Figure 4 : Histological analysis of the re-epithelialization and granulation of wounds 3 and 7 days after treatment.

From: Potency of umbilical cord blood- and Wharton’s jelly-derived mesenchymal stem cells for scarless wound healing

Figure 4

(a) Representative images of wound tissues with HE staining 3 days after treatment. The original wound margins (indicated with arrows) and the re-epithelialization edges (arrowheads) were carefully identified under the microscope. The distances between two wound margins (B; wound lengths) and between two re-epithelialization edges (A) were measured. The images of whole wound tissues were taken under a low power lens (40×, scale bars 200 μm). The inset high-power (200×) images indicate the epithelial tongues (surrounded by the dotted white lines) of the wounds. (b) Quantitative data on the distances between the re-epithelialization edges 3 days after treatment. (c) The ratio of re-epithelialization was measured by: [the wound length (B) minus the distances between the re-epithelialization edges (A)]/B × 100. (d) Representative images of wound tissues with HE staining 7 days after treatment. The granulation area was surrounded by the dotted black lines (40×, scale bars: 200 μm). (e) The area of granulated tissue was measured by Adobe Photoshop CS6 Extended software.