Representative space-resolved Raman spectra of a dividing HCT116 cell.
Raman spectra (A) (red line) and (B) (blue line) were measured, respectively, at two locations (A) and (B) indicated in the optical image of the cell; location (A) is closer to the cleavage furrow, whereas (B) is around the central part of a daughter cell (i.e., the nucleus). The 1900–2600 cm−1 region has been omitted because no Raman band is observed there. Both spectra were recorded with a 60-s exposure time and 3-mW laser power. Scale bar, 10 μm.