Quantification of anthocyanins and flavonols and transcription levels of flavonoid biosynthetic genes in the leaves, stems and developing storage roots (S16) of 2-month-old wild-type and Lc transgenic sweet potato.
(A,B) Changes in the anthocyanin content (A) and flavonol (quercetin-3-O-hexose-hexoside and quercetin-3-O-glucoside) content. (B) WT, wild type; Lc1–3, independent Lc transgenic lines. (C) Transcription levels detected by qRT-PCR analysis. The sweet potato ACTIN gene was used as an internal control. PAL, phenylalanine ammonia lyase; CHI, chalcone isomerase; CHS, chalcone synthase; F3H, flavanone 3-hydroxylase; FLS, flavonol synthase; DFR, dihydroflavonol 4-reductase; ANS, anthocyanidin synthase; 3GT, UDP-glucose:flavonoid 3-O-glucosyltransferase. Error bars represent the SE of three replicates. Asterisks indicate a significant difference compared to the WT at *P < 0.05 or **P < 0.01 (t-test).