The effect of demineralized bone matrix on bone growth into porous disks.
Disks were implanted on calvaria of athymic nude rats with and without DBX and bone-to-implant contact was assessed after 2, 5 and 10 weeks. MicroCT cross-sectional images show sintered disks ((A), rough) implanted on calvaria of rats without ((A), rough) or with ((A), DBX) demineralized bone matrix after 10 weeks, which black indicating the implant and white indicating new bone formation. These images were analyzed for bone-to-implant contact (B) and bone ingrowth in holes (C). MicroCT top down images, thresholded to remove underlying calvaria, revealed new bone growth into pores of the disk (D). Histomorphometric analysis was conducted per implant to evaluate bone-to-implant contact (E) and bone ingrowth in holes (F). Histological images depict bone growth along sides of disk pores for both groups (G). MicroCT and histological image scale bars represent 1 mm. One-way ANOVA with Bonferroni analysis, p < 0.05, * vs. all groups (B,C), * vs. 2 week rough, # vs. 2 week DBX, $ vs. 5 week DBX (E,F).