Figure 5 : LesA elicits HR-like symptoms in grapevine leaves.

From: The Type II Secreted Lipase/Esterase LesA is a Key Virulence Factor Required for Xylella fastidiosa Pathogenesis in Grapevines

Figure 5

Crude protein from LesA-expressing E. coli was isolated and syringe-infiltrated in grapevine leaves of greenhouse-grown plants. (A) HR-like symptoms appeared 24 h after infiltration. (B) No reaction was observed after infiltration of crude protein from E. coli expressing the empty vector (pJexpress 401). (C) Reduced HR-like symptoms caused by S200A-LesA, in which the residue Ser-200 from Xff LesA catalytic triad was replaced by alanine. Wild-type LesA caused the same necrosis shown in A. Pictures of leaves were taken by Hossein Gouran.