Figure 3 : Reactivity of vaccine-induced antibodies to native parasite antigen.

From: Enhancing immunogenicity and transmission-blocking activity of malaria vaccines by fusing Pfs25 to IMX313 multimerization technology

Figure 3

Ookinetes of P. berghei transgenic for Pfs25 (Pfs25DR3) were stained with pooled sera from mice that received ChAd63-MVA expressing Pfs25 or Pfs25-IMX313 (Fig. 2B), and mice that received 3 doses of 2.5 μg Pfs25 or Pfs25-IMX313 formulated in Alhydrogel (Fig. 2C). Anti-Pfs25 mAb 4B7 was included as the positive control and sera from mice which received ChAd63-MVA expressing GFP or three doses of OVA protein formulated in Alhydrogel were included as negative controls. Antibody binding was detected by Alexa Fluor® 488-conjugated goat anti-mouse IgG (green) (lane 1) and the DNA was stained with DAPI (blue) (lane 2). Merged view (lane 3) as well as bright field (lane 4) are also shown.