Figure 4 | Scientific Reports

Figure 4

From: Extracellular matrix stiffness dictates Wnt expression through integrin pathway

Figure 4

ECM regulated β-catenin pathway and Wnt expression by Integrins.

Results from primary chondrocytes 48 hr after seeding on stiff (100 kPa) or soft (0.5–1 kPa) ECM. (a) Phosphorylated GSK3β levels, (b) β-catenin and (c) Wnt1 levels in the presence of 20 μg/ml β1 integrin blocking antibody or control antibody were analyzed by western blotting. (d) Phosphorylated FAK levels in the presence of 20 μg/ml β1 integrin blocking antibody or control antibody were analyzed by western blotting. (e) β-catenin and (f) Wnt1 levels in the presence of integrin β1 siRNA or scramble were analyzed by western blotting. (g) β-catenin in the presence of integrin α1 siRNA or scramble were analyzed by western blotting. (h) β-catenin levels in chondrocytes 48 hr after seeding on stiff or soft ECM in the presence of integrin α10 siRNA or scramble were analyzed by western blotting. Western results were from 3 independent experiments (except 4 in b, 6 in c), with blots exemplifying one experiment and the bar graphs showing the combined results on stiff matrix expressed as percentages (mean ± SEM) of the corresponding results on soft matrix. GAPDH was used to normalize for equal loading in western blotting. *P < 0.05, **P < 0.01, n.s. stands for not statistically significant.

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