Figure 6 | Scientific Reports

Figure 6

From: Extracellular matrix stiffness dictates Wnt expression through integrin pathway

Figure 6

Wnt/β-catenin pathway contributes to ECM stiffness regulating chondrocytes phenotype maintenance.

(a) Chondrocytes were cultured on stiff or soft ECM for seven days in the presence of 10 μM Cardamonin or DMSO, followed by determination of Sox9, Aggrecan and ColI expressions by immunocytochemical staining. Scale Bar: 30 μm. (b) represents the statistical results of (a). (c) Chondrocytes were cultured on stiff or soft ECM for seven days in the presence of 10 μM Cardamonin or DMSO, followed by determination of Aggrecan, ColII and ColI expressions by western blotting. (d) Chondrocytes were cultured on stiff or soft ECM for seven days in the presence of Wnt1 (100 ng/ml) or solvent, followed by determination of Aggrecan, ColII and ColI expressions by immunocytochemical staining. Scale Bar: 30 μm. (e) represents the statistical results of (d). Scale Bar: 30 μm. Results on fluorescence intensities from 6 independent experiments on stiff matrix are expressed as percentages (mean ± SEM) of the corresponding results on the soft matrix. Western results were from 3 independent experiments, with blots exemplifying one experiment and the bar graphs showing the combined results on stiff matrix expressed as percentages (means ± SEM) of the corresponding results on soft matrix. GAPDH was used to normalize for equal loading in western blotting. *P < 0.05, **P < 0.01, ***P < 0.001, n.s. stands for not statistically significant.

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