Abstract
Resolvins of the D-series are specialized pro-resolving lipid mediators that regulate cellular response by orchestrating resolution networks involved in host responses to injury and infection. Here, endogenous resolvin D4 was identified in human tissues and found to persist late into the resolution phase of acute murine Staphylococcus aureus infections. Completion of the first total synthesis of resolvin D4 established the absolute stereochemical configuration of RvD4 confirmed by matching with endogenous RvD4 from resolving exudates in dorsal pouch S. aureus infections. In vivo, RvD4 (ng/mouse) reduced neutrophilic infiltration (~40%) and enhanced uptake of apoptotic PMN (51%) by human dermal fibroblasts at concentrations as low as 0.1 nM. These results establish the complete stereochemistry of RvD4 as 4S,5R,17S-trihydroxydocosa-6E,8E,10Z,13Z,15E,19Z-hexaenoic acid and its novel pro-resolving actions in S. aureus infections as well as its potent ability to stimulate clearance of apoptotic cells by skin fibroblasts.
Similar content being viewed by others
Introduction
Resolution of acute inflammation involves local-acting chemical mediators that promote cellular trafficking and regulate their function1. Lipid mediators play key roles in regulating cytokine and chemokine production orchestrating the host response to injury and infection2,3. The resolution phase lipid mediators, in addition to regulating leukocytic infiltration, also promote clearance of apoptotic cells, debris and bacteria (reviewed in ref. 1). Chronic inflammation is now thought to arise from a potential failure to resolve local inflammation and is linked with several conditions including rheumatoid arthritis, cardiovascular disease and infection1,4. Resolution mediator networks actively shut down these pathways and regulate pro-resolving pathways that may in theory allow for disease prevention via resolution pharmacology and therapeutic strategies.
Docosahexaenoic acid (DHA) is enriched in the brain, skin, central nervous system and eye. Appropriate consumption of DHA is linked with several wide-ranging effects on health and disease5. Towards appreciating the mechanisms underlying DHA impact, a family of DHA-derived local mediators termed D-series resolvins with unique biological activity and temporal profiles were identified during the resolution phase of acute inflammation. This family of autacoids is positioned at the nexus between a host’s early response to inflammatory challenge and resolution pathways where the beginning or initiating programs signal the end or termination1. Establishing the complete stereochemistry of each specific SPM has permitted confirmation of their potent actions as well as determination of their novel specific biological actions including organ protection, tissue remodeling and microbial containment1,6. From the resolvin (resolution phase interaction products) D series, the absolute stereochemistries of RvD1 (7S,8R,17S-trihydroxydocosa-4Z,9E,11E,13Z,15E,19Z-hexaenoic acid), RvD2 (7S,16R,17S-trihydroxydocosa-4Z,8E,10Z,12E,14E,19Z-hexaenoic acid) and recently RvD3 (4S,11R,17S-trihydroxydocosa-5Z,7E,9E,13Z,15E,19Z-hexaenoic acid), were each established along with select potent biological actions (for example, see ref. 7). As originally described as the biological isolate, RvD4 possesses potent biological actions that include reducing neutrophilic infiltration, inhibiting glial cell cytokine production and regulating leukocyte diapedesis at concentrations as low as 1 nM8. Biologically isolated RvD4 also potently reduces recruitment of neutrophils in murine peritonitis and dorsal skin pouch in response to either opsonized zymosan or TNF-α9. In addition, RvD4 is also produced during ischemic kidney injury and exerts organ protection10.
Given the potent biological actions described for endogenous RvD4, the unambiguous stereochemistry and complete structure of the bioactive RvD4 remained to be established8. The structure of RvD4 was originally deduced based on biosynthetic evidence as well as liquid chromatography-tandem mass spectrometry (LC-MS-MS) and GC-MS metabololipidomic profiling of the bioactive compound from exudates and human leukocytes, which did not allow for establishing the double-bond triene geometry as well as the chirality of the alcohol at the 5 position. In the present report using material obtained by a total organic synthesis approach, we establish the complete stereochemistry of RvD4 as 4S,5R,17S-trihydroxydocosa-6E,8E,10Z,13Z,15E,19Z-hexaenoic acid, confirm its potent biological actions and establish its new specific pro-resolving action in dermal inflammation and infection.
Results
RvD4 is produced in bioactive levels during S. aureus infection
To identify relevant SPMs with protective actions during the resolution programme we used a well-established dorsal pouch model for murine S. aureus skin infection6 with focus on the endogenous formation and appearance of Resolvin D4 (Fig. 1). Inoculation of S. aureus at 105 c.f.u. gave a self-limited inflammatory response with polymorphonuclear leukocyte (PMN) numbers reaching a maximum at 12 hr (Tmax), followed by a sharp decline and return to basal levels by 24 hr. At 12 hr, monocytes/macrophages gave a more persistent presence and remained elevated throughout the resolution phase into the later time points (Fig. 2A), consistent with their role in clearance of apoptotic PMN and cellular debris8,11.
In this time course, the LM and SPM profiles from infectious exudates were monitored using LC-MS-MS-based lipid mediator metabololipidomics. The inflammation initiating prostaglandins and leukotrienes LTB4, PGE2 and PGD2 increased sharply in response to a bacterial inoculation followed by a return to basal levels (Fig. 2B,C). In these infectious purulent exudates we normalized the recovery based on individual lavage volume to determine the temporal profile of the D-series resolvins and found them present in low picomolar concentrations. RvD1 and RvD2 reached a maximum presence at 12 hr. RvD3 was observed at low levels throughout the time course of self-limited infection whereas RvD4 gave a unique pattern with its production persisting late into the resolution phase. These results suggest that RvD4 is constituently present and is differentially regulated and produced to regulate processes in the resolution of infection (Fig. 2D,E).
Total organic synthesis of RvD4
To establish the complete stereochemistry for RvD4 and assign the double bond geometry around C7 to C11 and the chirality of the alcohol at the C5 position, we employed a total organic synthesis approach that was also used in establishing the complete stereochemistry for other SPM12. Along these lines the complete total synthesis of RvD4 was accomplished using a 22-step total organic synthetic scheme, to afford stereochemically pure 4S,5R,17S-trihydroxydocosa-6E,8E,10Z,13Z,15E,19Z-hexaenoic acid, RvD4, shown in Fig. 3A. The hydroxyl groups positioned at the 4S, 5R carbon positions proved similarly to be a problematic complication in which the ester precursor rearranged to form a mixture of the ester as well as the 5- and 6-membered lactone, as observed similarly with the synthesis of RvD312.
With these aforementioned challenges in mind we began our synthesis with the ring opening of chirally pure d-erythrose to set the 4S, 5R hydroxyl groups in the proposed stereoconfiguration. Next, we took advantage of a Wittig coupling to form the carbon-carbon bond at the 6-position between the Wittig salt and aldehyde and through additional manipulation built the conjugated triene system. The 17S-hydroxy position was set in place through a ring opening of chiral R-glycidol. The final carbon-carbon bond was formed through a copper-mediated cross coupling of an allylic-bromide with terminal alkyne C11 to afford the protected bis-acetylenic precursor in good yield. Because of the sensitive nature of each metabolite, RvD4 at this stage required careful handling. The bis-acetylenic precursor was reduced prior to the deprotection; however, Zn/Cu/Ag hydrogenation was too mild in the presence of bulky protecting groups. Therefore, Lindlar catalyst was employed and subsequent desilylation allowed for the synthesis of the final compound 4S,5R,17S-trihydroxydocosa-6E,8E,10Z,13Z,15E,19Z-hexaenoic acid without decomposition. The absolute structure was confirmed by 2D 1H-1H nuclear magnetic resonance (NMR) spectroscopy for full proton assignment (Fig. 3B).
Matching of endogenous RvD4 with synthetic materials
We next matched the physical properties of the material obtained by total organic synthesis to that of endogenous RvD4. Our first criterion for the matching of authentic material with synthetic compound was by retention time. The chromatographic signature of both the endogenous and synthetic material demonstrated identical retention profiles by reverse phase high pressure liquid chromatography (HPLC) with a sharp peak eluting with retention time (TR) of 12.2 min (Fig. 4A,B). Coinjection of the authentic RvD4 isolated from purulent inflammatory exudates with synthetic compound confirmed that these products co-eluted (Fig. 4C).
Further validation of synthetic material was confirmed by assessing the UV absorbance profile and tandem mass spectrometry (MS-MS) patterns. The synthetic material displayed UV absorbance profile with a triplet band of absorption, , at 275 nm and shoulders at 266 and 285 that is characteristic of a triene conjugated double-bond system1. The synthetic material also gave a broad shoulder with absorption at ~226 nm (Fig. 4A inset) that is characteristic of a conjugated double-bond system. This absorbance profile was in accord with published values for endogenous RvD48. We next obtained MS-MS fragmentation pattern using an LC-MS-MS metabololipidomics-based approach as in ref. 13, demonstrating that the synthetic and endogenous RvD4 matched in physical properties. The MS-MS profile for endogenous RvD4 as well as its synthetic material gave a parent ion with m/z 375 = M-H and daughter ions with m/z 357 = M-H-H2O, m/z 339 = M-H-2H2O, m/z 331 = M-H-CO2, m/z 313 = M-H-H2O-CO2, m/z 295 = M-H-2H2O-CO2, m/z 259 = 277-H2O, m/z 255 = 273-H2O, m/z 241 = 277 -2H2O, m/z 237 = 273-2H2O, m/z 233 = 277-CO2, m/z 225 = 243-H2O, m/z 215 = 277-H2O-CO2, m/z 113 = 131-H2O (Fig. 4D,E). To place RvD4 within the D-series resolvins we profiled the infectious exudates and found that RvD4 eluted last of the trihydroxyl resolvin family giving it the most lipophilic characteristic of the four (Fig. 4F).
RvD4 stops neutrophil infiltration in sterile peritonitis and S. aureus skin infection
We next sought to confirm the potent anti-inflammatory and pro-resolving actions of RvD4 with synthetic material. First we assessed its actions in regulating the acute inflammatory response initiated by zymosan A in a peritonitis model. Zymosan administration via i.p. injection gave rapid PMN infiltration into the peritoneal cavity at 4 hr. Shown on the representative dot plots and bar graphs, RvD4 at 10 ng/mouse decreased percent of PMN, giving ~31% reduction at 4 hours (Fig. 5A). For comparison, administration of an equal amount of the potent immunoresolvent RvD37 also gave ~44% reduction in PMN infiltration. These results confirm the potent pro-resolving anti-inflammatory properties initially described for the bioactive RvD48 with synthetic material.
With the biological activity of RvD4 confirmed, we next assessed its in vivo actions in S. aureus-mediated infection probing its action as well as temporal formation. To determine its role in bacterial killing and host protection during S. aureus infections, RvD4 was administered at 200 ng/mouse concomitantly with a self-limited bacterial inoculation (105 c.f.u) into the dorsal pouch of male FVB mice. Resolution indices were then measured. RvD4 potently reduced neutrophilic infiltration by ~40% at Tmax (12 h) and shortened the resolution interval in mice treated with RvD4 from 6 to 3 hr (Fig. 5B). Analysis by histochemical staining of dorsal pouch lining from mice administered vehicle or RvD4 gave lower PMN levels in the cavity in mice treated with RvD4 (Fig. 5C). Treatment with RvD4 also significantly lowered bacterial counts when compared to vehicle control (Fig. 5D). In addition, RvD4 enhanced the phagocytosis of S. aureus by isolated mouse macrophages by ~40% at 10nM (data not shown). RvD4 also regulated inflammation-initiating eicosanoids in the inflammatory exudates including LTB4 and PGD2 by 68 and 48% respectively, demonstrating potent anti-inflammatory actions in vivo (Fig. 5E–G).
RvD4 exerts pro-resolving actions with human neutrophils and macrophages
To further elucidate its host-protective actions, we sought to establish the potent anti-inflammatory and pro-resolving actions of RvD4 with isolated human leukocytes. RvD4 (0.01 nM to 10 nM) promoted bacterial phagocytosis by human macrophages (12–18%) to a similar extent as the pro-resolving mediator RvD3 (Fig. 6A). RvD4 also promoted the phagocytosis of opsonized zymosan A at concentrations as low as 0.1–10 nM, significantly enhancing macrophage uptake by 40–60% above vehicle controls (Fig. 6B).
The clearance of apoptotic PMN during inflammation is a cellular hallmark in resolution1,14. To determine RvD4’s role in the clearance of apoptotic neutrophils we assessed whether RvD4 promoted macrophage efferocytosis. RvD4 statistically significantly increased macrophage efferocytosis of apoptotic neutrophils by 40–50% at concentrations as low as 0.1–10 nM (Fig. 6C). These results suggest that RvD4 enhances human macrophage functions in clearing cellular debris and microbial particles. Together these results provide evidence that RvD4 is pro-resolving with human phagocytes.
RvD4 exerts pro-resolving actions with human fibroblasts: Comparison with RvD1 and MaR1
Given that RvD4 displayed potent pro-resolving actions in skin infection, we investigated whether it also acts on human dermal fibroblasts. RvD4 potently promoted the clearance of apoptotic PMN by human fibroblasts (Fig. 6D) by ~59% at 1 nM. Of the SPM tested, RvD4 and RvD1 at 1 nM gave the highest increase in apoptotic cell uptake (~52 and 57%). These findings demonstrate new action of SPM such as RvD1 and MaR1 (7R,14S-dihydroxydocosa-4Z,8E,10E,12Z,16Z,19Z-hexaenoic acid) in resolution and that RvD4 displays potent actions with human dermal fibroblasts (Fig. 6E).
RvD4 identification in tissue in human and marine organisms
Since SPMs are identified in marine organisms and human tissues, suggesting potential physiologic role(s) in these organisms1,15, we next assessed the presence of RvD4 in relation to other members of the D-series resolvins in human plasma, human serum13, human breast milk16 as well as murine spleen, brain and sardines (Table 1). The presence of endogenous RvD4 in these samples at picogram levels (10–100 pg) suggests a utility in the evolutionary conservation in structure function across species associated with its role in the resolution process.
Discussion
In the present report we establish the complete stereochemical assignment of RvD4 as 4S,5R,17S-trihydroxydocosa-6E,8E,10Z,13Z,15E,19Z-hexaenoic acid by matching endogenous material isolated from purulent inflammatory exudates with synthetic compound (Fig. 4) made by total organic synthesis (Fig. 3). RvD4 was temporally regulated during S. aureus infection as well as remained at bioactive levels (~ pM) in the late stages of resolution (Fig. 2). Administration of RvD4 in vivo counter regulated inflammatory initiating eicosanoids in S. aureus infection and shortened resolution indices during infection (Fig. 5), concepts on which the edifice of resolution is based. Administration of RvD4 in both sterile inflammation and microbial infection gave a reduction in neutrophil recruitment in vivo (Figs 5 and 6). RvD4 also enhanced macrophage responses with human macrophages and enhanced fibroblast responses with human fibroblasts in efferocytosis of apoptotic PMN and sterile zymosan (Fig. 6). Furthermore RvD4 was found to be present in a number of tissues across species suggesting its utility through evolutionary conservation (Table 1).
The biosynthesis of this family of compounds by lipoxygenase enzymes affords their unique structures and function. RvD4 and its congener RvD3 are formed via a common 4, 5-epoxide involving a second oxygenation pathway by lipoxygenase enzymes. The divergence between RvD3 and RvD4 occurs from the insertion of water by enzymatic hydrolysis at the C11 and C5 position through a carbocation intermediate to afford RvD3 and RvD4, respectively (Fig. 1). Both compounds have distinct behaviors based on their arrangement of chiral hydroxyl stereocenters and double-bond triene-diene configuration giving them each a distinct activity profile and set of physical characteristics such as absorbance and retention time. Synthetic RvD3 demonstrated greater potency in a mouse peritonitis model (Fig. 5) while synthetic RvD4 significantly increased efferocytosis in human fibroblasts by promoting the clearance of apoptotic PMN, while RvD3 was not significant (Fig. 6).
Resolvins can alleviate both the bacterial burden in a skin S. aureus infection as well as in lung infection models6,17. Resolvins are produced and act locally in a time-dependent fashion to intercede with an overbearing neutrophilic response that can lead to chronic inflammation, tissue fibrosis and scarring. Antibiotic-resistant S. aureus skin infections are widespread and a leading cause of hospital-acquired infection6. The acute inflammatory response involves a number of inflammation-initiating chemical mediators including thromboxanes, prostaglandins and leukotrienes as well as cytokines and chemokines. The D-series resolvins arbitrate this interaction by shutting down proinflammatory pathways and turning on resolution networks8,11,18,19. In the S. aureus infection model, RvD1 and RvD2 levels traced with the eicosanoids followed by a temporally distinct response of Resolvin D3 and D4 at later time points, thus suggesting differential roles in the 7,8 vs. 4,5 epoxide pathway shared by RvD4 and RvD3 during late-stage clearance through phagocytosis of foreign bacteria and apoptotic PMN by macrophages and dermal fibroblasts (Fig. 6). Administration of RvD4 in vivo stopped leukocyte influx to the site of infection in the dorsal pouch cavity as well as the inflammation-initiating eicosanoids by reducing levels of PGD2 and LTB4.
Earlier work has shown that SPM actions are not limited to leukocytes but can stimulate endothelial cell-leukocyte interactions, suggesting a more prominent role in the resolution phase. Fibroblasts are known to be a critical component in wound healing and tissue remodeling for their restorative properties in supporting the structural framework for tissue and repair20,21. Lung fibroblasts and periodontal ligament fibroblasts have been shown to respond to SPMs under smoke-induced lung inflammation22 and periodontal disease23, respectively. Dermal fibroblasts were found to phagocytize apoptotic PMN and opsonized beads as previously reported in refs 24 and 25 and this action was enhanced by RvD4, providing a novel mechanism of action in the resolution of dermal infectious inflammation. The role SPM play in signaling dermal fibroblasts during resolution has not been investigated before. We investigated RvD4’s role in signaling dermal fibroblast clearance of apoptotic PMN and found RvD4 actively promotes phagocytosis at subnanomolar concentrations, suggesting it as a critical player in the resolution regiment of this potent class of autacoids.
In summation, the present findings report the complete stereochemistry of RvD4, endogenous production of RvD4 during infection, RvD4’s regulation of leukocyte recruitment in sterile inflammation and infection, counter-regulation of eicosanoids, phagocyte-directed action with human macrophages (defining its pro-resolving actions), increase of fibroblast phagocytosis as well as RvD4 identification across several species. These findings suggest RvD4 plays a pivotal role(s) in infectious resolution as well as gives insight into its temporal vignette in the endogenous production of these potent SPM-autacoids during resolution and return to homeostasis.
Significance
These results establish RvD4 stereochemistry and role in host protection and resolution of inflammation during bacterial infection on specific target cell types including neutrophils and macrophages. RvD4 diminishes inflammation by promoting clearance of a bacterial burden and promotes the efferocytosis of apoptotic PMN. For the first time an SPM was shown to have a direct proresolving action on a new target cell type, human dermal fibroblasts, where RvD4 potently stimulates phagocytosis and efferocytosis of cellular debris and apoptotic neutrophils. The complete stereochemical assignment of RvD4 with its synthetic precursor was confirmed by matching both physical properties as well as biological actions to confirm the complete stereochemical assignment, which now opens a new path for therapeutic developments based on RvD4 structure and action. The identification of endogenous D-series resolvins and RvD4 displays a unique spatio-temporal ensemble and is produced at biologically relevant levels during S. aureus-initiated infection suggesting an endogenous host defense mechanism triggering resolution and host protection during infectious inflammation. These results from RvD4 provide structural evidence for a new approach to host-directed therapies that now take into account the role of fibroblasts in resolution as well as infection.
Methods
Materials
RPMI 1640 and DPBS (with or without calcium and magnesium) were purchased from Lonza (Hopkinton, MA, USA). Ficoll-Histopaque 1077-1, lipopolysaccharide (LPS) and zymosan A were obtained from Sigma-Aldrich (St. Louis, MO, USA). Human recombinant granulocyte-monocyte colony stimulating factor (GM-CSF), macrophage colony-stimulating factor, interferon γ (IFN-γ) and interleukin-4 were purchased from R&D Systems (Minneapolis, MN, USA). Fetal calf sera, ampicillin and bac-to-bac baculovirus expression system were from Invitrogen (Grand Island, NY, USA). FITC rat anti-mouse Ly6G (clone IAS) and purified rat anti-mouse CD16/32 (mouse BD Fc block) were purchased from BD Biosciences (San Jose, CA) and PE rat anti-mouse F4/80 (clone BM8) and PerCP/Cy5.5 rat anti-mouse CD11b (clone Mac-1) were purchased from eBioscience (San Diego, CA). C18 SPE columns were from Waters (Milford, MA, USA). Poroshell C18 columns were from Phenomenex; fluorescently conjugated zymosan A was from Invitrogen. All liquid chromatography solvents were from Fisher Scientific (Pittsburgh, PA, USA). DHA along with d4-leukotriene B4 (d4-LTB4), d5-lipoxin A4 (d5-LXA4), d4-prostaglandin E2 (d4-PGE2), d8-5S-HETE and d5-Resolvin D2 (d5-RvD2) were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Fresh human leukocytes were prepared from peripheral blood of deidentified and consenting healthy volunteers. Informed consent was obtained from all subjects. The protocol was approved by the Partners Human Research Committee (Protocol number 1999P001297) and was in accordance with the Declaration of Helsinki.
Bacteria growth
S. aureus were cultured in LB broth and harvested at mid-log phase (OD600 ~ 0.5, 5 × 108 c.f.u./ml) and washed in sterile saline before inoculation into mouse dorsal pouch.
NMR of Synthetic RvD4
The proton chemical shift and coupling constants for RvD4 were recorded as follows (see Fig. 2 for further details). 1H NMR (600 MHz, MeOD) δ 6.51–6.43 (dd, J = 15.0, 11.1 Hz, 2H), 6.27 (dd, J = 15.3, 10.7 Hz, 1H), 6.15 (dd, J = 14.7, 10.7 Hz, 1H), 5.94 (t, J = 10.9 Hz, 1H), 5.90 (t, J = 10.9 Hz, 1H), 5.74 (dd, J = 15.2, 6.8 Hz, 1H), 5.59 (dd, J = 15.0, 6.6 Hz, 2H), 5.41–5.34 (m, 1H), 5.33–5.23 (m, 3H), 4.04 (q, J = 6.5 Hz, 1H), 3.89 (t, J = 6.0 Hz, 1H), 3.45–3.39 (m, 1H), 3.00 (t, J = 7.7 Hz, 2H), 2.32–2.13 (m, 4H), 1.95 (q, J = 7.6 Hz, 2H), 1.75 (ddd, J = 14.7, 7.1, 3.2 Hz, 1H), 1.58 (dq, J = 15.1, 7.5 Hz, 1H), 0.86 (t, J = 7.5 Hz, 3H).
Acute Inflammation
S. aureus (105 c.f.u./mouse) microbial infection was initiated in dorsal pouch of mice (6 to 8 weeks-old FVB mice) purchased from Charles River Laboratories and fed Laboratory Rodent Diet 20–5058 (Lab Diet, Purina Mills). Experiments were approved by the Standing Committee on Animals of Harvard Medical School (protocol no. 02570) and complied with institutional and U.S. National Institutes of Health (NIH) guidelines.
Dorsal skin pouch
Mice were anesthetized with isoflurane and 3.0 ml of sterile air was injected subcutaneously on days 0 and 3 between the shoulders and into the back of male mice. On day 6, S. aureus (105 c.f.u./mouse) was administered by intra-pouch injection in combination with either RvD4 (200 ng/mouse) or sterile saline. No apparent changes were observed in animal behavior and mice were euthanized and lavages were collected at designated time points (4, 12, 24, 36, 48 and 72 hr) with 3 mL DPBS−/−. Leukocytes were enumerated and differential counts conducted by flow cytometry and graphed by curve smoothing. Aliquots of lavage were serial diluted and plated on agar plates and cultured overnight at 37 °C to determine bacterial counts. Skin punch biopsies were collected and stained for hematoxylin-eosin staining.
Murine peritonitis
1 mg/ml of zymosan was injected i.p. in parallel with RvD3 (10 ng), RvD4 (10 ng), or vehicle in 100 μl saline. At 4 h, peritoneal lavages were collected and cell counts enumerated by flow cytometry.
Flow Cytometry
Air pouch exudates were lavaged and analyzed following previous procedure outlined in ref. 7. Cells were suspended in FACS buffer (5% BSA in DPBS) and incubated for 40 min at 4 °C with individual antibody or combinations of FITC-conjugated anti-mouse LY6G (clone 1AS) for PMN and PE-conjugated anti-mouse F4/80 Ab (clone BM8) for MΦ or PerCP-conjugated anti-mouse CD11b (clone M1/70) to determine leukocyte sub-types using a FACS Canto II flow cytometer. Antibodies were from BD Biosciences (San Jose, CA) and eBioscience (San Diego, CA).
Leukocyte Functional Responses
Normal – Human Dermal Fibroblast phagocytosis and efferocytosis
Normal - Human Adult Dermal Fibroblasts (NHDF-Ad, Lonza) were thawed and grown in FBM growth medium (0.1% insulin, 0.1% rhFGF-B, 0.1% GA-1000, 2% FBS) for 3 days until 80–100% confluent. Cells were used within four healthy passages for phagocytosis assays. NHDF-Ad were plated into 96-well plates (Costar) at 5 × 103 cells/well and cultured overnight. To assess phagocytosis, NHDF-Ad were pretreated with RvD3, RvD4 or vehicle (0.1–10 nM for 15 min at 37 °C) followed by incubation of fluorescent-labeled S. aureus (50:1) for 60 min. Phagocytosis of fluorescent-labeled S. aureus was assessed using an M3 SpectraMax plate reader at λ exc\λ em - 480\516. Neutrophils for efferocytosis were isolated from human whole blood (obtained from consenting healthy human volunteers under protocol #1999-P001297 approved by Partners Human Research Committee) by density separation using Ficoll-Histopaque 1077-1. Cells were centrifuged (300 g for 30 minutes at 4 °C) and red blood cells were lysed to afford pure PMN. Freshly isolated PMN were stained with fluorescent nuclear dye bisbenzimide (Hoechst 33342; Sigma Aldrich; 1X concentration for 30 minutes at 37 °C) and cultured overnight (3 × 106 cells/well in DPBS+/+). Before assessing efferocytosis, NHDF were pretreated with either RvD4 (0.1–10 nM), RvD1 (1 nM), RvD2 (1 nM), RvD3 (1 nM), MaR1 (1nM) or vehicle for 15 min at 37 °C. Fluorescent-labeled apoptotic PMN (3:1) were incubated for 60 min and efferocytosis was assessed using an M3 SpectraMax plate reader at 346, 460 nm respectively.
Human macrophage phagocytosis and efferocytosis
Human macrophages were prepared from peripheral blood mononuclear cells from the Children’s Hospital Boston Blood Bank and isolated by density-gradient Ficoll-Histopaque isolation. These were differentiated and cultured for 7 days in RPMI 1640 (10% fetal bovine serum) and GM-CSF (20 ng/mL). Macrophages were plated into 96-well plates (Costar) at 5 × 104 cells/well the previous night and were pretreated with either RvD3, RvD4 or vehicle (0.1–10 nM for 15 min at 37 °C) followed by incubation of FITC labeled opsonized zymosan at 37 °C for 60 min or fluorescent-labeled S. aureus (50:1) for 60 min and assessed using an M3 SpectraMax plate reader at λ exc\λ em - 493/525 and 480\516, respectively. Efferocytosis was assessed by pretreating macrophages with RvD3, RvD4 (0.1–10 nM) or vehicle for 15 min at 37 °C. Fluorescent-labeled apoptotic PMN (3:1) were incubated for 60 min and efferocytosis was assessed using an M3 SpectraMax plate reader at λ exc\λ em - 346, 460 nm, respectively.
Targeted Lipid Mediator Profiling
All samples and enzyme incubations were stopped with two volumes methanol and by solid-phase extraction on C18 SPE columns (Waters). 500 pg of a deuterated standard mixture containing, d4-LTB4, d4-LXA4, d4-PGE2, d8-5S-HETE and d5-RvD2 was added to determine recovery and quantification. Mediators were analyzed using LC-MS-MS analysis by a Qtrap 6500 (ABSciex) equipped with an Agilent HP1100 binary pump and diode-array detector on a Agilent Poroshell 120 EC-C18 column (4.6 mm × 100 mm × 2.7 μm) with a gradient of methanol/water/acetic acid of 55:45:0.01 (v/v/v) to 100:0:0.01 at 0.5 mL/min flow rate. To identify and quantitate SPMs, we used multiple reaction monitoring (MRM) at the transition ion pair (375 > 101) with signature ion fragments specific for RvD4 (at least 6 diagnostic ions and calibration curves).
Statistics
Results are expressed as means ± SEM. Comparison between two groups was analyzed using Student’s t test and between multiple groups using ANOVA. Statistical significance was considered to be P < 0.05.
Additional Information
How to cite this article: Winkler, J. W. et al. Resolvin D4 stereoassignment and its novel actions in host protection and bacterial clearance. Sci. Rep. 6, 18972; doi: 10.1038/srep18972 (2016).
References
Serhan, C. N. Pro-resolving lipid mediators are leads for resolution physiology. Nature 510, 92–101 (2014).
Samuelsson, B. Role of basic science in the development of new medicines: Examples from the eicosanoid field. J. Biol. Chem. 287, 10070–10080 (2012).
Haeggstrom, J. Z. & Funk, C. D. Lipoxygenase and leukotriene pathways: Biochemistry, biology and roles in disease. Chem. Rev. 111, 5866–5898 (2011).
Ward, P. A. New approaches to the study of sepsis. EMBO Mol Med 4, 1234–1243 (2012).
De Caterina, R. N-3 fatty acids in cardiovascular disease. N. Engl. J. Med. 364, 2439–2450 (2011).
Chiang, N. et al. Infection regulates pro-resolving mediators that lower antibiotic requirements. Nature 484, 524–528 (2012).
Dalli, J. et al. Resolvin D3 and aspirin-triggered resolvin D3 are potent immunoresolvents. Chem. Biol. 20, 188–201 (2013).
Serhan, C. N. et al. Resolvins: A family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter pro-inflammation signals. J. Exp. Med. 196, 1025–1037 (2002).
Hong, S., Gronert, K., Devchand, P., Moussignac, R.-L. & Serhan, C. N. Novel docosatrienes and 17s-resolvins generated from docosahexaenoic acid in murine brain, human blood and glial cells: Autacoids in anti-inflammation. J. Biol. Chem. 278, 14677–14687 (2003).
Duffield, J. S. et al. Resolvin D series and protectin D1 mitigate acute kidney injury. J. Immunol. 177, 5902–5911 (2006).
Tabas, I. & Glass, C. K. Anti-inflammatory therapy in chronic disease: Challenges and opportunities. Science 339, 166–172 (2013).
Winkler, J. W., Uddin, J., Serhan, C. N. & Petasis, N. A. Stereocontrolled total synthesis of the potent anti-inflammatory and pro-resolving lipid mediator resolvin D3 and its aspirin-triggered 17R-epimer. Org Lett 15, 1424–1427 (2013).
Colas, R. A., Shinohara, M., Dalli, J., Chiang, N. & Serhan, C. N. Identification and signature profiles for pro-resolving and inflammatory lipid mediators in human tissue. Am J Physiol Cell Physiol 307, C39–54 (2014).
Poon, I. K., Lucas, C. D., Rossi, A. G. & Ravichandran, K. S. Apoptotic cell clearance: Basic biology and therapeutic potential. Nat Rev Immunol 14, 166–180 (2014).
Oh, S. F., Vickery, T. W. & Serhan, C. N. Chiral lipidomics of e-series resolvins: Aspirin and the biosynthesis of novel mediators. Biochim. Biophys. Acta. 1811, 737–747 (2011).
Weiss, G. A. et al. High levels of anti-inflammatory and pro-resolving lipid mediators lipoxins and resolvins and declining docosahexaenoic acid levels in human milk during the first month of lactation. Lipids Health Dis 12, 89 (2013).
El Kebir, D., Gjorstrup, P. & Filep, J. G. Resolvin e1 promotes phagocytosis-induced neutrophil apoptosis and accelerates resolution of pulmonary inflammation. Proc. Natl. Acad. Sci. USA 109, 14983–14988 (2012).
Fredman, G. et al. Resolvin D1 limits 5-lipoxygenase nuclear localization and leukotriene b4 synthesis by inhibiting a calcium-activated kinase pathway. Proc. Natl. Acad. Sci. USA 111, 14530–14535 (2014).
Dinarello, C. A., Simon, A. & van der Meer, J. W. Treating inflammation by blocking interleukin-1 in a broad spectrum of diseases. Nat Rev Drug Discov 11, 633–652 (2012).
Edwards, J. C. W., Sedgwick, A. D. & Willoughby, D. A. The formation of a structure with the features of a synovial lining by subcutaneous injection of air: An in vivo tissue culture system. J. Pathol. 134, 147–156 (1981).
Qu, X. et al. Resolvins e1 and D1 inhibit interstitial fibrosis in the obstructed kidney via inhibition of local fibroblast proliferation. J. Pathol. 228, 506–519 (2012).
Hsiao, H. M. et al. A novel anti-inflammatory and pro-resolving role for resolvin D1 in acute cigarette smoke-induced lung inflammation. PLoS One 8, e58258 (2013).
Mustafa, M. et al. Resolvin D1 protects periodontal ligament. Am J Physiol Cell Physiol 305, C673–679 (2013).
Hall, S. E., Savill, J. S., Henson, P. M. & Haslett, C. Apoptotic neutrophils are phagocytosed by fibroblasts with participation of the fibroblast vitronectin receptor and involvement of a mannose/fucose-specific lectin. J. Immunol. 153, 3218–3227 (1994).
Grinnell, F. Fibroblast spreading and phagocytosis: Similar cell responses to different-sized substrata. J. Cell. Physiol. 119, 58–64 (1984).
Acknowledgements
The authors thank Mary Halm Small for expert assistance in manuscript preparation. In addition we thank Dr. Birgitta Schmidt (Boston Children’s Hospital Department of Pathology) for histology and Dr. Hildur Arnardottir (Center for Experimental Therapeutics and Reperfusion Injury, Brigham & Women’s Hospital) for the milk exudate data and analysis. This study was supported by National Institutes of Health grant P01GM095467.
Author information
Authors and Affiliations
Contributions
J.W.W., S.K.O., C.C.C., J.M.S., J.D. and N.C. designed, carried out and analyzed experiments as well as contributed to the manuscript and figure preparations; J.W.W. and N.A.P. synthesized synthetic RvD4; C.N.S. carried out overall experimental design and contributed to manuscript and figure preparations.
Ethics declarations
Competing interests
CNS is an inventor on patents [resolvins] assigned to BWH and licensed to Resolvyx Pharmaceuticals. CNS is a scientific founder of Resolvyx Pharmaceuticals and owns equity in the company. CNS’ interests were reviewed and are managed by the Brigham and Women’s Hospital and Partners HealthCare in accordance with their conflict of interest policies. NAP is an inventor on patents assigned to the University of Southern California licensed for clinical development and retains stock in Resolvyx Pharmaceuticals.
Rights and permissions
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
About this article
Cite this article
Winkler, J., Orr, S., Dalli, J. et al. Resolvin D4 stereoassignment and its novel actions in host protection and bacterial clearance. Sci Rep 6, 18972 (2016). https://doi.org/10.1038/srep18972
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/srep18972
This article is cited by
-
Role of specialized pro-resolving lipid mediators and their receptors in virus infection: a promising therapeutic strategy for SARS-CoV-2 cytokine storm
Archives of Pharmacal Research (2021)
-
Periodontitis: a Host-Mediated Disruption of Microbial Homeostasis
Current Oral Health Reports (2020)
-
Can Specialized Pro-resolving Mediators Deliver Benefit Originally Expected from Fish Oil?
Current Gastroenterology Reports (2018)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.