Mesenteric IL-10-producing CD5+ regulatory B cells suppress cow’s milk casein-induced allergic responses in mice

Kim, A-Ram; Kim, Hyuk Soon; Kim, Do Kyun; Nam, Seung Taek; Kim, Hyun Woo; Park, Young Hwan; Lee, Dajeong; Lee, Min Bum; Lee, Jun Ho; Kim, Bokyung; Beaven, Michael A.; Kim, Hyung Sik; Kim, Young Mi; Choi, Wahn Soo

doi:10.1038/srep19685

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Published: 20 January 2016

Mesenteric IL-10-producing CD5⁺ regulatory B cells suppress cow’s milk casein-induced allergic responses in mice

A-Ram Kim¹^na1,
Hyuk Soon Kim¹^na1,
Do Kyun Kim¹^na1,
Seung Taek Nam¹^na1,
Hyun Woo Kim¹^na1,
Young Hwan Park¹^na1,
Dajeong Lee¹^na1,
Min Bum Lee¹^na1,
Jun Ho Lee¹^na1,
Bokyung Kim¹^na1,
Michael A. Beaven²^na1,
Hyung Sik Kim³^na1,
Young Mi Kim⁴^na1 &
…
Wahn Soo Choi¹^na1

Scientific Reports volume 6, Article number: 19685 (2016) Cite this article

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Abstract

Food allergy is a hypersensitive immune reaction to food proteins. We have previously demonstrated the presence of IL-10-producing CD5⁺ B cells and suggested their potential role in regulating cow’s milk casein allergy in humans and IgE-mediated anaphylaxis in mice. In this study, we determined whether IL-10-producing CD5⁺ regulatory B cells control casein-induced food allergic responses in mice and, if so, the underlying mechanisms. The induction of oral tolerance (OT) by casein suppressed casein-induced allergic responses including the decrease of body temperature, symptom score, diarrhea, recruitment of mast cells and eosinophils into jejunum and other biological parameters in mice. Notably, the population of IL-10-producing CD5⁺ B cells was increased in mesenteric lymph node (MLN), but not in spleen or peritoneal cavity (PeC) in OT mice. The adoptive transfer of CD5⁺ B cells from MLN, but not those from spleen and PeC, suppressed the casein-induced allergic responses in an allergen-specific and IL-10-dependent manner. The inhibitory effect of IL-10-producing CD5⁺ B cells on casein-induced allergic response was dependent on Foxp3⁺ regulatory T cells. Taken together, mesenteric IL-10-producing regulatory B cells control food allergy via Foxp3⁺ regulatory T cells and could potentially act as a therapeutic regulator for food allergy.

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Introduction

The prevalence of food allergy, an adverse immune reaction to allergenic food proteins^1,2, is increasing and now affects approximately 6–8% of children in the United States of America. Peanut, milk, egg and shellfish are well recognized as allergens that are responsible for allergic symptoms in patients with diseases such as gastrointestinal food allergy, atopic dermatitis and anaphylaxis^3,4. Among them, cow’s milk allergy (CMA) accounts for 2.5–5% of all allergic diseases and is the one most commonly associated with anaphylaxis and fatalities^5,6,7,8. Cow’s milk protein consists of approximately 80% casein and 20% whey proteins and the major allergenic proteins have been identified within these two groups of proteins^9,10.

The food allergic reactions have been classified under three types, “IgE-mediated” (type I reaction), “non-IgE-mediated (i.e. cell-mediated)” and “combined IgE- and cell-mediated” types¹¹. While the most common form of food allergy is IgE-mediated¹², other immunoglobulins (Ig) such as IgG1 have been implicated in non-IgE-mediated and the mixed IgE/cell-mediated forms of food allergy¹³. Gastrointestinal food allergy belongs to the mixed type and the majority of children with CMA have gastrointestinal symptoms¹⁴.

The various therapies proposed include use of antihistamines, corticosteroids, antagonists against leukotrienes and humanized anti-IgE antibody¹⁵. These therapies however are palliative rather than curative. Allergen-specific immunotherapy (AIT), also called hyposensitization, with incremental increases in dose of allergen was designed to induce specific allergy tolerance in patients with the aim of curing allergic disease instead of alleviating symptoms. Recent publications suggest that AIT is associated with recruitment of Foxp3⁺ regulatory T cells and IL-10-producing B cells, suppression of IgE, induction of IgG4 and suppression of eosinophil and mast cell activity in allergic tissues¹⁶. However, the mechanisms underlying these AIT related events have not been clarified.

Active B cells (B2 cells) positively regulate adaptive immune responses by producing antibody (Ab) and act as antigen-presenting cells to help induce optimal antigen-specific CD4⁺ T-cell activation^17,18,19. However, over the past 30 years, the negative role of unique B cell subsets has also been recognized in mouse autoimmunity and allergic-inflammation models^20,21. Further studies indicate that a specific B subsets including CD5⁺, CD1d^hiCD5⁺ and T2-MZP inhibit immune responses through the production of IL-10 and thus named regulatory B (Breg) cells or B10 cells^22,23. These cells are reported to suppress mouse autoimmunity and allergic inflammation in disease models that include contact hypersensitivity (CHS), experimental autoimmune encephalomyelitis (EAE) and systemic lupus erythematosus (SLE)^18,24,25. We previously described the potential inhibitory role of IL-10-producing CD5⁺ B cells in human food allergic patients^26,27 and in IgE-mediated allergic responses²⁸. However, it is still unclear whether or not IL-10-producing CD5⁺ B cells suppress food allergic responses and, if so, by what mechanism.

In this study, we report that MLN (mesenteric lymph node)-derived IL-10-producing CD5⁺ B cells can suppress casein-induced allergy in a mouse model. The results demonstrate for the first time that this subset of CD5⁺ B suppresses casein-induced allergic responses via induction of Foxp3⁺ regulatory T cells in an IL-10-dependent manner.

Results

The population of IL-10⁺CD5⁺ B cells is increased in casein-induced oral tolerant mice

Regulatory T (Treg) cells are reported to participate in the induction of oral tolerance (OT) in a murine model^6,29,30, but whether regulatory B (Breg) cells play an additional complementary role is unknown. We have investigated this possibility in a casein-induced allergy (CIA) model in mice. The population and frequency of IL-10-producing CD5⁺ B cells and Foxp3⁺ Treg cells increased in MLN of OT mice (as per Fig. 1A) when compared to the PBS-treated normal mice (Fig. 1B), but no significant difference was observed in spleen or other tissues (data not shown). Comparison of various allergic symptoms during CIA (Fig. 1C) revealed that the decline in rectal temperature, allergic symptom score and onset of diarrhea were substantially suppressed in the OT mice compared to non-tolerant mice (Fig. 1D,E). Other relevant events including degranulation of mast cells (Fig. 1F) and infiltration of eosinophils (Fig. 1G) into jejunum after induction of CIA were significantly inhibited in OT mice as well. Furthermore, expression of a representative Th2-related cytokine, IL-4 mRNA, was largely suppressed in the jejunum of OT mice whereas expression of the Th1-related cytokine, IFN-γ, was minimally effected (Fig. 1H). Serum histamine and casein-specific antibodies (IgE, IgG1, IgG2a and IgA) were also much reduced in OT mice (Fig. 1F,I). These data suggest the possible involvement of IL-10⁺CD5⁺ B cells in addition to Foxp3⁺ Treg cells in the induction of OT and the suppression of food allergy in mice.

The population of IL-10⁺CD5⁺ B cells is increased in mesenteric lymph node, but not in spleen and peritoneal cavity, by casein challenge

On investigating the changes in population of all B cells and CD5⁺ B cells specifically in various locations, we observed that the numbers of both populations were increased by CIA in MLN, but not in spleen and peritoneal cavity (PeC) (Fig. 2A). In addition, the population and percentage of IL-10-producing CD5⁺ B cells were significantly elevated by CIA and more so in OT mice (Fig. 2B), suggesting again that IL-10-producing CD5⁺ B cells in MLN are possibly associated with the regulation of food allergy in mice.

The adoptive transfer of CD5⁺ B cells from MLN, but not from spleen and peritoneal cavity, inhibits casein-induced allergic responses

CD5⁺ and CD5⁻ B cells derived from donor mice with OT to casein were transferred into naïve recipient mice which were then subjected to CIA (Fig. 3A). The adoptive transfer of CD5⁺ B cells from MLN, but not those from PeC and spleen, of OT mice suppressed the decrease of rectal temperature, symptom scores (Fig. 3B,C), the expression of Th2 cytokine IL-4 in jejunum and partially reversed the reduction of Th1 cytokine IFN-γ, but not to a significant extent (Fig. 3D), following induction of CIA in the recipient mice. The adoptive transfer of MLN CD5⁺ B cells also reduced the CIA-induced increase in levels of serum IgE and IgG1 (Fig. 3E) and resulted in significant elevation in the number and frequency of IL-10-producing CD5⁺ B cells in MLN (Fig. 3F). Of note, the adoptive transfer of CD5^- B cells had no effect in any of the above experiments (Fig. 3B–F). These results demonstrated that MLN CD5⁺, but not CD5⁻, B cells regulate casein-induced food allergic responses in mice.

The suppressive effect of CD5⁺ B cells on CIA responses is casein-specific

We next investigated whether the inhibition of casein-induced allergic responses by the adoptive transfer of MLN CD5⁺ B cells is casein-specific or not. Inhibition was observed only on adoptive transfer of MLN CD5⁺ B cells from casein-sensitized donor mice, but not by transfer of MLN CD5⁺ B cells from PBS-treated or OVA-sensitized donor mice (Fig. 4A,B). This was true for the decrease in rectal temperature and systemic symptom scores (Fig. 4B), serum levels of casein-specific IgE and IgG1 levels (Fig. 4C) and the increase in the population and number of MLN IL-10-producing CD5⁺ B cells in CIA mice (Fig. 4D). The adoptive transfer of MLN CD5⁺ B cells from PBS-treated or OVA-sensitized mice had no significant effect as compared to the adoptive transfer of MLN CD5⁺ B cells from casein-sensitized mice. The results imply that inhibition of CIA occurs only on adoptive transfer of casein-sensitized MLN CD5⁺ B cells in an allergen-specific manner.

IL-10 from MLN CD5⁺ B cells is critical for the suppression of casein-induced allergic responses

The importance of IL-10-producing CD5⁺ B cells in attenuating CIA was evaluated using IL-10 deficient (IL-10^−/−) mice. The decrease in rectal temperatures and systemic symptom scores during CIA were more pronounced in IL-10^−/− mice than in WT mice (Fig. 5A). Of note, while the adoptive transfer of MLN CD5⁺ B cells from casein-sensitized WT mice to IL-10^−/− recipient mice inhibited CIA reactions, those from IL-10^−/− mice did not (Fig. 5B). The apparent requirement for IL-10 producing CD5⁺ B cells was further confirmed in WT mice by the adoptive transfer of WT or IL-10^−/− MLN CD5⁺ B cells where the latter cells failed to prevent the drop in rectal temperature (Fig. 5C) and the increase of casein-specific serum IgE and IgG1 on induction of CIA (Fig. 5D). Additionally, we confirmed that IL-10-producing CD5⁺ B cells were minimal in CD19^−/−mice which, as previously reported³¹, are incapable of generating this subset of B cells (see supplementary Fig. S1A online). The CIA-induced decrease in rectal temperatures and systemic symptom scores were even more severe in CD19^−/−CIA mice than WT CIA mice (see supplementary Fig. S1B online). Collectively, the results strongly suggest that IL-10-producing subset of CD5⁺ B cells were responsible for the suppressive effect of MLN CD5⁺ B cells on CIA responses.

IL-10-producing CD5⁺ B cells suppresses CIA through the induction of FoxP3⁺ regulatory T cells

IL-10-producing regulatory B cells are reported to suppress allergen-induced airway inflammation via the induction of Foxp3⁺ Treg cells in lung³¹. We have investigated whether Foxp3⁺ Treg cells have a similar role in our model and found that the prevalence and number of Foxp3⁺ Treg cells in the MLN of mice with CIA increased after adoptive transfer of casein-sensitized MLN CD5⁺ B cells, but not MLN CD5⁻ B cells, as compared to control mice without adoptive transfer (Fig. 6A). However, no meaningful change was observed in Foxp3⁺ Treg cell population after adoptive transfer of CD5⁺ B cells from the spleen (data not shown), suggesting that a MLN-specific induction of Foxp3⁺ Treg cells after the adoptive transfer of CD5⁺ B cells could be linked to the inhibitory effect of MLN IL-10-producing CD5⁺ B cells.

To verify the relevance of Foxp3⁺ Treg cells to the inhibition of CIA, we depleted Foxp3⁺ CD25⁺ Treg cells by using anti-CD25 mAb (clone PC61) in recipient mice (Fig. 6B). The accumulation of MLN CD4⁺CD25⁺Foxp3⁺ regulatory T cells during CIA was largely prevented by this treatment (Fig. 6C). The systemic symptoms of CIA and decline in rectal temperature were slightly exacerbated, but not significantly, by anti-CD25 mAb treatment as compared to untreated mice (Fig. 6D). However, the suppressive effect of the MLN CD5⁺ B cells on CIA was not apparent in the Foxp3⁺ Treg cell-depleted mice (Fig. 6D), suggesting that Foxp3⁺ Treg cells are essential for the suppression of CIA by IL-10-producing MLN CD5⁺ B cells.

Discussion

The number of hospitalizations for food-related anaphylaxis has increased rapidly over the past decade^32,33 with the respiratory and circulatory systems being primarily affected³⁴. Common allergens include milk, egg, peanut and shellfish proteins³⁵. Among these cow’s milk is a frequent cause of allergy in infants and young children³⁶ with eczema and itching being the most common symptoms^4,5,36,37. However, in all groups of patients with milk allergy, casein is the principal allergen in cow’s milk^36,38.

Therapeutic approaches for food allergy can be classified into allergen-specific immunotherapy and nonspecific therapies. Allergen-specific immunotherapy includes oral, sublingual and epicutaneous immunotherapy to expose patients to increasing doses of allergen. Nonspecific therapy might include anti-histamine drugs, anti-IgE antibodies, Toll-like receptor agonists and probiotics³³. Despite the advantages of allergen-specific immunotherapy, some patients show no improvement or it proves to be inadvisable due to adverse reactions³⁹.

Medawar and Burnet define immune tolerance as “a state of indifference or non-reactivity towards a substance that would normally be expected to excite an immunological response”⁴⁰. Induction of OT is a well-recognized therapeutic procedure to establish peripheral immune tolerance⁴¹. Treg cells appear to perform a key role in OT in animal models^6,29,30,42. During allergen-specific immunotherapy in humans, Treg cells are generated and suppress Th2 cells and other effector cells in an allergen-specific manner³⁹. In mice, depletion of Treg cells before sensitization to food protein augments the elevation of allergen-specific IgE levels and mast cell degranulation to subsequent oral challenge with food protein⁴³. The adoptive transfer of Treg cells isolated from tolerized mice prevented allergic responses in naïve mice^44,45. Other evidence suggests that Treg cells contribute to oral tolerance in the gastrointestinal tract^45,46 and that Treg cells from MLN are largely involved in the induction of oral tolerance^7,47.

The involvement of IL-10-producing B cell subsets in food allergies is unclear although IL-10–producing Breg cells enable suppression of anaphylaxis, EAE and collagen-induced arthritis in animal models^24,28,48. Also, we have suggested that IL-10-producing CD5⁺ B cells could play a critical role in the suppression of allergic responses in patients allergic to cow’s milk^26,27. In this study, we demonstrate that IL-10-producing CD5⁺ B cell may promote the development of oral tolerance to casein from cow’s milk and describe some of the underlying mechanisms involved. We find that the population of IL-10-producing CD5⁺ B cells is increased in MLN, but not in spleen and PeC, of casein-tolerized mice (Fig. 2). This is consistent with previous reports that oral tolerance cannot be induced in mice lacking MLN^49,50 which appears to be the critical lymphoid tissue. Furthermore, the adoptive transfer of mesenteric CD5⁺ B cells, but not CD5⁻ B cells nor CD5⁺ B cells from spleen or PeC, from casein-tolerized mice suppressed CIA symptoms as well as the induction of casein-specific antibodies and IL-4 in naïve mice (Fig. 3C–E). As CD5⁺ B cells, but not CD5⁻ B cells, produce IL-10²⁸ and CD5⁻ or IL-10-deficient CD5⁺ B cells do not suppress CIA (Fig. 5), IL-10 is a likely participant in the regulation of CIA in mice. Notably, the transfer of casein-sensitized MLN CD5⁺ B cells inhibited only casein-induced allergic responses (Fig. 4), indicating that the effect is allergen-specific.

Our studies also provide evidence for the participation of Treg cells. Studies of other mouse models have demonstrated that B cell deficiency delays the appearance of Treg cells and IL-10 in the central nervous system during EAE. Cooperation between Breg and Treg cells is critical for suppression of EAE development and, as they act at different time points during EAE initiation and progression, these cells may have sequential functions⁵¹. Also, IL-10-producing Breg cells induce pulmonary infiltration of Treg cells which, in turn, inhibit allergic airway inflammation in mice^31,52. Treg cells themselves inhibit the activation of effector cells by allergen⁵³, suppress the influx of eosinophils and effector T cells into inflamed tissues⁵⁴ and inhibit the activation of Th2 cells during allergic responses⁴⁶. We find that Foxp3⁺ regulatory T cells are also essential for the suppression of CIA by IL-10-producing CD5⁺ B cells. The adoptive transfer of IL-10-producing CD5⁺ B cells, but not of CD5⁻ B cells, was found to increase the population of Foxp3⁺CD25⁺ Treg cells in MLN (Fig. 6A). Furthermore, the adoptive transfer of CD5⁺ B cells failed to suppress CIA in Foxp3⁺ CD25⁺ T cell-depleted mice (Fig. 6B–D) to further suggest that the suppressive actions of CD5⁺ B are dependent on Foxp3⁺ CD25⁺ T cells. While our studies demonstrate that Breg cell-derived IL-10 and Fox3⁺ Treg cells are required for development of OT, the exact manner of interaction between these two cell types is unknown but most likely involves the paracrine action of IL-10 on Foxp3⁺ CD25⁺ T cells within MLN (as depicted in Fig. 7).

Additional evidence that B cell-derived IL-10 inhibits CIA comes from studies with CD19-deficient (CD19^−/−) mice. CD19 is an essential surface marker protein for modulating B cell responses⁵⁵. Most B cell subsets are generated normally in CD19^−/−mice⁵⁶, but IL10-producing B cell subsets are barely detectable^25,57 (see supplementary Fig. S1A online). CD19^−/−mice have been used to examine the role of IL-10-producing Breg cells in various diseases²⁵ such as contact hypersensitivity and EAE which are exacerbated in CD19^−/−mice by the absence of IL-10-producing B cells^58,59. The more pronounced allergic responses in CD19^−/−CIA mice in our study (see supplementary Fig. S1B online) suggest also that IL-10-producing CD5⁺ B cells are critical for suppressing CIA responses in mice. However, a caveat in extrapolating observations from mouse models to the clinical situation is that the mechanisms of induction of food tolerance in naïve mice may differ from those in patients where tolerance is induced by reversal of an existing sensitivity to a food allergen. In addition, the underlying pathology or genetic predisposition may also affect the course of desensitization.

In conclusion, MLN-IL-10-producing CD5⁺ B cells suppressed CIA responses in an allergen-specific and Foxp3⁺ Treg cell-dependent manner. This interaction was associated with suppression of immune responses such as infiltration of eosinophils, release of Th2 cytokines, production of IgE and IgG1, mast cell degranulation and secretion of histamine and anaphylactic factors (Fig. 7). Overall, our results suggest that IL-10-producing CD5⁺ B cells are associated with development of oral tolerance for foods and may provide new insight on therapeutic approaches for treatment of food allergies.

Methods

Mice

Wild-type (4-5-week-old female mice), IL-10^−/−(Il10^tm1Cgn) mice or CD19^−/−(Cd19^tm1(cre)Cgn) on C57BL/6 background and BALB/c mice were purchased from The Jackson Laboratory (Bar Harbor, ME). Mice were housed in a pathogen-free facility of Konkuk University (Seoul, Korea). All experiments were performed in accordance with the guidelines and protocols approved by the Institutional Animal Care and Use Committee (IACUC) at Konkuk University.

Induction of casein-mediated food allergy in mice

Female mice were sensitized with 1 mg of casein (Sigma-Aldrich, St. Louis, MO) plus 10 μg of cholera toxin (CT, List Biological Laboratories, Inc., Campbell, CA) by oral administration (p.o.) in 200 μl PBS on days 0 and 7. After this, mice were orally boosted four times for another week, with 5 mg of casein plus 10 μg of CT. One week later, allergic responses were induced by 50 mg of casein in PBS p.o. To induce oral tolerance (OT), mice were administered 1 mg casein p.o. every day for 5 days.

Immediately after challenge, the severity of food allergy was assessed by the change in rectal temperature using a digital thermometer TESTO 925 (Testo AG, Germany). Symptom scores were as follows: 0, no symptoms; 1, scratching around nose and head; 2, puffiness around eyes and mouth; 3, wheezing, labored respiration, cyanosis around mouth and tail; 4, no activity after prodding, or tremor and convulsion; 5, death.

Adoptive transfer of CD5⁺ B cells into mice

Donor mice received casein or ovalbumin at a dose of 1 mg/mouse or PBS daily for 5 days. Lymphocytes were isolated from spleen PeC and MLN from donor mice from which B cells were derived by presorting with CD19 microbeads (Miltenyi Biotec). CD5⁺ or CD5⁻ B cells were subsequently sorted by FACSAria (BD Biosciences, Franklin Lakes, NJ, USA). For adoptive transfer, the isolated CD5⁺ or CD5⁻ B cells (2 × 10⁶cells) were intravenously injected into recipient mice 1 day before the induction of casein-induced food allergy (CIA).

In vivo CD25⁺ T cell depletion

Mice were intraperitoneally injected with 200 μg of anti-mouse CD25 mAb PC61 (eBioscience, San Diego, CA) at days -1 and 13 according to previous reports^7,60. Cell depletion was confirmed by flow cytometry analysis.

Flow cytometry analysis

Single-cell suspensions were isolated from spleen, PeC and MLN tissues. For detection of intracellular IL-10 in B cells, isolated cells were resuspended and incubated for 5h with LPS (10 μg/ml, Sigma), phorbol 12-myristate 13-acetate (PMA, 50 ng/ml, Sigma), ionomycin (500 ng/ml, Sigma) and brefeldin A (3 μg/ml, eBioscience). To detect intracellular Foxp3 in T cells, the isolated cells were resuspended and exposed to the same agents, except LPS, for 4 h. Before staining for cell surface makers, cells were blocked with anti-CD16/CD32 Fc-block (2.4G2, BD Biosciences). Cells were fixed and permeabilized with Cytofix/Cytoperm kit (BD Biosciences) and then were stained with anti-IL-10 (JES5-16E3, eBioscience) and Foxp3 (FJK-16s, eBioscience). Abs against mouse surface proteins were: CD4 (GK1.5), CD5 (53-7.3), CD19 (eBio1D3), CD25 (PC61.5) and B220 (RA3-6B2), all from eBioscience Inc. (San Diego, CA). Cells were analyzed with FACScalibur (Becton Dickinson, San Jose, CA) and FlowJo version 10 software (TreeStar).

Measurement of casein-specific antibody in serum

Serum levels of casein-specific IgA, IgG1, IgG2a and IgE were analyzed using ELISA. A microtiter plate was coated with 50 μg casein/well in buffer (0.1 M sodium carbonate, pH 9.5) and incubated for 18 h at 4 °C. After washing, plates were reincubated for 1h with a blocking PBS buffer, pH 7.0, containing 10% FBS. Serum samples were added to the wells and incubated for 2 h at room temperature (RT). Plates were washed three times with blocking buffer, incubated with 2 μg biotin-labeled rat anti-mouse IgE, IgG1, IgG2a or IgA (BD Biosciences, San Jose, CA) for 1 h at RT and then, after removal of supernatants, washed three times with the blocking buffer. Plates were further incubated for 30 min at RT after addition of a solution (100 μl/well) of streptavidin-HRP (BD Biosciences, San Jose, CA). This solution was replaced with a substrate solution (TMB; GenDEPOT, Barker, TX, USA) (100 μl/well). Absorbance was measured at 450 nm.

Reverse transcriptase-polymerase chain reaction (RT-PCR)

Total RNA was isolated from jejunum by using Trizol reagent (Invitrogen, Carlsbad, CA, USA) and reverse transcribed by use of SuperScript first-strand synthesis system (Invitrogen, Carlsbad, CA). Primers used: mouse IFN-γ (forward, 5΄-AGGAAGCGGAAAAGGAGTCG-3΄; reverse, 5΄-GGGTCACTGCAGCTCTGAAT-3΄); IL-4 (forward, 5΄-TCACTGACGGCAC AGAGCTA-3΄; reverse, 5΄-TGCAGCTCCATGAG AACACT-3΄).

Histological analysis of mast cells and eosinophils

After induction of CIA, isolated jejunums was fixed in 4% paraformaldehyde in PBS, dehydrated by incremental concentrations of ethanol (70 to 100%), washed twice with xylene for 3 min and then embedded in paraffin. Paraffin sections, 5-μm thick, were stained with toluidine blue (for mast cells) or hematoxylin and eosin (for eosinophils). Mast cell and eosinophil numbers in a jejunum were evaluated in 10 sections from five mice. The representative microscopic images were obtained (Olympus DP 70, Tokyo, Japan) at ×400 magnification, HPF.

Histamine assay

Serum was collected within 30 min after oral administration of casein and assayed for histamine by an ELISA kit according to the company’s instructions (Beckman Coulter, Brea, CA).

Statistical analysis

The results were presented as means ± SEM (n ≥ 5). Statistical analysis was determined using the ANOVA followed by Duncan’s test or Dunn’s multiple comparisons test for multiple experimental groups. The symptom score was determined using the Wilcoxon Rank Sum test. Data was analyzed by SigmaStat software (Systat Software, Inc, Point Richmond, CA). In all comparisons, *p < 0.05 and **P < 0.01 were used to determine statistical significance.

Additional Information

How to cite this article: Kim, A.-R. et al. Mesenteric IL-10-producing CD5⁺ regulatory B cells suppress cow's milk casein-induced allergic responses in mice. Sci. Rep. 6, 19685; doi: 10.1038/srep19685 (2016).

References

Wang, J. & Sampson, H. A. Food allergy. J Clin Invest. 121, 827–835 (2011).
CAS PubMed PubMed Central Google Scholar
Reber, L. L. et al. Selective ablation of mast cells or basophils reduces peanut-induced anaphylaxis in mice. J Allergy Clin Immunol. 132, 881–888 (2013).
CAS PubMed PubMed Central Google Scholar
Sicherer, S. H. et al. Immunologic features of infants with milk or egg allergy enrolled in an observational study (Consortium of Food Allergy Research) of food allergy. J Allergy Clin Immunol. 125, 1077–1083 (2010).
CAS PubMed PubMed Central Google Scholar
Docena, G. H., Fernandez, R., Chirdo, F. G. & Fossati, C. A. Identification of casein as the major allergenic and antigenic protein of cow’s milk. Allergy 51, 412–416 (1996).
CAS PubMed Google Scholar
Li, X. M., Schofield, B. H., Huang, C. K., Kleiner, G. I. & Sampson, H. A. A murine model of IgE-mediated cow’s milk hypersensitivity. J Allergy Clin Immunol. 103, 206–214 (1999).
CAS PubMed Google Scholar
Shreffler, W. G., Wanich, N., Moloney, M., Nowak-Wegrzyn, A. & Sampson, H. A. Association of allergen-specific regulatory T cells with the onset of clinical tolerance to milk protein. J Allergy Clin Immunol. 123, 43–52 (2009).
CAS PubMed Google Scholar
Schouten, B. et al. A potential role for CD25⁺ regulatory T-cells in the protection against casein allergy by dietary non-digestible carbohydrates. Br J Nutr. 107, 96–105 (2012).
CAS PubMed Google Scholar
Krishnamurthy, D. et al. Monitoring neutrophils and platelets during casein-induced anaphylaxis in an experimental BALB/c mouse model. Clin Exp Allergy 42, 1119–1128 (2012).
CAS PubMed Google Scholar
van Esch, B. C., Gros-van Hest, M., Westerbeek, H. & Garssen, J. Sensitizing capacity and allergenicity of enzymatically cross-linked sodium caseinate in comparison to sodium caseinate in a mouse model for cow’s milk allergy. Toxicol Lett. 218, 50–55 (2013).
CAS PubMed Google Scholar
Schulmeister, U. et al. Cloning, expression and mapping of allergenic determinants of alphaS1-casein, a major cow’s milk allergen. J Immunol. 182, 7019–7029 (2009).
CAS PubMed Google Scholar
Morita, H., Nomura, I., Matsuda, A., Saito, H. & Matsumoto, K. Gastrointestinal food allergy in infants. Allergol Int. 62, 297–307 (2013).
CAS PubMed Google Scholar
Katz, Y. et al. Early exposure to cow’s milk protein is protective against IgE-mediated cow’s milk protein allergy. J Allergy Clin Immunol. 126, 77–82 (2010).
CAS PubMed Google Scholar
Frossard, C. P., Tropia, L., Hauser, C. & Eigenmann, P. A. Lymphocytes in Peyer patches regulate clinical tolerance in a murine model of food allergy. J Allergy Clin Immunol. 113, 958–964 (2004).
CAS PubMed Google Scholar
Garcia-Careaga, M. Jr. & Kerner, J. A. Jr. Gastrointestinal manifestations of food allergies in pediatric patients. Nutr Clin Pract. 20, 526–535 (2005).
PubMed Google Scholar
Galli, S. J., Tsai, M. & Piliponsky, A. M. The development of allergic inflammation. Nature 454, 445–454 (2008).
ADS CAS PubMed PubMed Central Google Scholar
Akdis, C. A. & Akdis, M. Mechanisms of immune tolerance to allergens: role of IL-10 and Tregs. J Clin Invest. 124, 4678–4680 (2014).
CAS PubMed PubMed Central Google Scholar
Yanaba, K. et al. IL-10-producing regulatory B10 cells inhibit intestinal injury in a mouse model. Am J Pathol. 178, 735–743 (2011).
CAS PubMed PubMed Central Google Scholar
Jin, G. et al. B-cell linker protein expression contributes to controlling allergic and autoimmune diseases by mediating IL-10 production in regulatory B cells. J Allergy Clin Immunol. 131, 1674–1682 (2013).
CAS PubMed Google Scholar
Pieper, K., Grimbacher, B. & Eibel, H. B-cell biology and development. J Allergy Clin Immunol. 131, 959–971 (2013).
CAS PubMed Google Scholar
DiLillo, D. J., Matsushita, T. & Tedder, T. F. B10 cells and regulatory B cells balance immune responses during inflammation, autoimmunity and cancer. Ann N Y Acad Sci. 1183, 38–57 (2010).
ADS CAS PubMed Google Scholar
Kalampokis, I., Yoshizaki, A. & Tedder, T. F. IL-10-producing regulatory B cells (B10 cells) in autoimmune disease. Arthritis Res Ther. 15, S1 (2013).
PubMed PubMed Central Google Scholar
Bouaziz, J. D., Yanaba, K. & Tedder, T. F. Regulatory B cells as inhibitors of immune responses and inflammation. Immunol Rev, 224, 201–214 (2008).
CAS PubMed Google Scholar
Maseda, D. et al. Regulatory B10 cells differentiate into antibody-secreting cells after transient IL-10 production in vivo. J Immunol. 188, 1036–1048 (2012).
CAS PubMed Google Scholar
Matsushita, T., Yanaba, K., Bouaziz, J. D., Fujimoto, M. & Tedder T. F. Regulatory B cells inhibit EAE initiation in mice while other B cells promote disease progression. J Clin Invest. 118, 3420–3430 (2008).
CAS PubMed PubMed Central Google Scholar
Watanabe, R. et al. Regulatory B cells (B10 cells) have a suppressive role in murine lupus: CD19 and B10 cell deficiency exacerbates systemic autoimmunity. J Immunol. 184, 4801–4809 (2010).
CAS PubMed PubMed Central Google Scholar
Noh, J. et al. Characterisation of allergen-specific responses of IL-10-producing regulatory B cells (Br1) in Cow Milk Allergy. Cell Immunol. 264, 143–149 (2010).
CAS PubMed Google Scholar
Noh, J. & Lee, J. H. Regulatory B cells and allergic diseases. Allergy Asthma Immunol Res. 3, 168–177 (2011).
CAS PubMed PubMed Central Google Scholar
Kim, H. S. et al. Interleukin-10-producing CD5⁺ B cells inhibit mast cells during immunoglobulin E-mediated allergic responses. Sci Signal. 8, ra28 (2015).
PubMed Google Scholar
Karlsson, M. R., Rugtveit, J. & Brandtzaeg, P. Allergen-responsive CD4⁺CD25⁺ regulatory T cells in children who have outgrown cow’s milk allergy. J Exp Med. 199, 1679–1688 (2004).
CAS PubMed PubMed Central Google Scholar
Yamashita, H., Takahashi, K., Tanaka, H., Nagai, H. & Inagaki, N. Overcoming food allergy through acquired tolerance conferred by transfer of Treg cells in a murine model. Allergy 67, 201–209 (2012).
CAS PubMed Google Scholar
Amu, S. et al. Regulatory B cells prevent and reverse allergic airway inflammation via Foxp3-positive T regulatory cells in a murine model. J Allergy Clin Immunol. 125, 1114–1124.e8 (2010).
CAS PubMed Google Scholar
Branum, A. M. & Lukacs, S. L. Food allergy among children in the United States. Pediatrics 124, 1549–1555 (2009).
PubMed Google Scholar
Nowak-Węgrzyn, A. & Sampson, H. A. Future therapies for food allergies. J Allergy Clin Immunol. 127, 558–573 (2011).
PubMed PubMed Central Google Scholar
Ahrens, R. et al. Intestinal mast cell levels control severity of oral antigen-induced anaphylaxis in mice. Am J Pathol. 180, 1535–1546 (2012).
CAS PubMed PubMed Central Google Scholar
Sampson, H. A. Update on food allergy. J Allergy Clin Immunol. 113, 805–819 (2004).
CAS PubMed Google Scholar
Ito, K. et al. The usefulness of casein-specific IgE and IgG4 antibodies in cow’s milk allergic children. Clin Mol Allergy 10, 1 (2012).
CAS PubMed PubMed Central Google Scholar
Lee, J. H. et al. Allergen-specific B cell subset responses in cow’s milk allergy of late eczematous reactions in atopic dermatitis. Cell Immunol. 262, 44–51 (2010).
CAS PubMed Google Scholar
Shek, L. P., Bardina, L., Castro, R., Sampson, H. A. & Beyer, K. Humoral and cellular responses to cow milk proteins in patients with milk-induced IgE-mediated and non-IgE-mediated disorders. Allergy 60, 912–919 (2005).
CAS PubMed Google Scholar
Akdis, C. A. Therapies for allergic inflammation: refining strategies to induce tolerance. Nat Med . 18, 736–749 (2012).
CAS PubMed Google Scholar
Sulek, K. Nobel, prize for F. M. Burnett and P. B. Medawar in 1960 for discovery of acquired immunological tolerance. Wiad Lek . 22, 505–506 (1969).
CAS PubMed Google Scholar
Weiner, H. L. Oral tolerance: immune mechanisms and the generation of Th3-type TGF-beta-secreting regulatory cells. Microbes Infect. 3, 947–954 (2001).
CAS PubMed Google Scholar
Schouten, B. et al. Oligosaccharide-induced whey-specific CD25(+) regulatory T-cells are involved in the suppression of cow milk allergy in mice. J Nutr. 140, 835–841 (2010).
CAS PubMed Google Scholar
van Wijk, F. et al. CD4⁺CD25⁺ T cells regulate the intensity of hypersensitivity responses to peanut, but are not decisive in the induction of oral sensitization. Clin Exp Allergy 37, 572–581 (2007).
CAS PubMed Google Scholar
Zhang, X., Izikson, L., Liu, L. & Weiner, H. L. Activation of CD25(+)CD4(+) regulatory T cells by oral antigen administration. J Immunol. 167, 4245–4253 (2001).
CAS PubMed Google Scholar
van Esch, B. C. et al. Oral tolerance induction by partially hydrolyzed whey protein in mice is associated with enhanced numbers of Foxp3⁺ regulatory T-cells in the mesenteric lymph nodes. Pediatr Allergy Immunol. 22, 820–826 (2011).
PubMed Google Scholar
Palomares, O. The Role of Regulatory T Cells in IgE-Mediated Food Allergy. J Investig Allergol Clin Immunol. 23, 371–382 (2013).
CAS PubMed Google Scholar
Tsuji, N. M. & Kosaka, A. Oral tolerance: intestinal homeostasis and antigen-specific regulatory T cells. Trends Immunol. 29, 532–540 (2008).
CAS PubMed Google Scholar
Fillatreau, S., Sweenie, C. H., McGeachy, M. J., Gray, D. & Anderton, S. M. B cells regulate autoimmunity by provision of IL-10. Nat Immunol. 3, 944–950 (2002).
CAS PubMed Google Scholar
Spahn, T. W. et al. Mesenteric lymph nodes are critical for the induction of high-dose oral tolerance in the absence of Peyer’s patches. Eur J Immunol. 32, 1109–1113 (2002).
CAS PubMed Google Scholar
Macpherson, A. J. & Smith, K. Mesenteric lymph nodes at the center of immune anatomy. J Exp Med. 203, 497–500 (2006).
CAS PubMed PubMed Central Google Scholar
Matsushita, T., Horikawa, M., Iwata, Y. & Tedder, T. F. Regulatory B cells (B10 cells) and regulatory T cells have independent roles in controlling experimental autoimmune encephalomyelitis initiation and late-phase immunopathogenesis. J Immunol. 185, 2240–2252 (2010).
CAS PubMed PubMed Central Google Scholar
Natarajan, P. et al. Regulatory B cells from hilar lymph nodes of tolerant mice in a murine model of allergic airway disease are CD5+, express TGF-β and co-localize with CD4+Foxp3+ T cells. Mucosal Immunol . 5, 691–701 (2012).
CAS PubMed PubMed Central Google Scholar
Kashyap, M. et al. Cutting edge: CD4 T cell-mast cell interactions alter IgE receptor expression and signaling. J Immunol. 180, 2039–2043 (2008).
CAS PubMed Google Scholar
Ring, S., Schafer, S., Mahnke, K., Lehr, H. A. & Enk, A. H. CD4⁺ CD25⁺ regulatory T cells suppress contact hypersensitivity reactions by blocking influx of effector T cells into inflamed tissue. Eur J Immunol. 36, 2981–2992 (2006).
CAS PubMed Google Scholar
Fujimoto, M. & Sato, S. B cell signaling and autoimmune diseases: CD19/CD22 loop as a B cell signaling device to regulate the balance of autoimmunity. J Dermatol Sci. 46, 1–9 (2007).
CAS PubMed Google Scholar
Horikawa, M. et al. Regulatory B cell (B10 Cell) expansion during Listeria infection governs innate and cellular immune responses in mice. J Immunol. 190, 1158–1168 (2013).
CAS PubMed Google Scholar
Gà`rdby, E., Chen, X. J. & Lycke, N. Y. Impaired CD40-signalling in CD19-deficient mice selectively affects Th2-dependent isotype switching. Scand J Immunol. 53, 13–23 (2001).
Google Scholar
Matsushita, T. et al. Inhibitory role of CD19 in the progression of experimental autoimmune encephalomyelitis by regulating cytokine response. Am J Pathol. 168, 812–821.
Watanabe, R. et al. CD19 expression in B cells is important for suppression of contact hypersensitivity. Am J Pathol. 171, 560–570 (2007).
CAS PubMed PubMed Central Google Scholar
Boudousquié, C., Pellaton, C., Barbier, N. & Spertini, F. CD4⁺CD25⁺ T cell depletion impairs tolerance induction in a murine model of asthma. Clin Exp Allergy 39, 1415–1426 (2009).
PubMed Google Scholar

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grant (MSIP, No. 2012R1A2A1A03670516) and in part by a National Research Foundation of Korea (NRF) grant (MSIP, NRF-2013R1A4A1069575) funded by the Korea government. Dr. Michael A. Beaven was supported by the Intramural Program of the National Heart, Lung and Blood Institute, NIH.

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Kim A-Ram and Kim Hyuk Soon contributed equally to this work.

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School of Medicine, Konkuk University, 380-701, Chungju, Korea
A-Ram Kim, Hyuk Soon Kim, Do Kyun Kim, Seung Taek Nam, Hyun Woo Kim, Young Hwan Park, Dajeong Lee, Min Bum Lee, Jun Ho Lee, Bokyung Kim & Wahn Soo Choi
Laboratory of Molecular Immunology, National Heart, Lung and Blood Institute, National Institutes of Health, MD20892, Bethesda
Michael A. Beaven
School of Pharmacy, Sungkyunkwan University, 440-746, Suwon, Korea
Hyung Sik Kim
College of Pharmacy, Duksung Women’s University, 132-714, Seoul, Korea
Young Mi Kim

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Contributions

W.S.C. and Y.M.K. provided the idea for this study, interpreted the data and wrote the manuscript; A.R.K. and Hyuk S.K. established food animal model and carried out essential experiments; D.K.K. and D.J.L. performed flow cytometry and collected data; S.T.N., M.B.L. and H.W.K. supported murine food allergy experiments and acquired clinical severity data; Y.H.P. and J.H.L. performed experiments for regulatory T cells; B.K. and Hyung S.K. contributed reagents and knockout animals, designed the experiments and performed statistical analysis; and Y.M.K. and M.A.B. advised and wrote the manuscript.

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Kim, AR., Kim, H., Kim, D. et al. Mesenteric IL-10-producing CD5⁺ regulatory B cells suppress cow’s milk casein-induced allergic responses in mice. Sci Rep 6, 19685 (2016). https://doi.org/10.1038/srep19685

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Received: 06 November 2015
Accepted: 15 December 2015
Published: 20 January 2016
DOI: https://doi.org/10.1038/srep19685

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