Figure 6 : Germinal centre response after prime vaccination.

From: Enhancing immunogenicity and transmission-blocking activity of malaria vaccines by fusing Pfs25 to IMX313 multimerization technology

Figure 6

BALB/c mice were immunised i.m. either with 1X108 i.u. ChAd63 or 2.5 μg protein formulated in Alhydrogel for Pfs25 and Pfs25-IMX313. Nine days and 14 days post-vaccination the inguinal dLNs (A) and spleen (B) were harvested and surface stained as outlined in the methods section. The mean and individual % of GL7+CD95+ cells in the B220+ cell population are shown (gating strategy shown in Figure S3). On day 14 after protein-in-adjuvant immunisation, the inguinal dLNs were also sectioned and stained with fluorescence labelled anti-GL7 (green) and anti-B220 (red) antibodies. Representative sections from each group are shown (C). Mann-Whitney test was performed *p < 0.05, ***p < 0.001.