Table 2 Kinetic parameters of GTF-D wild-type and mutant M4 enzymes.

From: Engineering a Carbohydrate-processing Transglycosidase into Glycosyltransferase for Natural Product Glycodiversification

   Km (mM) kcat(min−1) kcat/Km(min−1mM−1)
Catechin WT 3.60 ± 0.44 546.35 ± 51.48 151.76
M4 2.84 ± 0.37 738.15 ± 54.95 259.91
Genistein WT n.d. n.d. n.d.
M4 1.47 ± 0.15 46.52 ± 2.41 31.65
  1. n.d. not determined.