boreal areas. In the present study, glycine contributed a substantial proportion of total N (up to 29%) in pakchoi, which was higher under high light intensity than under low light intensity. However, in the natural environment, high temperature occurs in parallel with high light intensity in most cases, which promote strong organic N mineralization. Roots were regarded as stronger competitors for inorganic N than microorganisms, whereas microorganisms were strong competitors for organic N 11 . So, increasing light intensity caused the plant uptake of amino acids to increase, but a decline in microorganism activity. The effect of light intensity and the interaction effect of light and temperature on the uptake of organic N in natural environments require more detailed research.
Potential mechanisms for the effect of light intensity on glycine uptake. The N contribution of glycine was highest under the optimum light intensity in both mixed nitrogen sources and a single source of glycine. This result may be due to energy support from photosynthesis, increased glycine uptake and transport, or increased assimilation of glycine N.
In the present study, light intensity had a strong effect on the photosynthetic characteristics of plants. For plants cultivated at 540 μ mol m −2 s −1 light, the photosynthesis rate was lower than that of plants cultivated at 414 μ mol m −2 s −1 light; however, the intracellular CO 2 concentration was relatively low in both plant groups. We speculated that the low internal CO 2 concentration in plants grown at 414 μ mol m −2 s −1 light was caused by the consumption of CO 2 during photosynthesis. In contrast, for plants grown under 540 μ mol m −2 s −1 light, the observed low internal CO 2 concentration was caused by the closure of leaf stomata. Thus, photosynthesis was limited by high light intensity. This phenomenon may have inhibited the active uptake of amino acids (Fig. 4A), as this process requires energy.
The majority of amino acids absorbed by plants are taken up through a series of co-transporters that are driven by H + -ATPase 40 . This result is consistent with our observation that glycine absorption in CCCP un-treated plants was 3.3-to 4.1-fold greater than that of CCCP-treated plants, because CCCP inhibits active uptake of glycine. Although active uptake represented approximately 70% of total absorbed glycine, passive uptake also contributed to glycine uptake, especially at optimum and high light intensities. The active and passive uptake of glycine in plants exposed to optimum light intensity was significantly higher than those in plants exposed to low light intensity. This result indicates that uptake was likely the rate-limiting step for glycine utilization in low intensity light. Over a 10-d culture period, glycine uptake was significantly higher under optimum light intensity than under over-high light intensity. However, in the short-term uptake test, the active uptake of glycine was similar in plants exposed to optimum and high light intensity, whereas the passive uptake of glycine was greater in plants exposed to over-high light intensity than in those exposed to optimum light intensity, indicating that uptake was not the limiting step under the over-high light intensity. N contribution is regulated by uptake, transportation, and assimilation. The long-term uptake test shown that glycine uptake in over-high light was significantly lower than optimum light (Fig. 2B), but the short-term uptake of glycine over-high light was not lower than optimum light (Fig. 4A,B), which may indicating that glycine Scientific RepoRts | 6:21200 | DOI: 10.1038/srep21200 metabolism is critical for its contribution over long periods. The glutamine synthetase (GS)/glutamate synthase (GOGAT) cycle is considered the major pathway for N assimilation and regulation of nitrogen metabolism in higher plants 41 . Reduced glutamine synthetase activity is important for controlling photosynthetic responses to high intensity light in leaves 42 . This phenomenon is consistent with the relatively low GS activity that was observed in plants under over-high light intensity in the present study. These findings are further supported by the fact that amino acid uptake significantly decreases after exposure to the GS inhibitor methionine sulphoximine (MSX) 43 . Thus, glycine metabolism may be the rate-limiting step for glycine absorption in plants under high intensity light.
Although there is broad consensus that plants absorb organic N, little is known about the metabolism and distribution of amino acids following uptake, and the regulation of these processes 36,43 . A previous study showed that glycine taken up by the roots was primarily metabolized via transaminase reactions, with partial 15 N labelled glycine being detected in xylem sap 44 . In contrast, another study showed that glycine-N was probably metabolized by deamination in roots. Specifically, while U-13 C, 15 N-glycine was not detected in xylem sap 36 . The circulation of GS-GOGAT is also important way for organic nitrogen metabolism. For instance, amino acids taken up by ryegrass are transformed to other amino acids by transamination, with the generated ammonium being assimilated to free amino acids by GS-GOGAT 45 . Persson suggested that alanine taken up by Scots pine (Pinus sylvestris L.) root is transformed to other amino acids by alanine aminotransferase or Ala-2-oxo acid transaminase 43 . Unfortunately, we were unable to clarify this phenomenon here. However, based on the concentration of amino acids and ammonium in the shoots and roots, we detected major differences in serine and ammonium concentrations in the shoots and roots of pakchoi under optimum or over-high light conditions. These results may provide week evidence for the coexistence of the two pathways.
Several studies have shown that plants take up amino acids at relatively high rates. Thus, it is useful to investigate the bottlenecks for plant growth performance on amino acids 43 , and to study the bottlenecks under various environments. Amino acid uptake is regulated by the root concentrations of ammonium, with uptake declining after exposure to GS inhibitor MSX 43 . Our results were consistent with this previous study, where we showed that higher concentrations of ammonium were correlated with lower GS activity at the highest light intensity. Higher ammonium and lower GS activity may indicate that ammonium metabolism is a limiting step at over-high light intensity. Thornton detected 15 N in amino acids derived from 15 N-Glycine by gas chromatography-mass spectrometry, showing that uptake is the limiting step at low temperatures, while the metabolism of glycine to serine is the limiting step at high temperatures 17 . The sum of amino acids and ammonium at over-high light intensity was similar or higher than that under optimum light. This result showed that uptake is not the limiting step at over-high light intensity, supporting the short-term uptake results. At over-high light intensity, serine and glycine concentrations in the shoots and roots were lower than those under the optimum light intensity. This result indicates that light had little effect on the transamination of glycine, and the accumulated ammonium may come from deamination of glycine. Proline is a signaling molecule that regulates mitochondrial functions and cell proliferation or death, and is critical in the process of plant stress adaptation and recovery, signal transduction, and removal of free radicals, which are essential processes for plant recovery from stress 46 . High proline content in seedlings at over-high light intensity may with adaptation to light stress, showing that 540 μ mol m −2 s −1 light was too great for pakchoi growth.
Significance of studying the influence of light intensity on N uptake. In our 25-d absorption test, the contribution of different N forms to total N differed greatly among tested light intensities. The contribution of glycine to total N ranged from 18% to 29%, indicating that light intensity had a significant effect on the relative uptake of different N forms, in addition to its influence on plant growth and total N uptake. However, previous studies placed limited focus on the lighting conditions of test environments during short-term N absorption tests and controlled-condition tests, and many fail to provide clear descriptions about lighting conditions 5,9,18,47 . Furthermore, the optimum light intensity for N uptake varies among species, with the uptake of different N forms changing greatly. Lighting conditions that differ from the optimal light intensity may result in the overestimation or underestimation of the contributions of glycine-N to total N in short-term tests. Furthermore, differences in the lighting conditions used for short-term tests make it difficult to compare the contributions of glycine-N to total N across studies.
It is worthwhile to study and predict the environmental factors that influence N uptake and plant growth, to enhance ecosystem research and management. Since the climate is changing greatly with the effect of global warming, greater focus should be placed on the effect of environmental change, allowing greater flexibility in managing ecosystems, especially the agriculture ecosystem. Although the effects of pH 48,49 , light intensity 11 , and temperature 50 on the uptake of ammonium, nitrate, and/or amino acids have been studied independently, the interaction of these factors has received limited research. Thus, general models to predict the compounding effects of environmental factors are needed in the face of the future climate change.

Methods
Pakchoi (Zhebai 6) was cultivated in a sterile environment. Pakchoi seeds were soaked for 12 h in purified water, and sterilized as described by Wu et al. 51 . The seeds were then sown in sterilized culture dishes containing two layers of moist cotton gauze at the bottom and this was defined as day 1 of each experiment. The culture dishes were placed in a sterilized culture room, with a photoperiod of 12 h and light intensity of 380 μ mol m −2 s −1 provided by fluorescent lamp, a day/night temperature of 25/20 °C and humidity of 60%/40%. Three days after germination, seedlings grew to approximately 1 cm length. Seedlings were transplanted to 50 mL centrifuge tubes (filled with 0.5% cooling-off agar) with small holes in the caps. After 2 days, the seedlings had completely grown out of the holes and the holes were sealed with silicone rubber (Nanda 704). One day later, the seedlings were transferred together with the centrifuge tube cap to a new centrifuge tube (covered with silver paper to avoid any effects of Scientific RepoRts | 6:21200 | DOI: 10.1038/srep21200 light on root growth), which was filled with nutrient solution. The nutrient solution contained 4 mM CaCl 2 , 2 mM K 2 SO 4 , 2 mM KH 2 PO 4 , 1.4 mM MgSO 4 ·7H 2 O, 0.1 μ M NaMoO 4 ·2H 2 0, 0.4 μ M CuSO 4 ·5H 2 O, 1 μ M ZnSO 4 ·7H 2 O, 8 μ M H 3 BO 3 , 10 μ M MnCl 2 , 5 μ M Na 2 EDTA, and 18.3 μ M FeSO 4 ·7H 2 O, and the pH was adjusted to 6.2. N mixtures prepared for each experiment were added to the nutrient solution before use. The nutrient solution and all materials, except seeds and N solution, were sterilized by steam under high pressure (121 °C for 30 min). The N solutions were passed through a 0.22 μ m membrane filter (Millipore, PES Membrane, Ireland) prior to combining with the nutrient solution. The nutrient solution was changed every 3 days during experiments in a clean bench. Experiment 1: Effect of light intensity on pakchoi growth, photosynthesis, and relative uptake of nitrate, ammonium, and glycine. Three mixtures of NO 3 − , NH 4 + , and glycine (1:1:1) were prepared.
In each mixture, a different N source was labelled with 15 N (50.22% Na 15 NO 3 − , 50.17% ( 15 NH 4 ) 2 SO 4 or 50.16% 15 N-glycine). Each N mixture was tested separately. N mixtures were added to the nutrient solution for a total N concentration of 3 mM. Light intensity was controlled by relative distance from the fluorescent lamp, and plants received 36,162,288,414, and 540 μ mol m −2 s −1 light. There were 15 treatments in total (5 light intensity treatments × 3 N mixtures) and 6 pakchoi seedlings were subjected to each treatment. In addition, we created two "blank" seedlings for one treatment by providing it with unlabelled N mixture at the same concentration as for treated plants. After 22 days, 9 pakchoi seedlings were randomly selected from each light intensity treatment. Then the photosynthetic characteristics were measured on fully expanded leaves using a portable open-flow gas exchange system Li-6400 (LI-COR Biosciences, Lincoln, NE, USA). The concentration of CO 2 was 385 μ mol L −1 , and the light intensity was 1000 μ mol m −2 s −1 . On day 25, the pakchoi seedlings were destructively sampled. Based on the results of experiment 1, 360 μ mol m −2 s −1 was the optimal light intensity for glycine uptake by pakchoi, whereas 90 and 540 μ mol m −2 s −1 were too low and too high for pakchoi growth, respectively.

Experiment 3: Effect of light intensity on the short-term uptake of glycine. Experiment 2 showed
that light intensity has a strong effect on glycine uptake, raising the question of whether this phenomenon was caused by uptake. Pakchoi seedlings were pre-cultivated for 25 days in a sterile environment as in experiment 2. After pre-cultivation, 36 similar pakchoi seedlings (6 for each treatment) were selected, and the roots and centrifuge tubes were washed several times with purified water. The seedlings were "hungry" cultivated in sterilized nutrient solution without N for 1 night (approximately10 h), after which a short-term absorption test was performed. Eighteen pakchoi seedlings were cultured with 3 mM-labelled glycine (98.10% 15 N) under 90, 360, and 540 μ mol m −2 s −1 light for 4 h.
The effects of light intensity on the active and passive absorption of glycine were also examined by using carbonyl cyanide 3-chlorophenylhydrazone (CCCP) 52 at the same time. Eighteen "hungry" cultivated pakchoi seedlings were then pre-treated with 50 μ M CCCP for 1h, and cultured with 98.10% 15 N glycine under 90, 360, and 540 μ mol m -2 s −1 light for 4 h. The 15 N in CCCP-treated plants was the result of passive uptake.
Roots and shoots in experiment 1, 2, and 3 were harvested separately, and pairs of seedlings from each treatment group were combined to form single samples. To remove 15 N on root surfaces, roots were washed by ultrasonification in sterile water, followed by 50 mM CaCl 2 , and several washes with purified water. The roots and aboveground parts were freeze-dried (Labconco Freezen System, USA) and ground to a fine powder with a ball mill (Retsch MM301, German). The N content was determined by the Micro-Kjeldahl method (Yihong, NKD6200), titrated with 0.05 mM sulphuric acid. The titrated solution was condensed by rotary evaporator (EYELA, SB-1100) at 55 °C until N concentration was greater than 0.5 mg mL −1 . The 15 N enrichment of the condensed solution was determined using a Tracer MAT-271 (Finnigan MAT, USA).

Experiment 4: Effect of light intensity on the activity of glycine metabolic enzymes. Experiment 2
showed that long-term glycine uptake in 90 and 540 μ mol m −2 s −1 was significantly lower than 360 μ mol m −2 s −1 light; however, in experiment 3, the short-term uptake of glycine in 540 μ mol m −2 s −1 light was not lower than that in 360 μ mol m −2 s −1 light, raising the question of whether the metabolism of glycine inhibits the N contribution of glycine. Pakchoi seedlings were pre-cultured for 25 days as described in experiment 2. Eight similar size seedlings at each level of light intensity were selected, and the roots were washed several times with purified water. The seedlings were "hungry" cultivated for 1 night, then cultivated with 3 mM glycine for 4 days, and the nutrient solution was changed every 2 days. Glycine was supplied at a concentration of 3 mM to provide an equimolar concentration of N with experiment 1, while excluding the influence of other N forms on the activity of enzymes. The activities of glutamine synthetase (GS) 53 , glutamic-pyruvic transaminase (GPT), and glutamic oxalacetic transaminase (GOT) 54 in the roots and leaves were measured. Over an extended period, biomass may differ significantly among groups, which may affect the enzyme concentrations in plants because of the "magnification effect" or the "dilution effect. " Therefore, we subjected the seedlings to different light intensities for just 4 days. In this way, the biomass of seedlings did not vary significantly among treatment groups (data not shown), allowing us to conclude that any differences in enzyme activity were due to the effects of light intensity rather than to biomass. Scientific RepoRts | 6:21200 | DOI: 10.1038/srep21200 Experiment 5: Effect of light intensity on the metabolism of amino acids. Experiment 4 showed that glutamine synthetase in 540 μ mol m −2 s −1 light was significantly lower than that in 360 μ mol m −2 s −1 light; thus, the limiting step of amino acid metabolism was investigated here. Pakchoi seedlings were pre-cultured for 25 days, as described in experiment 2. Twenty-four similar size seedlings at each level of light intensity were selected, and the roots were washed several times with purified water. The seedlings were "hungry" cultivated for 14 h. Then, the seedlings were cultivated with 3 mM glycine under 360 and 540 μ mol m −2 s −1 light for 12 h. Subsequently, the roots and shoots of pakchoi were harvested separately, and 4 seedlings from each treatment group were combined to form single samples. To remove glycine from the root surfaces, roots were washed by ultrasonification in sterile water, followed by 50 mM CaCl 2 , and several washes with purified water. Further, 1 g aliquots of fresh shoots or roots were ground with 4 ml 5% sulphosalicylic acid, and left to stand for 1 h. The solution was centrifuged at 14000 g for 10 min. The supernatant was retained and passed through a 0.2 μ m membrane filter. Amino acid content was detected by an automatic amino-acid analyser (L-8900, Hitachi, Japan).
Calculations and statistics. The uptake of different N sources was determined by the 15 N concentration in treated seedlings relative to the 15 N concentration in "blank" seedlings not provided with labelled N. The amount of NO 3 − , glycine, and NH 4 + taken up from the labelled N was calculated using equation 1 55 where N uptake is the amount of a given N source taken up into the roots or shoots of pakchoi seedlings; N Total-N is the total N content of the roots or aboveground parts; A s is the 15 N atom% in the roots or aboveground parts; A c is the 15 N atom% in the "blank" that was supplied with unlabelled N mixture in the shoot of experiment 1 and the shoot or root of experiment 2; A f is the 15  where N contribution is the proportion of total N taken up as glycine, NO 3 − or NH 4 + by whole pakchoi seedlings; N uptake is the amount of a given N source taken up into the roots or shoots of pakchoi seedlings, as calculated from equation (1); and N total-N is the total N total mass of N contained in pakchoi seedlings. where Tr is the transportation rate; N shoot is the amount of glycine-N in the shoot; and N (shoot + root) is the total glycine-N in the root and shoot, as calculated by equation (1).

Statistical analysis.
All statistical analyses were performed using SAS 8.2. Data are presented as the mean ± standard error (SE). One-way analysis of variance (ANOVA) was used to evaluate differences between treatments and differences in mean values were tested using Duncan's multiple range method (p < 0.05). Figures were created using Origin 8.1.