Figure 2 : PCR analysis for TYLCV from reproductive tissues and seeds of whitefly-mediated infected and agro-inoculated tomato plants.

From: Tomato yellow leaf curl virus (TYLCV-IL): a seed-transmissible geminivirus in tomatoes

Figure 2

(A) Schematic diagram of preparation of TYLCV-infected tomato plants by whitefly-mediated (WMT) and agro-inoculation (AI) methods. Fruits were harvested from each prepared tomato plant and seeds were collected from tomato fruits. (B) Tomato embryos of seeds collected from tomato fruits and cotyledons and true leaves from seeds. (C) PCR analysis with symptomatic leaves, floral tissues (petal, stamen and pistil), fruits, whole seeds, sterilized seeds, endosperm and embryos of TYLCV-infected tomato plants and cotyledons and the true leaves of their offspring. Lane N, no template control; and lane P, positive control with TYLCV-infected tomato genomic DNA. (D) PCR analysis with leaf, stem and root samples from tomato plants germinated from seeds which were collected from TYLCV-infected tomatoes. Lane N, no template control; lane P, positive control with TYLCV-infected tomato genomic DNA; lane L, leaf; lane S, stem; and lane R, root samples from tomato plants germinated from TYLCV-infected seeds.