Figure 3 | Scientific Reports

Figure 3

From: Characterization of Social Behaviors in caspase-3 deficient mice

Figure 3

Casp3−/− mice show sociability and preference for social novelty in three-chamber tests.

(A,C,E,G) Sociability test. Graphs show (A) total distance traveled (p < 0.0001 for genotype, p = 0.5799 for sex, p = 0.4020 for genotype × sex, by two-way ANOVA); (C) time spent in each side chamber containing novel mouse or empty wire cup (novel object), or the center chamber (p < 0.0001 for chamber, p = 0.4877 for genotype, p = 0.0298 for sex, p = 0.3169 for chamber × genotype × sex, by three-way ANOVA); (E) preference for novel mouse (p = 0.0431 for genotype, p = 0.0007 for sex, p = 0.1196 for genotype × sex, by two-way ANOVA), calculated as [(time spent exploring novel mouse)/(total time spent exploring novel mouse and novel object)] × 100%; ((G), left) time spent exploring novel mouse (p = 0.9450 for genotype, p = 0.0006 for sex, p = 0.9992 for genotype × sex, by two-way ANOVA); ((G), right) time spent exploring novel object (p = 0.0190 for genotype, p = 0.0088 for sex, p = 0.0237 for genotype × sex, by two-way ANOVA). (B,D,F) Social novelty test. Graphs show (B) total distance traveled (p < 0.001 for genotype, p = 0.9251 for sex, p = 0.7376 for genotype × sex, by two-way ANOVA); (D) time spent in each side chamber containing novel mouse 1 (now familiar), or novel mouse 2, or the center chamber (p < 0.0001 for chamber, p = 0.2572 for genotype, p = 0.0554 for sex, p = 0.0230 for chamber × genotype × sex, by three-way ANOVA); (F) preference for novel mouse 2 (p = 0.9113 for genotype, p = 0.8266 for sex, p = 0.0477 for genotype × sex, by two-way ANOVA); calculated as [(time spent exploring novel mouse 2)/(total time spent exploring novel mouse 1 and novel mouse 2)] × 100%. Graphs show mean ± SEM for 20 WT males, 20 Casp3−/− males, 20 WT females and 19 Casp3−/− females. **p < 0.01, ***p < 0.001, ****p < 0.0001, for genotype effect within each sex for (A,B,EG), by Bonferroni post-hoc tests following two-way ANOVA with the factors of genotype and sex.; for chamber effect between novel mouse vs. novel object in (C) and for novel mouse 1 vs. novel mouse 2 in (D), by Tukey post-hoc test following three-way ANOVA with the factors of genotype, sex and chamber side (novel mouse 1 side vs. the opposite side).

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