Self-Styled ZnO Nanostructures Promotes the Cancer Cell Damage and Supresses the Epithelial Phenotype of Glioblastoma

Extensive researches have been done on the applications of zinc oxide nanoparticles (ZnO-NPs) for the biological purposes. However, the role and toxicity mechanisms of ZnO nanostructures (ZnO-NSts) such as nanoplates (NPls), nanorods (NRs), nanosheets (NSs), nanoflowers (NFs) on cancer cells are not largely known. Present study was focused to investigate the possible mechanisms of apoptosis induced by self-designed ZnO-NSts, prepared at fix pH via solution process and exposed against human T98G gliomas including various cancers and non-malignant embryonic kidney HEK293, MRC5 fibroblast cells. NSts were used for the induction of cell death in malignant human T98G gliomas including various cancers and compared with the non-malignant cells. Notably, NRs were found to induce higher cytotoxicity, inhibitory effects on cancer and normal cells in a dose dependent manner. We also showed that NRs induced cancer cell death through oxidative stress and caspase-dependent pathways. Furthermore, quantitative and qualitative analysis of ZnO-NSts have also been confirmed by statistical analytical parameters such as precision, accuracy, linearity, limits of detection and limit of quantitation. These self-styled NSts could provide new perception in the research of targeted cancer nanotechnology and have potentiality to improve new therapeutic outcomes with poor diagnosis.

Scientific RepoRts | 6:19950 | DOI: 10.1038/srep19950 of cancers, brain, lung and human thyroid carcinomas cancer are commonly affected and considered as one of the main reason for cancer deaths. The symptoms of lung cancer are caused in the patients by primary tumor (metastasis) formation in the form of cough, chest pain, haemoptysis, dyspnea and recurrent pneumonia or bronchitis 20,21 . Towards this area, it has been shown that lung cancer can be successfully reduced via the utility of nanostructured materials due to the role of nanoparticles as a drug delivery carrier which reduces the nonspecific toxicity of potent anticancer drugs 22 . Higher tumor malignancy could be frequently deteriorated after cancer treatment procedures. To overcome these problems, several therapies, such as chemotherapy, radiotherapy, immune therapy, etc have been implemented to protect the cancer but the success rate of therapeutic outcomes is still not up to date 3,4 .
The present work demonstrates the effects of ZnO-NSts such as NPls, NRs, NSs and NFs structures against various human cancer cells. The determination of nanostructures interaction with cancer cells were also demonstrated by illustration and statistical analytical parameters. These nanostructures were used as a target material for inducing cell death (apoptosis) in malignant cells. Here, we uncover that these NSts have the potential to induce genetic damage for cancer cell death. Interestingly, these NSts significantly reduced the motility of aggressive cancer cells. These findings proposed that targeted NSts could improve existing therapeutic approaches in field of cancer nanotechnology.

Results
Crystalline and morphological studies of ZnO-NSts synthesized at optimized pH. The general morphology of the NSts (NPls, NSs, NRs and NFs) was analyzed using FE-SEM prepared at desired pH conditions with the use of precursor solution of zinc acetate dihydrate and sodium hydroxide. The aqueous solution of zinc acetate dihydrate and sodium hydroxide have been calibrated and optimized at pH (6, 9, 10, and 12) with the incorporation of H + and OH − ions (HCl & NaOH). Figure 1A,B shows the FESEM images of NPls and NSs composed with tiny NRs structure optimized at pH 6 and 7. A visible change has been observed in the morphology of ZnO-NSts with the addition alkali (NaOH) solution. As the solution gets basic OH − ions from sodium hydroxide, the sheet changes to NRs (Fig. 1C) and further changed to primary flower shaped-like structure. At pH 12, the structure gets more stable and shows a complete flower shaped morphology (Fig. 1D). The diameter of each NR is ~150-200 nm whereas length is about 2-4 μ m long, sharp tipped with hexagonal surface.
These NSts were further investigated by TEM microscopy at 200 kV. Figure 1E shows the low magnification TEM image of ZnO-NFs. The observations from TEM are closely related with the FE-SEM observation and clearly consistent. Figure 1B shows that the size of each NR is exactly identical dimension (~200 nm long and full array was about 2-4 μ m) obtained from FE-SEM. Figure 1F shows the HR-TEM (high resolution transmission electron microscopy) image of ZnO-NFs composed with hexagonal nanorods. From the HR-TEM image, we can easily observe that the distance of lattice fringes between two adjacent planes are ~0.526 nm, which is almost identical to the lattice constant of wurtzite ZnO. The observed lattice distance from HR-TEM image, again indicates that the obtained NR of flower shaped morphology exhibit wurtzite hexagonal phase and are preferentially grown along the c-axis [0001] as shown in Fig. 1F.
Analytical determinations of ZnO-NSts. The analytical performance was measured for the synthesized NPls, NSs, NRs and NFs formed at fixed pH, which are directly depends on the range of concentration of nanostructures, calculated by mathematical or statistical analytical tools as seen in Fig. 2. In the suspension solution of NSts with cells, statistical approach was applied for the appropriate choice and evaluated the capability of pH mediated NSts of ZnO and their fitness for the purpose of inhibition of growth of cancer cells. The analytical determination was studied with different shaped ZnO-NSts.
The purposed method was based on UV-visible ZnO-NSts (NPls, NSs, NRs and NFs) spectrophotometer, which is a best tool and gives quantitative and qualitative result established by valid statistical analytical techniques under the guideline of ICH. The absorption spectra were recorded for the different shaped ZnO-NSts such as NPls, NSs, NRs and NFs etc. At initial pH 6 (grown plates), at neutral pH 7 (sheet-like structure), pH 10 (rod-like structure) and pH 12 (flower like structure) at 600 nm wavelength and the graph can be seen in Fig. 2A-D. The calibration graph was made for the ZnO-NSts (NPls, NSs, NRs and NFs) at optimized pH by plotting absorbance against concentrations (0.5-2.2 μ g/mL), which gives linearity as can be seen in Fig. 3A-E. The molar absorptivity (є) of the ZnO-NSts (ZnO-NFs-T98G = 0.915 × 10 2 , ZnO-NFs-SNU-80 = 2.0 × 10 2 , ZnO-NFs-H460 = 2.50 × 10 2 , ZnO-NFs-HEK = 2.70 × 10 2 and ZnO-NFs-MRC-5 = 2.70 × 10 2 L mol -1 cm -1 of the resulting colored sample solution indicates high sensitivity of ZnO-NSts towards cancerous cells (Table S1, supporting information). The detection limit (LOD) and quantitation limit (LOQ) was determined for known concentration of sample solution at minimum level, their empirical formula provided more reliably values to ensure that they are fit for the proposed method and confirmed this method is accurate, precise, reproducible and gives adequate results. The relative standard deviation (RSD), standard analytical error (SAE), Confidence limit at 95% confidence level (n= 5) results are summarized in Table S2, supporting information. It is evident from these observations that the relative standard deviation (RSD %) in the ranges from (0.32-1.69%) is accurate and satisfactory 23-25 . Optimization and validation of the proposed method. The optimization and validation of suspension solution or analytes (ZnO-NSts) was recorded against in terms of many factual variables factors such as concentration, volume, pH, time and temperature etc, which is a common way for the analytical method that gives standardization of sample. The validation methods and their parameters clearly define the rule and regularities for the development of analytes, quality control, and each individual required the analytical data of analytes. The concentration of used ZnO-NSts (NPls, NSs, NRs and NFs) at optimized pH, are applied for to control the growth of cancer cells under optimized and validated experimental condition. The analytical procedure Scientific RepoRts | 6:19950 | DOI: 10.1038/srep19950 (within a given range from 0.5-2.2 μ g/mL) has ability to obtained test results, which are directly proportional to the concentration of analyte in sample solutions. Beer's law was obeyed for the obtained concentration range from 0.5-2.2 μ g/mL therefore, the fixed concentration range for plates to flower like structure as can be seen in Fig. 3A-E. The regression analysis of calibration data obtained with the help of absorbance verses concentration, which gives the value of correlation coefficient (ZnO-NFs-T98G = 0.994, ZnO-NFs-SNU-80 = 0.994, ZnO-NFs-H460 = 0.992, ZnO-NFs-HEK293 = 0.995 and ZnO-NFs-MRC-5 = 0.996 at pH 12) with apparent molar absorptivity (є) (ZnO-NFs-T98G = 0.915 × 10 2 , ZnO-NFs-SNU-80 = 2.0 × 10 2 , ZnO-NFs-H460 = 2.50 × 10 2 , ZnO-NFs-HEK293 = 2.70 × 10 2 and ZnO-NFs-MRC5 = 3.20× 10 2 L/mol/ cm respectively. The detection limits (LOD) and quantitation limit (LOQ) were found to be 0.172: 0.523 for ZnO-NFs-T98G, 0.176:0.535 for ZnO-NFs-SNU-80, 0.213:0.645 for ZnO-NFs-H460, 0.170:0.517 for ZnO-NFs-HEK293 and 0.146:0.443 for ZnO-NFs-MRC-5 μ g/mL respectively. To evaluate intra-day and inter-day precisions, analysis for ZnO-NFs at three concentration levels (0.5, 1.5 and 2.0 μ g/mL) were carried out within the same day and five consecutive days. The intra-day and inter-day RSD values ranged from pH 12 ZnO-NFs-T98G for (0.60-1.  (Table S2, supporting information). The RSD (%) obtained were quantitative for pH 12 indicating the good accuracy of the proposed method analyzed by spectrophotometric method [23][24][25] .

Inhibitory effects of ZnO-NSts on cancer and normal cells.
In this study, we have compared four different types of ZnO-NSts (ZnO-Pls, ZnO-NSs, ZnO-NRs, ZnO-NFs) on three malignant and two non-malignant cell lines. To check the effectiveness of ZnO-NSts, we have treated both normal and cancer cells by different concentrations of NSts and their cytotoxicity was assessed using MTT proliferation assay. The MTT cell proliferation assay has been widely accepted as a reliable way to measure the cell proliferation rate and conversely when metabolic events lead to apoptosis or necrosis. Table S3 (supporting information) shows the IC 50 values of NSts on the T98G brain cancer, H460 lung cancer, SNU-80 thyroid cancer, non-malignant HEK kidney and MRC-5 lung fibroblast cell lines. The data obtained by MTT assay showed that all NSts have inhibitory effects on the growth of cancer cells in a dose-dependent manner. All NSts effectively inhibited the growth of T98G, H460, SNU-80, HEK293 and MRC-5 cells, with their IC 50 values ranging from 38.26 to 185.93 μ g/mL. All NSts were found to inhibit 50% T98G glioma cell growth in the range from 36.80-48.62 μ g/mL after 24 hr treatment. Interestingly, our data indicated that all NSts were found to be less cytotoxic to non-malignant normal HEK293 and MRC5 cells at moderate concentrations (187.5-11.71 μ g/mL) as shown in Table S3, supporting information. IC 50 values of the NSts for HEK293 and MRC-5 cells were in the range from 86.62-185.15μ g/mL. As we mentioned earlier, that MTT assay shows that T98G cells were severely affected by the NSts treatment as dose-dependent manner. These experiments indicate that inhibition of T98G cancer cell growth by NSts may be due to either growth arrest or cell death. Collectively, these results clearly demonstrate that all NSts are effective to block cancer cell growth but more prominent effect was seen by ZnO-NRs exposure on cancer cells rather than on non-malignant cells, which may be by increasing the mitotic or interphase death. Figure 4A-C shows the growth kinetics of the T98G cells. Data obtained by growth kinetics assay showed that the all NSts have an inhibitory effect on T98G cells that is dependent on exposure time. The cells were treated at 11.71 to 187.50 μ g/mL concentrations of nanostructures. Cell suspensions were made in media layers of 4 mm thickness in 6 well plates. As a note, the cells exposed to ZnO-NSs treatments show less effect than those exposed to a ZnO-NRs treatment. The maximum effect was seen after ZnO-NRs exposure, by inhibiting the cell growth from 42-96% at 72 h at all 5 concentrations. Whereas in case of ZnO-Pls exposure on T98G cells, we found that 29.87-98.49% cells were died and their viability was 1.51-70.13%, at 72 hr respectively after treatment. ZnO-Pls showing incubation time dependent effect on cells, maximum effect is obtained only at after 48 h however showed no such effect before 48 hr of incubation. However ZnO-NFs is also showing incubation time dependent effect on the growth of T98G cells and having maximum inhibitory effect only at 48 hr of incubation rather than 24 or 72 h after incubation. Overall, all nanostructures inhibits the growth of T98G cells in a dose and incubation time dependent manner. These experiments indicate that NSts inhibit T98G cancer cell growth which may be due to either growth arrest or mitotic cell death. Figure 4D shows the effect of NSts on the colony forming capacity of T98G brain cancer cells. To this end, we performed a clonogenic assay to confirm the colony formation inhibition results due to NSts treatment. Only a fraction of seeded cells retains the capacity to produce colonies after cytotoxic compound treatment. Clonogenic assay or colony formation assay is an in vitro assay based on the ability of a single cell to grow into a colony. This in vitro clono-genicity method was recognized as a valid surrogate assay for tumor growth in vivo. As NSts concentration increases, the treatment enhances cell death and inhibits the colony formation capability of the T98G cell population. Our data shows that all NSts are capable of inhibiting the colony formation and survival of exponentially growing T98G cells in concentration-dependent manner, after treatments with different concentrations (11.71-93.75 μ g/mL) as evidence by the reduction in the number of colonies formed (Fig. 4D). It was observed in the Fig. 5A-C, that the survival fraction of T98G cells has been drastically decreased after NSts treatment. Even at low dose, 11.71 μ g/mL exposures of ZnO-Pls, ZnO-NSs, ZnO-NRs and ZnO-NFs shows significant decline in colony survival and their % colony survival was found to be 71.77, 65.32, 57.25 and 76.61 respectively. However, the significant drastic decline in their colony survival percentage could be observed after exposure to 23.43 μ g/mL concentration of nanostructures. ZnO-Pls and ZnO-NRs show higher colony formation inhibitory effect on T98G cells, at all doses. Remarkably, ZnO-NRs showed maximum inhibition at doses 11.7, 23.4, 46.8 and 93.7 μ g/mL exposures with percentage colony survival 57.25, 50.80, 8.87 and 0 respectively. Collectively, these results indicate that these NSts treatments are efficiently inhibiting the clonogenicity of glioma cells at all the effective concentrations. Nanostructure accumulated intracellular ROS is essential factor for cell death. Next we sought to check the factors which are responsible for NSts induced cell growth inhibition and observed cell death. For this purpose, first we checked the ROS formation inside the cells. Figure 5 shows ROS measurement in NSts treated T98G brain cancer cells. The decreased level of MTT formazan with growth inhibition and increased level of clonogenic inhibition might lead to increased oxidative stress that would compromise the survival fraction leading to cell death in T98G cells. Here, we found that all NSts enhanced ROS production at 24 hr inside the T98G cells. Treatment by NPls, NSs, NRs and NFs increases the DCF fluorescence by 47.38% (MFI-130), 45.96% (MFI-128), 87.38% (MFI-170) and 53.64% (MFI-136) respectively at 1.46 μ g/mL concentration (Fig. 5A,B), when compared to untreated control (MFI-83.6).Notably, maximum effect was seen by ZnO-NRs in T98G cells. To further confirm this effect, we treat the T98G cells by NRs in the presence of well-known ROS inhibitor, NAC (10 μ M, N-acetyl L-cysteine, Sigma Aldrich). NAC is a antioxidant and prevents cell apoptosis or death induced by reactive species. NAC is added 4 hour before nanostructure exposure to cell culture. As expected, addition of NAC significantly (p< 0.05) blocked the ROS generation in ZnO-NRs treated T98G cells which clearly indicate that ROS accumulation is necessary for NSts induced cell death effect (Fig. 5C).
Caspases plays a crucial role in nanostructures induced apoptotic cancer cell death. Next, to assess the effect of NSts on the activity of Caspase-3/7 in brain cancer cells, we treated the T98G cancer cells with all NSts at a dose of 1.46 μ g/mL. It is broadly accepted that the apoptotic cell death of mammalian cells is closely associated with activation of executioner caspases which has important roles as early biochemical markers for apoptosis. To detect the caspases, we used Caspase-Glo ® 3/7 assay kit (Promega) that uses a luminogenic substrate containing the DEVD sequence which is selective for caspase-3 and -7. All detection steps were performed using as manufacturer's protocol. Following 24 hr NSts treatment, the caspases activity in both untreated and treated cancer cells were measured using luminometer as shown in Fig. 6A. Caspases activity level detected in untreated control cells corresponded to the portion of apoptotic cells present in the naturally growing population due to natural aging. It is worth-mentioning here that ZnO-NRs show the highest caspases activity among all the NSts treated in T98G cancer cells. To confirm further the potential involvement of caspases in observed cell death by nanostructures, we pretreated the T98G cells with pan-caspase inhibitor Z-VAD-FMK (100 μ M, R & D To extend our observations, we next investigated that NSts induced cell death is apoptotic or not, we checked apoptosis-related gene expression such as caspase 3 (CASP3), caspase 9 (CASP9), p53, poly ADP ribose polymerase (PARP), cytochrome C (CYTS) by real time PCR analysis. Notably, our results show that all above genes were enhanced after ZnO-NRs treatment. Interestingly, CASP3 (executioner caspases) was much elevated after ZnO-NRs treatment, signifying its main role in induced apoptotic cell death in cancer (Fig. 6C). These findings suggest that the NRs treatment may induce apoptosis through the caspase-dependent pathway.
When ZnO-NRs treated T98G cells morphology was examined using immunofluorescence, observed that after NRs exposure the surviving cells adhered to the surface show that actin fibers became shortened and thinner in T98G cells and nucleus become enlarged and swollen and was fragmented (Fig. 7A). The enlarged images indicated that actin fibers became shortened and localized near cell membrane. To look further closely into the nucleus, we performed micronucleus (MN) assay after all NSts treatment. Maximum micronuclei were formed after ZnO-NRs treatment (Fig. 7B). However, another NSts exposure also revealed the increased micronuclei formation in exposed T98G cells. Based on above results, we conclude here that caspases induced PARP cleavage resulting DNA damage is crucial for NSts induced apoptotic cell death.

Nanostructures suppresses the invasiveness of glioblastoma through EMT.
In line with agreement of above consisting results, we next determined that which mechanism is involved in NSts effect as a cancer suppressor. The epithelial-to-mesenchymal transition (EMT) is the process in which epithelial cells change their cell polarity and adhesion property and increase their invasiveness and migration capacity. Invasiveness is a characteristic feature of aggressive cancers, and this intricate property can be considered as an intense investigation for some time. An emerging concept suggests that EMT phenomenon involved in cancer progression 26 and has been considered to regulate invasiveness of cancer cells at the tumor-stroma interface 27 . Based on these studies, we thought to gain insight in the process of EMT in glioma cells after NRs exposures and characterized the migratory and invasive properties. We observed that ZnO-NRs significantly (p< 0.05) decreased the migration and invasion in glioma cells (Fig. 8A). However, various molecules are involves in EMT process, we checked neuronal cadherin (N-cadherin) and transcription factor zinc finger E-box-binding homeobox1 (Zeb-1) which is mostly activated during EMT process. Staining and gene expression analysis clearly shows that these markers are significantly attenuated after ZnO-NRs treatment in glioma cells (Fig. 8B,C). Taken together these results suggest that NRs has the potential to suppress the epithelial phenotype in cancer cells to inhibit the cancer progression.

Discussion
In the family of metal oxides, ZnO which has wide verity of nanostructure such as nanoparticles, nanorods, nanowire, nanobelts, nanonuts, nanotube, nanoring, nanobridges, nanonails, nanocubes, nano sheet, sea-urchin, nanoflowers and nanofan shaped nanostructures, and the shape evolutions strongly depend on the pH value during growth of NSts [28][29][30][31][32][33][34][35] . All these nano and micro-structures were prepared either by low temperature synthesis or thermal evaporation methods 36 . The nano& micro scaled nanoproducts exhibit wide applicability in various industrial and biomedical applications such as drug delivery, DNA damage, cytotoxicity, cancer etc; due to their high surface area to volume ratio, which enhances wide catalytic properties 37 . Here, we have described the utility of diverse shaped NSts such as NPls, NSs, NRs and NFs in growth inhibition of cancer cells. The obtained nanoscale structures were well characterized with FE-SEM, TEM equipped with the HR-TEM to know the general morphology of NSts and their crystallinity for the formation of nanostructures. The FE-SEM and TEM images, which showed that the prepared NSts having diverse shaped structure such as the early staged grown plates synthesized at pH = 6, whereas a small deviation have been seen as the pH was increased to neutral solution pH= 7. The sheet-like structure converted to aligned plate and rod like structure. As the value of zinc oxide suspension solutions pH changes from acidic to basic (6-9), grown nanosheet structures turns to separate as well as structurally arranged-nanorods (Fig. 1), but when the pH value of the solution increased to pH = 10, only rod-like structure was observed with hexagonal shape and tapered tip (Fig. 1). As the solution pH value increases to 11, NRs are seen forming a flower like structure and synthesis at pH = 12, results in a complete flower like morphology composed to sharp tipped hexagonal nanorods was formed with nearly smooth surface with an average diameter of each nanrods 150-200 nm, whereas length goes about 2-4 μ m long, sharp tipped with hexagonal surface in spherical shaped flowers structures. The high resolution TEM (HR-TEM) again confirms that the grown structures having lattice spacing (~0.526 nm), crystalline and equal to pure wurtzite phase ZnO. The NSts of ZnO is an optically active material, which exhibit similar band gap to the commercial zinc oxide powder (3.37eV) and a typical characteristic band of pure wurtzite ZnO 38 . The pH value has always been an important parameter to finalize nanostructured products for better understand (effect of pH) and their growth of different morphology of ZnO-NSts 38 and has capability to inhibit the growth of cancer and normal cells such as T98G, SNU-80, H-460 and multiplications with HEK293 and MRC-5 fibroblast cells. It is also well known that the solutions pH support to determine the quantitative, qualitative response for the prepared pH mediated NSts for instance fitness by statistical, numeric and graphical optimization tools, and facilitate their optimization of multiple responses with other parameters such as concentration, temperature and time etc. Therefore, the suspension solution of pH based nano-product appears in phase formation, particles size and morphology of nanostructures, analyzed via analytical method using UV-vis spectrophotometric technique. As evaluation of analytical method indicates excellent performance characteristics are accuracy, specificity, linearity measure, solution working range, limit of quantitation (LOQ), limit of detection (LOD), intermediate precision and reproducibility etc; validated by as per the guidelines of International Conference of Harmonisation (ICH) 24,25,[39][40][41][42][43] . The target of statistical analysis provides broad knowledge of system suitability and characteristics performance, which relates to the multi-dimensional data's operator, obtained from the process used in experimental design. Hence, this method is an adequate, accurate, precise, acceptable behavior and based on criteria of analytical study to control morphology of nanostructures. On the basis of physical morphology and characteristics, we can postulates that all the cells have small pores which helps, the NSts to enter into the inner membrane of cells. As we know that the prepared nanostructures have property to enter into cells (~20 μ m) very easily due to very small size (~150-200 nm) as compared to the cancer cells (~20 μ m) 44,45 . The high density of NSts in the liquid system strongly favors the rapid formation of agglomerates and it's assumed that these agglomerates of NSts destroy the cells 45 .The ROS is another factor, which is produced in solution of NSts with cells and are responsible to form the free radicals in the solution and these free radicals penetrates the outer wall of the cells and enter to inner wall of the membrane. These free radicals when reacts with the organelles, enzymatic changes occurred and it leads to the disorganization of the cells and cell contents. The oxidative stress plays an important role in the toxicity with NSts and suggested that the extreme generation of ROS through NSts reduces the cellular antioxidant capacity 46,47 . The phenomenon why ZnO-NSts are responsible to regulate the growth or destroy the cell organelles and their biochemical and enzymatic changes of cancer cells is under investigation and it needs further study to investigate the role of ZnO-NSts in cancer cells 45 . Here these findings shows that the nanostructured (NPls NSs, NRs, and NFs) are more potent material against cancer cells, which is may be due the small size of NSts and it can be easily entered to cell membrane 48,49 . Several published literature employed metal oxides nanostructures applied at a high concentrations for the toxicities studies, which may be difficult for the human exposure. We believe that these newly biocompatible NSts would be helpful to control the growth cancer cells at lower concentration and have no adverse effect on the body 50 .

Conclusion
The obtained results of NSts (NPls, NSs, NRs, and NFs) treatment against human T98G glioma, H-460 lung carcinoma and SNU-80 thyroid carcinoma cells were compared with non-malignant HEK293 and MRC-5 fibroblast cells. All these NSts were more efficacious on cancer cells and less toxic to non-malignant human HEK293 and MRC-5 cells at effective concentrations. Overall, according to the MTT, growth kinetics, clonogenic and ROS measurement assay results, we can conclude that NSts treatment has a significant inhibitory effect on glioma cell growth and also created oxidative stress in the cells which is confirmed by using ROS inhibitors NAC. Production of reactive oxygen species (ROS) is initiated after treatment with NSts, followed by apoptotic cell death, which is confirmed by Caspase-3/7 assay. Based on resulting experimental data's, apoptosis occurred in the cells treated with NSts as the level of these executioner caspases was increased many folds over the basal level of untreated cells. This could be due to ROS in cells, which has been shown to be able to induce cell death in several human cancer cells. Decreased viability of glioma cells strongly suggests that oxidative stress induced by NSts resulted in the observed cell death or apoptosis. The importance of such work lies in the possibility that the next-generation NSts might be more efficacious in context of anticancer nano-agents. However, further investigations relating to the size, structure and activity of the NSts as well as their stability under physiological conditions such biological fluids will be helpful and may result in the design of more potent anticancer nano-agents for therapeutic use. Specially, we found the exact concentration of NSts especially NRs and their quantity quantified by statistical analytical parameters for the maximum control of cancer cells. Further studies are in due course to understand, why NSts are responsible for cancer cell death. was dipped in a suspension solution of NSts and dried at room temperature and crystalline, structural morphology property were analyzed at 200 kV.

Human cell culture maintenance and nanostructures treatment. Human cancer cell lines T98G
(glioblastoma), H460 (lung carcinoma), SNU-80 (thyroid cancer) HEK293 (normal embryonic kidney) and MRC5 (normal lung fibroblast) were obtained from KCLB (Korean Cell Line Bank) and cultured in DMEM media (Hyclone, USA) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin solution (Sigma-Aldrich). All cultures were maintained at 37 °C, 95% relative humidity and 5% CO 2 . Cells were seeded in 96-well plates at a concentration of 1 × 10 4 cells/well in 200 μ L of complete media and incubated for 24 hour (hr) at 37 °C in 5% CO 2 atmosphere to allow for cell adhesion. Stock solutions (3 mg/mL) of NSts diluted in PBS and were filter-sterilized, and then further diluted upto 2.92 μ g/mL in incomplete media for treatment against all five malignant and non-malignant cell lines. A 100 μ L solution of NSts (made in cell growth media) was added to a 100 μ L solution of fresh medium in wells to give final concentrations of 1500-2.92 μ g/mL. Control group containing no NSts material was run in triplicates during each assay.
Cell proliferation assays. All malignant and non-malignant cell lines, which were treated with various NSts were used for the MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay to check cytotoxicity. After desired time points, 20 μ L MTT solutions (5 mg/mL) were added to each well and plates were incubated for 3 hr. Following incubation, the medium was removed and the purple formazan precipitate in each well was sterilized in 100 μ L DMSO. Absorbance was measured using Biotek Synergy HT microplate reader at 540 nm and percentage (%) viability was calculated as described previously 51,52 Results are expressed as inhibitory concentration 50 (IC 50 ) were obtained by fitting values to TREND dose− response curves using the routines provided by MicroSoft Excel.
For growth kinetics assay, T98G cells were seeded at 10 4 cells in the 100 mm cell culture plate and their proliferation kinetics were studied at different incubation times (24, 48 and 72 hr) after NSts treatment. Following trypsinization, total cells were counted per plate microscopically using a trypan blue (0.4%) and hemocytometer.
In addition, we have tested the colony formation ability in NSts treated T98G cells. This assay is widely accepted to determine cell reproductive death after treatment with ionizing radiation, but we can also be used to determine the effectiveness of drug molecules. For the clonogenic assay, T98G cells having NSts treatment with the indicated concentrations and plated in the 60 mm cell culture dish after 24 hr. After 8-10 days, the colonies were fixed with methanol and stained with crystal violet. Colonies were quantified using cell counting and the surviving fraction (SF) is calculated. All assays were performed in three independent sets of duplicate tests.
Detection of intracellular ROS. ROS levels were determined by incubating the cells with 10μ M dichlorodihydrofluorescein diacetate (DCFH-DA, Molecular probes, USA) for 30 min at 37 °C. After getting into the cell and cleaved by intracellular esterase, this compound resulting in the formation of fluorescent compound 2' , 7'-dichlorodihydrofluorescin (DCFH). Stained cells were washed twice in PBS, trypsinized and immediately measured fluorescence using plate reader (synergy HT, BioTek) with excitation at 485 nm and emission at 528 nm. The data was analyzed from four individual wells in three independent plates and normalized to control wells.
Executioner caspases detection. Following NSts treatments, T98G cells were subjected to Caspase-3/7 activity measurement with Caspase-Glo 3/7 assay kit (Promega, Madison USA) after 24 hr of incubation. Briefly, 100 μ L of Caspase-Glo reagent was added to each well, the content of well was gently mixed with a plate shaker at 300-500 rpm for 30 seconds (sec). The plate was then incubated at room temperature for 2 hr. The luminescence of each sample wells was measured by the plate luminometer (Synergy HT, BioTek).
Cytogenetic damage analysis. Micronuclei were analyzed in nanostructures treated T98G cells, previously seeded onto coverslips. Following treatment, 24 hr post treatment, cells were fixed with 4% paraformaldehyde (Sigma-aldrich) and subjected to DAPI staining (5 μ g/mL, Sigma-aldrich). Only bi or tri nucleated cells were recorded, which was confirmed by visualization of the cell cytoplasm with phase-contrast microscopy (Ti-U, Nikon).

In vitro migration and invasion assays. Migration and invasion assays were performed by using
Transwell boyden chamber (Corning) according to the manufacturer's instructions. Pre-coated filters (8-μ m pore size, 10 mg/mL growth factor-reduced matrigel) were rehydrated with 100 μ L medium. Then, 2 × 10 4 T98G cells in 200 μ L media were placed in the upper part of each chamber, whereas the lower chamber were filled with 800 μ L cell culture media containing 10% FBS. All procedures were determined by a previously described protocol 53 . Cells were photographed by phase contrast microscopy (Ti-U, Nikon). For migration assay, we used the Transwell chambers with inserts that contained the same type of membrane without the matrigel coating.
RNA extraction and real time PCR. Cells total RNA was isolated using the Trizol reagent (Invitrogen) and cDNA was synthesized using Superscript II reverse transcriptase kit (Invitrogen). To detect the apoptosis-related (Cas3, Cas9, p53, PARP and CYTS) and EMT (N-cadherin and Zeb-1) markers gene expression, significant changes in the mRNA expression were calculated by means of real-time RT-PCR.
Statistical analysis. Data is expressed as a mean ± SD. Statistical analysis was performed by non-parametric Student t-tests. Results were considered significant when p < 0.05.

Statistical determination of NSts suspended in malignant and non-malignant cells.
For the determination and analytical validation of the ZnO nanostructures against cancer and normal cells, cell suspension were further diluted (10 times) and analyzed in terms of analytical parameters such as linearity, range, accuracy, precision and limits of detection (LOD) and limit of quantitation (LOQ) were analyzed. The nanostructures statistical performance were also estimated in terms of mean standard deviation & variance, relative standard deviation, coefficient of correlation, regression line, variance errors in the slope and intercept and confidence limit for the slope and the intercept [39][40][41][42][43] (See supporting information for more detailed description).