Figure 3 : Relationship between different TYLCV DNA amounts and TYLCV susceptibility.

From: Tomato yellow leaf curl virus (TYLCV-IL): a seed-transmissible geminivirus in tomatoes

Figure 3

(A) Symptom development in TYLCV-susceptible Seogwang cultivar tomato showing typical symptoms such as yellowing and curling of leaves and severe stunting (left) and TYLCV-tolerant Bacchus tomato showing no symptoms (right). (B) Identification of Ty-1, 2, and 3 loci. Two different bands were shown in Bacchus cultivar tomato (left). The Ty-2 locus was not confirmed (middle) and the Ty-3a/ty-3 loci was identified from the Bacchus cultivar tomato (right). Lane M, iVDye 100-bp DNA ladder (GenDEPOT, Barker, TX, USA); lane S, TYLCV-susceptible Seogwang cultivar tomato; lane B, TYLCV-tolerant Bacchus cultivar tomato; and lane N, no template control. (C) PCR analysis with leaf and seed samples from TYLCV-inoculated TYLCV-susceptible and -tolerant tomato plants. Lane N, no template control; lane P, positive control with TYLCV-infected tomato genomic DNA; lane L, leaf; lane S1-5, genomic DNA form leaves or seeds of TYLCV-susceptible tomato plants; and lane T1-5, genomic DNA from leaves or seeds of TYLCV-tolerant tomato plants. (D) Relative DNA amounts of TYLCV in TYLCV-susceptible and -tolerant tomato plants germinated from seeds collected from TYLCV-infected tomatoes. Five individual samples for each cultivar were used for analysis. Relative TYLCV DNA amounts were normalized against T1 tomato. Data analyses were conducted using the 2−ΔΔCt method.