Table 2: BDNF ELISA kits performance.

From: A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays

Number of plates tested: 2Aviscera-Bioscience (n = 38)Biosensis (n = 39)Millipore ChemiKineTM(n = 39)Millipore Milliplex® (n = 38)Promega Emax® (n = 40)R&D System Quantikine® (n = 40)
Range ng/ml: min-max14.1–32.413.6–41.414.5–58.58.7–32.412.1–27.515.4–46.2
Median ng/ml (25%–75%)20.7 (18.5–24.4)25.4 (21.5–32.0)23.0 (18.6–25.9)18.2 (14.2–22.2)19.0 (16.9–21.7)25.5 (21.0–30.5)
Declared intra-assay CV4–6%2–6%4%< 10%2–9%4–6%
Tested intra-assay CV2%–3%1%–1%2%–3%13%–14%3%–7%5%–6%
Declared inter-assay CV8–10%5–8%9%<10%<9%8–11%
Tested inter-assay CV12%5%13%18%16%20%
Tested processing time7 hours4 hours22 hours20 hours24 hours5 hours
  1. Comparison between declared performances and actual tested performances of the specified BDNF ELISA kits. Median and range values, intra- and inter-assay coefficients of variation (CV) were assessed by measuring BDNF serum level from healthy volunteers (n = 38–40). All the kits, except Milliplex®, which is based on multiplex technology (Luminex®/xMAP®), are based on classical sandwich ELISA.