Figure 4 | Scientific Reports

Figure 4

From: Identification of two novel Chlorotoxin derivatives CA4 and CTX-23 with chemotherapeutic and anti-angiogenic potential

Figure 4

CTX and its derivatives CA4 and CTX-23 inhibit glioma cell migration.

(A) Representative images of human U251 glioma cell migration was monitored at 0, 12 and 24 hours after scorpion venoms treatment. Low concentration of CTX and its derivatives slowed down the migration and high concentration was able to stop the migration of U251. Scale bar represents 200 μm. (B) Quantification of glioma migration after scorpion venom application (CTX, CA4 and CTX-23) at 0 h, 12 h and 24 h. Quantitative values are given as delta distances from the initial distance at 0 h. (C) Migration assay of rodent F98 glioma cells. Quantification of glioma migration after scorpion venom application (CTX, CA4 and CTX-23) at 0 h, 12 h and 24 h. Quantitative values are given as delta distances from the initial distance at 0 hr. (D) Representative images of three dimensional spheroid migration assay in human U251 glioma cells, cell migration was monitored at 0 and 48 hours after treatment. Scale bar represents 500 μm. (E) Quantification of glioma migration area after scorpion venom application (CTX, CA4 and CTX-23) at 0 h and 48h. Quantitative values are given as delta area from the initial spheroid area at 0 hr. Means are given as ± SD, n = 27 per group, *P < 0.05 (Student’s unpaired t-test).

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