Potent Antibacterial Nanoparticles against Biofilm and Intracellular Bacteria

The chronic infections related to biofilm and intracellular bacteria are always hard to be cured because of their inherent resistance to both antimicrobial agents and host defenses. Herein we develop a facile approach to overcome the above conundrum through phosphatidylcholine-decorated Au nanoparticles loaded with gentamicin (GPA NPs). The nanoparticles were characterized by scanning electron microscopy (SEM), dynamic light scattering (DLS) and ultraviolet−visible (UV−vis) absorption spectra which demonstrated that GPA NPs with a diameter of approximately 180 nm were uniform. The loading manner and release behaviors were also investigated. The generated GPA NPs maintained their antibiotic activities against planktonic bacteria, but more effective to damage established biofilms and inhibited biofilm formation of pathogens including Gram-positive and Gram-negative bacteria. In addition, GPA NPs were observed to be nontoxic to RAW 264.7 cells and readily engulfed by the macrophages, which facilitated the killing of intracellular bacteria in infected macrophages. These results suggested GPA NPs might be a promising antibacterial agent for effective treatment of chronic infections due to microbial biofilm and intracellular bacteria.


Results and Discussion
Characterization of GPA NPs. The water solubility of the nanoparticles suggests that the charged polar head group of phosphatidylcholine (PC) is accessible on the outer surface of PA NPs 25 , which is beneficial for aminoglycoside binding. SEM images of the phosphatidylcholine-decorated Au nanoparticles alone (PA NPs) or loaded with gentamicin (GPA NPs) showed that there was no visible morphological difference (Fig. 1A,B). The average diameter of the GPA NPs was estimated to be ~180 nm by Image J software (Fig. 1C). An explicit increase of about 65 nm in the hydrodynamic size of the Au NPs was detected after PC coating via dynamic light scattering (DLS) (Fig. 1D), suggesting the formation of lipid bilayers. The binding of gentamicin seemed not to influence the size of nanoparticles further (Fig. 1D). The surface zeta potential changed from − 34.0 mV to − 24.7 mV (Fig. 1E), which confirmed the binding of positively charged gentamicin to the charged polar head group of phosphatidylcholine on PA NPs through electrostatic attraction. Both UV− visible spectra of PA NPs and GPA NPs revealed an identical absorbance peak at 530 nm, which suggested gentamicin load had no influence on the surface plasmon resonance of PA NPs (Fig. 1F). The binding amount of gentamicin on the GPA NPs was estimated to be ∼ 38 μ g/mg (gentamicin/Au).
The stability of PA NPs was examined by adding various amounts of gentamicin (stock solution 10 mg/mL). As shown in Figure S1A and S1B, addition of gentamicin to PA NPs did not lead to a distinguishable color change. In contrast, upon addition of gentamicin, the color of Au NPs initially changed from wine red, then to purple, and finally to violet blue (Figure S1C), coinciding with the red shift in UV-vis spectra( Figure S1D), which corresponded to a typical signature of a fast nanoparticles aggregation 26,27 . These results indicated that PC coating made Au NPs more stable to gentamicin. Again, these data suggested that for PA NPs, all Au NPs were coated with PC. Otherwise, gentamicin addition would lead to the red shift in UV-vis spectra ( Figure S1C and S1D).
The absorption of gentamicin on PA NPs reached the saturation point in 2 h at both acidic and neutral pH ( Fig. 2A). It appeared that the acidic condition facilitated the binding of the antibiotic. The increase of ionic strength led to a dramatic decrease in the loading of gentamicin on PA NPs (Fig. 2B). The release profile of gentamicin from GPA NPs indicated that GPA NPs were more stable at acidic pH (Fig. 2C) and lower ionic strength (Fig. 2D). The influence of pH and ionic strength implied that the binding of gentamicin to PA NPs was most probably under the control of electro-static interactions.
Antibiofilm activities of GPA NPs. According to the minimum inhibitory concentration (MIC) assay, GPA NPs were effective against planktonic bacteria as gentamicin did (Table S1). The binding affinity of GPA NPs toward planktonic bacteria was further examined. Figure S2 displayed the photographs obtained after vortex mixing the GPA NPs alone and GPA NPs with different bacteria. Precipitates were observed in all samples except GPA NPs alone, which indicated the binding between the GPA NPs and the bacteria existed. The antibiofilm efficacy of the particles was evaluated against both gram-positive and -negative microorganism. A standard crystal violet assay for biofilm biomass indicated that GPA NPs were more effective in eradication of preformed biofilm built by P. aeruginosa or S. aureus (Fig. 3), than gentamicin or PA NPs alone did. The similar findings were also observed in case of biofilm built by E. coli ( Figure S3A) and L. monocytogenes ( Figure S3B). Visualization of bacterial biofilms with scanning electron microscopy and fluorescence microscopy showed a wide spectrum of morphological differences in biofilm architectures ( Fig. 4 and S4). Notably, very few scattered cell aggregates were observed in the biofilms and there were less viable cells in the aggregates after 24 h exposure to the GPA NPs.
Besides, an equivalent amount of gentamicin (as in GPA NPs) was added to Au NPs, and no significant difference in the ability to disrupt biofilm was observed between gentamicin and gentamicin-carried gold nanoparticles (GA NPs) ( Figure S5), which is in consistent with the earlier findings indicating no enhancement of the bactericidal activity of similar gentamicin-Au nanoparticles 26 . Thus the antibiofilm activity of GPA NPs could unambiguously be attributed to the presence of phosphatidylcholine coated Au NPs loaded with gentamicin.
Inhibition of biofilm formation was also examined in case of planktonic P. aeruginosa (Fig. 5A) and S. aureus (Fig. 5B) exposed to reagents for 24 h at the beginning. Quantification of biofilm biomass indicated that GPA NPs were superior to inhibit the biofilm formation of both bacteria above. The similar findings were also observed in case of E. coli ( Figure S6A) and L. monocytogenes ( Figure S6B). Taken together, these results demonstrated that GPA NPs were more effective to inhibit biofilm formation and disrupt preformed biofilms, regardless of Gram-positive or Gram-negative organisms than gentamicin did.
The activities of GPA NPs against intracellular bacteria. Macrophages function at the front line of immune defences against incoming pathogens, and therefore, are a common target for those bacterial pathogens that benefit from avoiding an encounter with the immune system, as well as those that are aiming to secure systemic spread 6 . The capability of GPA NPs against intracellular bacteria in infected macrophages was evaluated.
Viability tests indicated that the toxicity of the GPA NPs towards macrophages was negligible ( Figure S7A). There were no obvious differences in cell morphologies between the blank and GPA NPs treatments ( Figure S7B). Macrophages had shown to engulf NPs 23,24 , we first examined whether the macrophages (RAW 264.7) could readily engulf GPA NPs. Fluorescence and brightfield imaging GPA NPs were extensively engulfed to the cytoplasm of the cells after 2 h incubation ( Fig. 6A-D). Quantification of GPA NPs via inductively coupled plasma mass spectrometry (ICP-MS) demonstrated that the cellular uptake of GPA NPs increased in a time-dependent manner (Fig. 6E).
Pathogens such as P. aeruginosa and L. monocytogenes, are known to survive inside macrophages 28,29 , which were used as the model system to explore the intracellular killing activity of GPA NPs. As shown in Fig. 7, the treatment of infected macrophages with GPA NPs resulted in a more dramatic decrease of live P. aeruginosa in those cells than gentamicin did, The similar observation was in case of macrophages infected with L. monocytogenes ( Figure S8).

Conclusion
In this study, a simple and facile method was developed to generate stable GPA NPs. These GPA NPs could effectively eradicate preformed biofilm and inhibit the biofilm formation, regardless of Gram-positive and Gram-negative pathogenic bacteria. In addition, GPA NPs had good biocompatibility and elicited a superior intracellular killing capability against multiple pathogenic bacteria in infected macrophages. The strategy may be useful to develop new therapeutics for treating chronic and stubborn infections related with biofilm and intracellular bacteria.

Materials. Gentamycin Sulfate was purchased from Solarbio (Beijing, China). Phosphatidylcholine and
Hydrogen tetrachloroaurate (III) were purchased from Aladdin (Shanghai, China). Citric acid monohydrate was purchased from Sinopharm Chemical Reagent Co., Ltd. All reagents were of analytical grade and used as received without further purifying.
Preparation of GPA NPs. Figure 8 showed the synthesis of phosphatidylcholine-decorated Au nanoparticles (PA NPs) 25 . Briefly, Phosphatidylcholine (0.065 g, 0.084 mmol) in CHCl 3 (10 ml) was added to a glass vial and the solvent was removed by rotary evaporation to provide a thin film 30 . Then the film was dried at room temperature for 12 h to ensure that all the CHCl 3 was removed. Ultra-pure H 2 O (10ml) was added to the vial, shaken and  10 kV. To obtain high resolution images from the SEM analysis, all samples were deposited on a silicon wafer and allowed to dry. The SEM images were processed using the Image J software, and the size histograms were constructed from an analysis of 1000 particles. The hydrodynamic size and surface zeta potential were measured by dynamic light scattering (DLS) measurements (Malvern Zetasizer NANO-ZS90). The UV-visible absorption spectra were recorded on Thermo Evolution 300 spectrophotometer in the range of 300-800 nm. The gentamicin content was determined by Sodium phosphotungstate precipitation method.

Gentamicin Load Capacity and Release Behaviors.
The load capacity, defined as the ratio of the amount of gentamicin binding on the nanoparticles to the initial amount of gentamicin introduced, was determined by mixing prepared PA NPs with 0.2 mg/mL gentamycin at different pH and the supernatant was obtained for measurement of free-form (no-load) gentamycin after centrifugation.
The kinetics of gentamicin release was studied from the prepared GPA NPs. In order to determine the effect of pH on the gentamicin release profiles of nanoparticles, 6 mM HEPES buffer (pH 7.4) and 6 mM Tris-HCl buffer (pH 4.5) were used respectively. The 1 mL fresh prepared nanoparticles solution (which was incubated with gentamicin for 3 h) was initially centrifuged at 14 000 rpm at 10 °C for 60 min and the precipitate was rinsed with UP water (1 mL). Then the GPA NPs were resuspended in 1 mL buffer at 20 °C in tube. One tube was taken at regular time intervals (1, 2, 3, 4, 6, and 7 days), centrifuged and the supernatant was obtained for gentamicin measurement.
Examination of Binding Affinity. GPA NPs (0.116 mg/mL, Au basis) were mixed and shaken (37 °C, 160 rpm) with bacterial samples prepared in TSB for 2 h. The samples were centrifuged at 3000 rpm for 10 min, and the binding affinity was examined by the naked eye.  Cytotoxicity Tests. The RAW 264.7 cell line was cultured in RPMI medium supplemented with 10% FBS, 100 μ g/mL penicillin and 100 μ g/mL streptomycin at 37 °C in a humidified 5% CO 2 -contaning balanced-air incubator.
Cell viability was estimated through MTT assay. The 200 μ L cells (~8000 cells) were incubated for 12 h in 96-well plates, then the medium was replaced with the medium containing different concentrations of GPA NP and incubated for another 6 h. Cells incubated in the medium containing no GPA NPs were used as a negative control (Blank). After treatment, the media containing sample was changed with fresh media and 10 μ L of MTT (5 mg/mL) was added and the incubation continued for 4 h. Medium was removed, and 100 μ L of DMSO was added to each well to dissolve the formazan. The absorbance was measured at 570 nm. Moreover, cells grown on glass coverslips in 24-well plate with the same treatment were fixed with 4% paraformaldehyde in PBS for 10 min. Images were obtained using a microscope (Olympus, Tokyo, Japan). Cellular uptake of GPA NPs. The qualitative localization of GPA NPs within cells was observed by microscopy, while the uptake of GPA NPs by cells was quantified by inductively coupled plasma mass spectrometry (ICP-MS). RAW264.7 macrophages were plated on coverslips 12 h at 37 °C. Then the medium was replaced by new medium containing GPA NPs (0.116 mg/mL) for 2 h. The medium was then removed, the coverslips were rinsed three times with PBS, fixed with 4% paraformaldehyde and stained with Hoechst. The coverslips were imaged by Olympus BX53 upright microscope with a 100 × (1.30) plan oil immersion objective lens.
For the ICP-MS measurements, 10 6 RAW264.7 cells were seeded in 6 well plates in 2 mL of complete culture medium. After 12 h, cells were incubated with 0.116 mg/mL of GPA NPs for 1, 2, 4, and 8 h. Then the medium was removed and cells were washed three times with PBS. Cells were trypsinized, counted and harvested. Cells suspension was centrifuged at 200 × g for 5 min, the supernatant was transferred to a new tube. Cell pellets were digested with Aqua Regia (400 μ L) and microwave (2 cycles at 950 W for 10 min). Plasma conditions and gold element identification were set as described 31 .
Activity of the GPA NPs toward Intracellular Bacteria. RAW 264.7 cells were plated overnight on 24-well plate. Cells were infected at an MOI = 10 for 1 h, washed thrice with PBS, and medium with 50 μ g/ml gentamicin was added for another 1 h. After washed thrice with PBS, cells were incubated in 1 mL fresh medium supplemented with GPA NPs (0.116 mg/mL), equivalent gentamicin (Gen) or PA NPs for 2 h. The medium was then removed and the remaining species were rinsed three times with PBS. Subsequently, the medium was replaced by new medium free penicillin-streptomycin and incubated at 37 °C for 12 h. The macrophage samples were diluted 10 4 (L. monocytogenes) or 10 5 (P. aeruginosa) times serially by PBS solution. The resultant solution (0.1 mL) was directly cultured on a Petri dish containing TSB agar at 37 °C for 16 h and the colony-forming units (CFU) were counted. Antibiofilm Activity. As described previously 32 , 100 μ L bacterial TSB solutions (~10 8 CFU) were seeded into 96-well polystyrene microtitre plates (Corning, NY, USA) at 37 °C for 24 h to allow biofilm formation. The non-adhered cells were removed with pipette and the plate was washed three times using 100 μ L 0.9% (w/v) NaCl.   Then existing biofilms were incubated at 37 °C in 90 μ L TSB supplemented with 10 μ L GPA NPs (0.116 mg/mL), equivalent Gen or PA NPs for 24 h. Each treatment included 6 parallel wells. Biofilms incubated with TSB only were used as blank. Biofilm mass was evaluated by Crystal violet staining assay. All experiments were performed 3-5 times. Error bars represent SD.
For biofilm inhibition assay, 100 μ L of bacteria in TSB (approximately 10 8 FU) were seeded into individual wells of microtiter plates in the presence of compounds for 24 h. Biofilm mass were evaluated as described above.
For fluorescence microscopy, S. aureus or P. aeruginosa (~10 8 CFU) was grown on glass coverslips at 37 °C for 24 h in 24-well plates supplemented with 1 mL of TSB to allow biofilm formation. The coverslips were washed to remove unattached cells and were treated with GPA NPs, equivalent Gentamicin or PA NPs for 24 h at 37 °C. Existing biofilms were treated and imaged as previous 32 . SEM was conducted as described previously 11 .
Statistical analysis. All graphical evaluations were made using GraphPad Prism 5.0 (GraphPad Software Inc., San Diego, CA). Analysis of variance (ANOVA) was used to evaluate significant differences.