Figure 5 : Co-detection of viruses with specific oligofluorophores.

From: A novel multiplex isothermal amplification method for rapid detection and identification of viruses

Figure 5

Two fluorogenic oligonucleotides (6-FAM/BHQ1-HIVLRp and 6-FAM/BHQ1-HCV-LRp) were simultaneous applied in multiplex reaction containing all the primers in Table 1 for detection of only hepatitis C virus (HCV) and human Immunodeficiency virus (HIV) nucleic acid extracts. Fluorospectrophotometric results demonstrate brighter fluorescence and higher RFUs in the HCV samples (lanes 5 and 6) and the HIV (lanes 9 and 10) as compared with the no-template controls (NTCs) in lanes 1 and 2, hepatitis B virus (HBV) in lanes 3 and 4, and hepatitis E virus (HEV) in lanes 7 and 8; UV = ultraviolet naked-eye visualization.